2020 Fiscal Year Final Research Report
Elucidation of the effect of enhancing antitumor activity of CD8 + T cells by modifying cholesterol metabolism
| Project/Area Number |
19K24148
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0907:Oral science and related fields
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| Research Institution | Hiroshima University |
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Principal Investigator |
Mishima Takefumi 広島大学, 医系科学研究科(歯), 専門研究員 (70848473)
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| Project Period (FY) |
2019-08-30 – 2021-03-31
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| Keywords | 免疫治療 / LAK細胞 / ステロール代謝 / 免疫チェックポイント分子 / PD-1 / Zoledronic Acid / 顎骨壊死 |
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| Outline of Final Research Achievements |
The cytotoxic activity of LAK cells induced by adding various cholesterol synthesis inhibitors using a serum-free culture system was evaluated by a radioactive chromium release test, and PD-1 gene expression of each LAK cell was evaluated by quantitative PCR.
It was suggested that the amount of cholesterol in LAK cells is involved in the induction of cytotoxic activity of LAK cells, and that cholesterol metabolism is involved in the mechanism of PD-1 molecule expression.
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| Free Research Field |
外科系歯学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により，高い細胞障害活性を有する LAK 細胞を誘導するためには，LAK 細胞のコレ
ステロール量が重要であることが示唆された。さらに, がん免疫療法において PD-1 などの免疫疲弊分子の発現には免疫細胞の細胞膜コレステロールが重要な役割を担っていることが考えられ，今後，免疫細胞のコレステロール代謝制御による免疫療法の有用性が示唆された
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