2020 Fiscal Year Final Research Report
Approach for autologous tooth transplantation using organoid of periodontal tissue
Project/Area Number |
19K24158
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Multi-year Fund |
Review Section |
0907:Oral science and related fields
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Research Institution | Fukuoka Dental College |
Principal Investigator |
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Project Period (FY) |
2019-08-30 – 2021-03-31
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Keywords | 自家歯牙移植 / 歯周組織オルガノイド / スフェロイド / 細胞シート / コラーゲン培養 / PAI-1 / Cementum protein 1 / ヒト歯根膜幹細胞 |
Outline of Final Research Achievements |
To prepare the periodontal organoids that evoke an harmonious complex of regenerative periodontal tissue after autologous tooth transplantation, three-dimensional 83D) culture system have been applied in regenerative medicine strategies because they provide a convenient in vitro model for cell-cell and cell-matrix interaction. This study examined where the cementogenic differentiation of human periodontal ligament stem cells (HPLSCs) can be accelerated using 3D culture systems. In 2D culture system, HPLSCs treated with plasminogen activator inhibitor-1 (PAI-1) showed increased expression of cementum protein 1 (CEMP1) and cementum attachment protein (CAP), indicating that PAI-1 could induce cementogenesis in HPLSCs. In contrast, cementogenesis were enhanced in HPLSC spheroid embedded in collagen-gel treated with PAI-1. 3D culture of HPLSC, using spheroids, are more susceptible for PAI-1-induced comentognesis.
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Free Research Field |
歯科矯正学
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Academic Significance and Societal Importance of the Research Achievements |
予後の良い自家歯牙移植法には、歯周組織を構成する細胞群をin vitroにおいて組織化して移植と併用することが重要であると考えた。そのためには、コラーゲン培養法を組み合わせた3次元培養法により歯周組織オルガノイドの形成が必要である。このオルガノイドを併用した移植により、歯根膜を介した歯槽骨と移植歯セメント質からなる調和の取れた歯周組織複合体の再現され、確実な生着による機能回復が期待される。また、矯正歯科治療における便宜抜去された歯牙の欠損部への再利用も一般的な治療方法となる可能性がある。
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