2020 Fiscal Year Final Research Report
Mechanisms underlying the giant bouton development of corticothalamic projections
| Project/Area Number |
19K23786
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0704:Neuroscience, brain sciences, and related fields
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
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| Project Period (FY) |
2019-08-30 – 2021-03-31
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| Keywords | 大脳皮質 / 視床 / 発生 |
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| Outline of Final Research Achievements |
Cerebral cortical layer 5 provides major outputs to subcortical areas. In this study, I sought factors that regulate the formation of giant boutons formed by layer 5 corticothalamic projections. RNA was purified from thalamus at different postnatal days, and transcriptome analysis was performed using RNA-sequencing. This identified genes that are up- or down-regulated during the second and third postnatal weeks. I also set the experimental conditions for in utero electroporation of plasmids into thalamic nuclei to perform functional analysis of candidate genes.
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| Free Research Field |
神経発生学
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| Academic Significance and Societal Importance of the Research Achievements |
大脳皮質5層からの入力を受ける視床領域は高次視床核と呼ばれ、感覚情報の統合を仲介する重要な働きをすることが示唆されてきた。しかし、その神経回路の形成過程については不明な点が多い。本研究で得られた成果は、今後、生後期の大脳皮質の活動に依存的した皮質下領域のシナプス成熟の機構を解明する上で重要である。類似のシナプス終末の構造は海馬にもあり、記憶の形成に重要である。本研究で得られた視床のシナプス終末形成制御の知見を活かすことにより、海馬の記憶形成の回路基盤についても理解が深まることが期待される。
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