Role of TL1A-DR3 in intestinal epithelial cells

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K23857
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0803:Pathology, infection/immunology, and related fields
• Research Institution: Akita University
• Principal Investigator: Shimodaira Yosuke 秋田大学, 医学系研究科, 助教 (20777982)
• Project Period (FY): 2019-08-30 – 2021-03-31
• Keywords: TL1A / 腸管上皮細胞

Outline of Final Research Achievements

The expression of DR3 in Caco2 was confirmed by realtime PCR, and the expression of DR3 was knocked down using siRNA. Single Caco2 cell in culture media was proliferated more with TL1A. MTT assay also confirmed proliferation under TL1A ltreatment, though LDH assay did not confirm an increase in cell death under TL1A treatment. Furthermore, gene expression alteration with TL1A was analyzed by RNA sequence, and alteration in expression of mitochondrial creatine kinase-related genes and peroxisome formation-related genes were confirmed.

Free Research Field

消化器内科学

Academic Significance and Societal Importance of the Research Achievements

TL1A-DR3サイトカイン-受容体シグナルは様々な炎症性疾患に関与していることが知られている。しかし、作用する細胞やまわりの環境によりその機能が異なる可能性があり、今回の研究では新たに腸管上皮細胞における役割が明らかとなった。今回明らかになった結果に基づきさらに研究展開が行われることで、腸炎のメカニズムが明らかとなり、治療応用などがなされることが期待される。