2020 Fiscal Year Annual Research Report
The in vitro/in vivo study of CCN2 on dentin regeneration
| Project/Area Number |
19K24127
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
邱 友靖 北海道医療大学, 歯学部, 研究員 (90845421)
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| Project Period (FY) |
2019-08-30 – 2021-03-31
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| Keywords | dentin regeneration / dental materials / bioactive materials |
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| Outline of Annual Research Achievements |
Last year, I investigated the in vitro/in vivo effect of novel bioactive materials on rat odontoblast-like MDPC-23 cells, human dental pulp stem cells (hDPSCs), and wistar rats as planned.
Adhesive system has been one of the most important success elements for endodontic treatment. The investigation of a novel adhesive monomer CMET (a calcium salt of 4-methacryloxyethyl trimellitic acid) on MDPC-23 cells has revealed that CMET stimulated MDPC-23 cells proliferation, differentiation and mineralization. The findings suggested that CMET can promote intracellular Ca2+ homeostasis and provide a signal for activation of downstream events that promote odontoblast differentiation and matrix mineralization. The results have been published on on International Endodontic Journal (Int Endod J. 2020 Oct; 53(10):1413-1429).
The investigation on hDPSCs has showed that CMET has low cytotoxicity and exhibits high activity on stimulation of odontogenic differentiation and matrix mineralization. The in vivo study on wistar rats showed that direct capping with CMET can effectively promote dentin bridge formation.
The results of the effect of CMET on dentin regeneration is under submission to SCI journal and patent application.
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