2020 Fiscal Year Final Research Report
The function of BMP3b on bone metastasis focusing MRONJ
| Project/Area Number |
19K24126
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0907:Oral science and related fields
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| Research Institution | Kyushu Dental College (2020)
Saga University (2019) |
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Principal Investigator |
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| Project Period (FY) |
2019-08-30 – 2021-03-31
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| Keywords | 乳がん / 顎骨壊死 / MRONJ / BMP3b / GDF10 |
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| Outline of Final Research Achievements |
In this study, we used MCF-7, human breast cancer cells, and 4T1, murine breast cancer cells. The treatment of recombinant human (rh) BMP-3b decreased the proliferation capacity assessed by CCK-8 assay in these cells and also decreased the migration capacity of cells. The treatment of rhBMP-3b increased the protein levels of E-cadherin, the maker of epithelial lineage cells while rhBMP-3b decreased the Vimentin, the maker of mesenchymal lineage cells. Moreover, rhBMP-3b suppressed the phosphorylation of Smad3 induced by the treatment of TGF-β, suggesting that BMP-3b regulates proliferation and migration of breast cancer cells though the suppression of epithelial-mesenchymal transition induced by TGF-β signaling.
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| Free Research Field |
口腔外科学
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| Academic Significance and Societal Importance of the Research Achievements |
これまで乳がん細胞とBMP3bの関連に着目した研究はほとんど皆無であったことから，本研究の学術的独自性は高い．また本研究を発展させることで，乳がんの骨転移メカニズムの一端が明らかにできれば，新たな乳がん骨転移制御法の確立に貢献できる可能性がある．さらに，将来的にビスホスホネートなどのように骨代謝回転を低下させることなく骨転移をコントロールできるようになれば，MRONJの発症を減らすことが可能となり，現在歯科界で問題となっているMRONJの解決に大きく貢献できる．
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