Investigation of novel treatment and cause of diseases in the field of pediatric surgery by deep proteome analysis of stools

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K24007
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0905:Surgery of the organs maintaining homeostasis and related fields
• Research Institution: The University of Tokyo
• Principal Investigator: Watanabe Eiichiro 東京大学, 医学部附属病院, 助教 (40623327)
• Project Period (FY): 2019-08-30 – 2021-03-31
• Keywords: 便 / プロテオーム / 腸内細菌 / 胆道閉鎖症 / 腸瘻 / 短腸症候群 / 胎便 / リンパ管腫

Outline of Final Research Achievements

We established deep proteome analysis of stools and apply it to infants with cholestasis, including both BA and non-BA cohorts. Our new method of deep proteome analysis by data-independent acquisition mass spectrometry (DIA-MS) can detect over 2,000 host-derived proteins in stools and provides a method for discovering new BA biomarkers. Further large-scale studies are needed to validate our results regarding the varying levels of host-derived stool proteins in BA as potential specific and reliable biomarkers for diagnosing this disease. Moreover, deep proteome analysis of stools has great potential to elucidate the pathophysiology of BA and other pediatric diseases, especially in the field of pediatric gastroenterology.

Free Research Field

便、小児外科

Academic Significance and Societal Importance of the Research Achievements

本研究により、BAの患児の便中には、特徴的なタンパク・プロファイルが存在することがわかった。今後もBA症例の蓄積と解析が重要と考えるが、これらを詳細に検討することが、BAの早期診断バイオマーカーの発見につながる可能性があると考える。また、かずさDAN研究所と共同して開発したDIAベースの便プロテオーム解析は、小児外科領域だけではなく、あらゆる消化器系疾患の新規バイオマーカー創出、更には新規治療法の開発や病因解明に大きな役割を果たすことが期待される。