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2020 Fiscal Year Final Research Report

Are the immotile nodal cilia in the mouse embryo mechanosensors?: experimental tests using micromanipulation and calcium imaging

Research Project

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Project/Area Number 19K23761
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0702:Biology at cellular to organismal levels, and related fields
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

KATOH TAKANOBU A.  国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 (80844935)

Project Period (FY) 2019-08-30 – 2021-03-31
Keywords左右体軸形成 / ノード不動繊毛 / 光ピンセット / メカノセンサー / カルシウムイメージング
Outline of Final Research Achievements

The "difference between the left and right sides of the body", such as the heart being on the left side in mammals, is determined in the early stages of development. From previous studies, it has been found that the signal from the organelle "nodal immotile cilia", which exists in the "node" that appears transiently in the early embryo, is the first step to determine the left-right axis. However, it was not clear whether the nodal immotile cilia were mechanosensors that sensed "physical stimuli" or chemosensors that sensed "chemical stimuli." In this study, we applied the technologies of the optical tweezers and the calcium imaging to nodal-immotile cilia, and obtained results that strongly suggest nodal-immotile cilia is mechanosensors.

Free Research Field

生物物理学

Academic Significance and Societal Importance of the Research Achievements

これまで、ノード不動繊毛において変形による化学状態変化を明確に示した研究は報告されていない。本研究による成果は、十年来にわたる “ノード不動繊毛はメカノセンサーであるか否か” という論争に決着をつける重要な報告である。特に、ノード不動繊毛の異常は繊毛病と総称される病態の原因となっており、本研究で得られた知見は医学的・社会的に非常に意義があるものである。さらに、本研究で開発した顕微鏡技術はノード不動繊毛だけにとどまらず様々なサンプルに適用可能である。

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Published: 2022-01-27  

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