Elucidation of the anti-inflammatory effect of polyphosphoric acid for the establishment of treatment for periodontal disease

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K24079
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0907:Oral science and related fields
• Research Institution: Showa University
• Principal Investigator: Hasegawa Mikako (寺島実華子) 昭和大学, 歯学部, 助教 (00849408)
• Project Period (FY): 2019-08-30 – 2022-03-31
• Keywords: ポリリン酸 / LPS / 炎症性サイトカイン / 抗炎症作用 / 歯周炎

Outline of Final Research Achievements

We investigated the effect of polyP150 on a mouse model of cecal ligation and puncture (CLP) peritonitis that accurately reflects clinical sepsis, and showed that treatment with polyP150 significantly improved survival in a mouse model of CLP peritonitis. In addition, polyP150 suppresses CLP-mediated increase in pulmonary vascular permeability, and pretreatment of polyP150 in HMEC-1 cells, which are human vascular endothelial cells, is a tumor necrosis factor-α-induced monocyte THP-1 cell. It showed inhibition of adhesion and cell-cell adhesion molecule 1 / CD54 gene expression. These results suggest that polyP150 improves fatal sepsis by inhibiting the expression of cell adhesion molecules and the accumulation of leukocytes in the vascular endothelium, thereby suppressing the increase in vascular permeability.

Free Research Field

歯科保存学

Academic Significance and Societal Importance of the Research Achievements

リポ多糖(LPS)は、敗血症ショックなどの病態や歯周炎の発症に関与しており、医科領域と歯科領域に共通の重要な標的である。 本研究の結果は、polyP150が細胞接着分子の発現と血管内皮における白血球の蓄積を阻害し、それによって血管透過性の増加を抑制することにより、致命的な敗血症を改善することを示唆している。