2019 Fiscal Year Research-status Report
Generation of universal donor cell souse for adoptive T cell therapy using iPSC technology
| Project/Area Number |
19K23863
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| Research Institution | Kyoto University |
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Principal Investigator |
王 博 京都大学, iPS細胞研究所, 研究員 (80842765)
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| Project Period (FY) |
2019-08-30 – 2021-03-31
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| Keywords | iPSCs / Activated iPS-T cells / PVR |
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| Outline of Annual Research Achievements |
Last year, I perfomed the screening of NK cell activating ligands expression level in iPSC derived T cells. By comparing the expression level with resting iPS-T cells, I found a candidate ligand, PVR were unregulated when iPS-T cells were activated. In fact, upon activation, all generated iPS-T cells iPSCs showed much more antigenicity to NK cells.So I desinged the PVR Knock out gRNA and generated PVR KO iPSCs. Then I checked that this PVR KO iPSCs could comparable differentiated into T cells with their parental iPSCs.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
Based on NK cell activation knowledge, we checked this on iPS-T cell situation and found the candidate factor.
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| Strategy for Future Research Activity |
Next step, I will check wether the PVR KO would affect the function of iPSCs derived T cells in vitro and in vivo first. Then investigate wether PVR KO iPS-T cells decrease the NK cell activation comparing the RVR express iPS-T cells. The fate of PVR KO iPS-T cells also will be planed to check in a human PBMCs transplated MHC dKO immunodeficiency mice model.
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| Causes of Carryover |
次年度使用額が生じた理由は、既述の通り研究の前の予定より少し遅れている部分があり、予備実験の検討など予測より時間と消耗少なかったためです。本年度は昨年度に実施できなかった研究内容も併せて実施する。そこで翌年度分として請求した助成金と合わせて使用する計画である。
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Research Products
(3 results)
