2020 Fiscal Year Annual Research Report
Generation of universal donor cell souse for adoptive T cell therapy using iPSC technology
| Project/Area Number |
19K23863
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| Research Institution | Kyoto University |
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Principal Investigator |
王 博 京都大学, iPS細胞研究所, 研究員 (80842765)
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| Project Period (FY) |
2019-08-30 – 2021-03-31
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| Keywords | immune rejection / iPSC-derived T cells / gene editing |
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| Outline of Annual Research Achievements |
We generated hypoimmunogenic cancer-antigen-specific T cells derived from induced pluripotent stem cells (iPSCs) lacking β2-microglobulin, the class-II major histocompatibility complex (MHC) transactivator and the natural killer (NK) cell-ligand poliovirus receptor CD155, and expressing single-chain MHC class-I antigen E. In mouse models of CD20-expressing B-Lymphoblastoid cells, differentiated T cells expressing a CD20 chimeric antigen receptor largely escaped recognition by NKG2A+ and DNAM-1+ NK cells and by CD8 and CD4 T cells in the allogeneic recipients while maintaining antitumour potency.
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[Journal Article] Generation of hypoimmunogenic T cells from genetically engineered allogeneic human induced pluripotent stem cells2021
Author(s)
Bo Wang, Shoichi Iriguchi, Masazumi Waseda, Norihiro Ueda, Tatsuki Ueda, Huaigeng Xu, Atsutaka Minagawa, Akihiro Ishikawa, Hisashi Yano, Tomoko Ishi, Ryoji Ito, Motohito Goto, Riichi Takahashi, Yasushi Uemura, Akitsu Hotta and Shin Kaneko
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Journal Title
Nature Biomedical Engineering
Volume: in press
Pages: in press
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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