2020 Fiscal Year Final Research Report
deltaNp63 is upregulated during salivary gland regeneration following duct ligation and irradiation in mice
Project/Area Number |
19K24070
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Multi-year Fund |
Review Section |
0907:Oral science and related fields
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Research Institution | Osaka University |
Principal Investigator |
Ikai Kazuki 大阪大学, 歯学研究科, 特任研究員 (00849432)
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Project Period (FY) |
2019-08-30 – 2021-03-31
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Keywords | 唾液腺 / 再生医療 / p63 / 導管結紮 / 放射線障害 |
Outline of Final Research Achievements |
The transcription factor p63, a component of the p53 family, has important functions in development, homeostasis, and regeneration of epithelial tissues. However, the role of p63 in the regeneration of exocrine glands, including the salivary glands (SGs), has not been fully investigated. We investigated p63 expression in SG regeneration induced by duct ligation and irradiation. The expression of deltaNp63, a p63 isoform, increased and was colocalized with keratin 5 positive cells were myoepithelial cells. Furthermore, deltaNp63 expression was regulated by FGF7 stimulation via p38 MAPK phosphorylation and affected SG morphogenesis. These results suggest that deltaNp63 is essential for SG regeneration and may be a new target for regenerative treatment.
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Free Research Field |
口腔科学
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Academic Significance and Societal Importance of the Research Achievements |
高齢者は様々な原因から口腔機能が低下するが、特に頻度の高い原因が口腔乾燥である。放射線照射やシェーグレン症候群により唾液腺が損傷を受けると、唾液の分泌量は減少しQOLが著しく低下する。腺機能を回復させる根本的な治療法はなく、唾液腺の再生医療が期待されている状況である。 本研究により、唾液腺の再生過程ではΔNp63とKeratin5両陽性の筋上皮細胞が重要な役割を果たしていること、ΔNp63の発現はFGF7刺激により誘導できることが明らかとなった。ΔNp63は唾液腺再生における有力な機能分子であり、再生治療の新しい標的となる可能性があると考えられる。
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