2022 Fiscal Year Final Research Report
Study on mechanism of endothelial mechanotransduction by using micro-mechanical manipulation technique
| Project/Area Number |
19KK0276
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| Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 90:Biomedical engineering and related fields
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
東藤 正浩 北海道大学, 工学研究院, 教授 (10314402)
山田 悟史 北海道大学, 工学研究院, 助教 (90730169)
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| Project Period (FY) |
2019-10-07 – 2023-03-31
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| Keywords | 内皮細胞 / メカノトランスダクション / 一次繊毛 / 磁気ナノビーズ / 細胞間力 |
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| Outline of Final Research Achievements |
Cells may change their morphology and function in response to surrounding mechanical environment. Recently, it has been pointed out that primary cilia protruding from the surface of cells contribute to the sensing mechanism of mechanical stimuli, however, the detail of the mechanosensing mechanism remains unclear. The objective of this project was to estimate the mechanotransduction pathway of endothelial cells in response to flow stimuli using magnetic nanobead technology. Cellular responses to an externally applied magnetic field after attaching magnetic nanobeads to the cell surface was measured. It was found that the cells elongated in the direction of the magnetic field. A microfluidics system was also constructed to efficiently generate magnetic nanobead-introduced artificial cells. Furthermore, a measurement of mechanical properties of primary cilia was newly designed and developed.
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| Free Research Field |
バイオメカニクス
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| Academic Significance and Societal Importance of the Research Achievements |
本研究から得られる知見は内皮細胞の流れに対する力学応答の理解を一層深めるものであり、それはすなわち動脈硬化症など内皮細胞が関連した血管疾患の発生・発達の機序の解明そして診断技術の開発に貢献することが期待される。また、本申請内容は細胞の力学応答研究において新たな実験技術となり得る研究手法を提案・開発するため、細胞バイオメカニクスの新しい基盤技術として当該分野の発展に大きく資することができると考えられる。将来的には引き続き共同研究体制を維持しながらより臨床に役立つ知見を提供すべく、細胞の力学刺激に対する応答能を亢進させる薬剤や生化学的因子を用いた研究に進めていきたいと考えている。
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