2023 Fiscal Year Final Research Report
Axon initial segment plasticity in the jumping escape giant fiber neuron
| Project/Area Number |
19KK0383
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| Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research (A))
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| Allocation Type | Multi-year Fund |
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| Research Field |
Basic / Social brain science
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| Research Institution | Akita University (2023)
Tohoku University (2019-2022) |
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Principal Investigator |
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| Project Period (FY) |
2020 – 2023
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| Keywords | 軸索起始部 / ドーパミン受容体 |
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| Outline of Final Research Achievements |
In the research project, I examined the axon initial segment (AIS) and its role in neural output regulation and neural plasticity. One specific area of focus was the DopEcR, a dopamine receptor located in the AIS. Through molecular localization in a multinuclear neuroblast culture system, we found that DopEcR and NaV are co-localized in the AIS. Furthermore, experiments measuring the DLM flight muscle, which is part of the giant escape system, demonstrated the involvement of DopEcR, cAMP, and TRPV in promoting stimulus habituation. This suggests that DopEcR has an inhibitory effect on action potential generation through specific ion channels in the AIS. These findings provide valuable insights into the molecular basis of AIS plasticity and its potential implications for neurological and psychiatric disorders.
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| Free Research Field |
神経科学
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| Academic Significance and Societal Importance of the Research Achievements |
中枢神経系の馴化における個別のチャネル遺伝子の機能理解に繋がり、AISの制御メカニズムを含む神経可塑性の基礎的メカニズムの理解に貢献する。
ドーパミン受容体のアゴニスト、アンタゴニストは、さまざまな精神疾患の処方薬として多く用いられているが、副作用も多い。より安全かつ効果の高い新薬の開発のためにも、ドーパミン受容体の「細胞内効果」の十分な理解が必要である。本課題では、DopEcRの標的チャネルからその細胞内効果を分子レベルで特定し、これに貢献することを目指す。同方法論は、さまざまな分子機能の解析にも適用でき、異なる受容体を介したドーパミン作用の機能解析も加速できるものと期待している。
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