Analysis of the role of UBD-dependent intracellular protein regulation in the pathogenesis of inflammatory bowel disease.

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K23990
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0903:Organ-based internal medicine and related fields
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Kawamoto Ami 東京医科歯科大学, 医学部附属病院, 特任助教 (70849124)
• Project Period (FY): 2019-08-30 – 2021-03-31
• Keywords: 炎症性腸疾患 / ユビキチン

Outline of Final Research Achievements

Our present study regarding the newly identified intracellular proteolysis system, UBD-dependent UBL-PSM system, identified 62 candidate target proteins by iTRAQ analysis. Also, in addition to p62, two additional candidate target proteins of the UBL-PSM system were revealed by immunoprecipitation analysis. These findings suggest that UBD, a protein induced under an inflammatory environment in human intestinal epithelial cells, may exhibit its function through multiple target proteins.

Free Research Field

消化器内科学

Academic Significance and Societal Importance of the Research Achievements

今回の結果より、ヒト腸上皮細胞に於いて炎症環境で発現誘導される分子UBDは、複数の標的分子を有する可能性が示された。同標的分子群の蛋白分解制御を介し腸上皮機能を調節するという新たな炎症性腸疾患の病態の可能性も提示した。標的分子のさらなる探索等を通じ、炎症性腸疾患における「UBD依存的UBL-PSM系」の理解が一層進展し、新規治療法の開発につながることが期待できる。