ペプチドニューロンによる神経回路機能修飾の分子・細胞メカニズム

2008 Fiscal Year Annual Research Report

• Project/Area Number: 20021012
• Research Institution: The University of Tokyo
• Principal Investigator: 岡 良隆 The University of Tokyo, 大学院・理学系研究科, 教授 (70143360)
• Keywords: 神経科学 / 生理学 / 脳・神経 / 動物 / 生理活性 / 神経修飾 / ペプチド / GnRH

Research Abstract

我々は1)新規ペプチド・キスペプチンによるGnRHニューロン制御が神経修飾系も含めたすべてのGnRH神経系に及び、生殖と性行動の調和のとれた制御をする、2)GnRHとキスペプチンというペプチド神経系が脊椎動物を通じて生殖以外にも重要な神経修飾系として働いている、という作業仮説を提唱している。これを実証するために、各種トランスジェニックメダカを作成してダブルパッチクランプなどの電気生理学的実験やニューロン特異的破壊のできる実験系などの最先端技術を確立し、これらのペプチド神経系による神経回路修飾の機構を分子から行動のレベルまで明らかにするための実験を計画した。本年度は次のような研究実績を上げた。1)キスペプチンニューロンがGFPを発現するトランスジェニックメダカ作成を完成間近まで近づけ、平成21年度にそれらを用いた形態学・生理学実験により、既に確立しているGnRHトランスジェニックメダカと合わせて、両ペプチド神経系が形成する神経回路の解析を可能にした。2)GnRHニューロンGFPトランスジェニックメダカを用いた3種のGnRHニューロンすべての電気生理学・形態学的特徴の解析に成功した。3)体の透明性の高いメダカの利点を最大限に活かし、強い緑色光の照射による励起で活性酸素を発生してその細胞のみに特異的に細胞死を起こさせるKillerRedタンパク質をGnRHニューロン・キスペプチンニューロンに遺伝子導入したトランスジェニックメダカの作成も完成間近まで近づけ、平成21年度に、それぞれのペプチドニューロンを時期・細胞特異的に破壊してその機能を解析するための実験系を準備した。4)GnRHペプチドによる神経回路修飾の実験モデル系として、キンギョ嗅球・嗅上皮のin vitro標本を開発した。

Research Products

• [Journal Article] Three types of gonadotrophin-releasing hormone neurones and steroid-sensitive sexually dimorphic kisspeptin neurones in teleosts (2009)
  • Author(s): Oka, Y.
  • Journal Title: Journal of Neuroendocrinology 21 Pages: 334-338
  • Peer Reviewed
• [Journal Article] Primary culture of the isolated terminal nerve-gonadotrophin releasing hormone(GnRH)neurones derived from adult teleost(dwarf gourami, Colisa Jolla)brain forthe study of peptide release mechanisms (2009)
  • Author(s): Abe, H., and Oka, Y.
  • Journal Title: Journal of Neuroendocrinology 21 Pages: 489-505
  • Peer Reviewed
• [Journal Article] Possible role of oestrogen in pubertal increase of Kiss1/Kisspeptin expression in discrete hypothalamic areas of femalerats (2009)
  • Author(s): Takase, K., Uenoyama, Y., Inoue, N., Matsui, H. Yamada, S., Shimizu, M. Homma, T., Tomikawa, J., Kanda, S., Matsumoto, H., Oka, Y., Tsukamura, H., and Maeda, K.-I.
  • Journal Title: Journal of Neuroendocrinology (in press)
  • Peer Reviewed
• [Journal Article] Identification of KiSS-1 product kisspeptin and steroid-sensitive sexually-dimorphic kisspeptin neurons in medaka(Oryzias latipes) (2008)
  • Author(s): Kanda, S., Akazome, Y., Yamamoto, N., Yamada, S., Tsukamura, H., Maeda, K., and Oka, Y.
  • Journal Title: Endocrinology 149 Pages: 2467-2476
  • Peer Reviewed
• [Journal Article] Coordinated synchronization in the electrically coupled network of terminal nerve gonadotropin-releasing hormone neurons as demonstrated by double patch-clamp study (2008)
  • Author(s): Haneda, K., and Oka, Y.
  • Journal Title: Endocrinology 149 Pages: 3540-3548
  • Peer Reviewed
• [Journal Article] Immunohistochemical localization of GnRH-like peptide in the brain of the cephalopod spear squid, Loligo bleekeri (2008)
  • Author(s): Amano, M., Oka, Y., Nagai, Y., Amiya, N., and Yamamori, K.
  • Journal Title: General and ComparatiVe Endocrinology 156 Pages: 277-284
  • Peer Reviewed
• [Journal Article] Identification and expression analysis of peroxisome proliferator-activated receptors cDNA in a reptile, the leopard gecko(Eublepharis macularius) (2008)
  • Author(s): Kato, K., Oka, Y., and Park, M.K.
  • Journal Title: Zoological Science 25 Pages: 492-502
  • Peer Reviewed
• [Presentation] Distribution of kisspeptin neurons in the CNS, with attention also to comparative aspects of Kissl/GPR54 system (2008)
  • Author(s): Vaudry, H., and Oka, Y.
  • Organizer: 1st world conference on kisspeptin signaling in the brain
  • Place of Presentation: Cordoba, Spain
  • Year and Date: 20081008-10
• [Presentation] Primary culture system of fish GnRH neurons for the cellular Physiological studies of effects of kisspeptin (2008)
  • Author(s): Abe, H., and Oka, Y.
  • Organizer: 1st world conference on kisspeptin signaling in the brain
  • Place of Presentation: Cordoba, Spain
  • Year and Date: 20081008-10
• [Presentation] Quantitative expression profiling of kissl and its receptor in the teleost medaka (2008)
  • Author(s): Okubo, K., Kanda, S., and Oka, Y.
  • Organizer: 1st world conference on kisspeptin signaling in the brain
  • Place of Presentation: Cordoba, Spain
  • Year and Date: 20081008-10
• [Presentation] Possible novel form of kisspeptin and kisspeptin receptors in the medaka, Oryzias latipes (2008)
  • Author(s): Akazome, Y., Kanda, S. Mitani, Y., Okubo, K., and Oka, Y.
  • Organizer: 1st world conference on kisspeptin signaling in the brain
  • Place of Presentation: Cordoba, Spain
  • Year and Date: 20081008-10
• [Presentation] Kisspeptin and steroid-sensitive sexually dimorphic kisspeptin neurons in medak(Oryzias latipes) (2008)
  • Author(s): Kanda, S., Akazome, Y. Yamamoto, N., Yamada, S. Tsukamura, H., Maeda, K., and Oka, Y.
  • Organizer: 1st world conference on kisspeptin signal ing in the brain
  • Place of Presentation: Cordoba, Spain
  • Year and Date: 20081008-10
• [Presentation] Three types of GnRH neurons and steroid-sensitive sexually dimorphic kisspeptin neurons in teleosts (2008)
  • Author(s): Oka, Y.
  • Organizer: US/Japan Neurosteroid Symposium 2008
  • Place of Presentation: Gifu
  • Year and Date: 20080908-11
• [Presentation] 終神経GnRHニューロンへのFMRFamideペプチドの過分極性作用に関する電気生理学的解析 (2008)
  • Author(s): 齊藤健、阿部秀樹、岡良隆
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 20080905-07
• [Presentation] 単一細胞電気穿孔による終神経GnRHニューロンへの外来分子導入法 (2008)
  • Author(s): 入江高行、尾崎令、阿部秀樹、岡良隆
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 20080905-07
• [Presentation] キンギョ嗅上皮におけるカルシウム振動 (2008)
  • Author(s): 河合喬文、阿部秀樹、岡良隆
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 20080905-07
• [Presentation] メダカ脳内kiss1遺伝子発現は卵巣エストロゲンにより調節され生殖状態を反映する (2008)
  • Author(s): 神田真司、赤染康久、松永拓也、山本直之、山田俊児、東村博子、前多敬一郎、岡良隆
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡
  • Year and Date: 20080905-07
• [Presentation] Functional analysis of the physiology of peptidergic systems using the transgenic medaka Neurobiology of,Animal Behavior and Communication (2008)
  • Author(s): Oka, Y.
  • Organizer: 31st Annual meeting of the Japan Neuroscience Society
  • Place of Presentation: Tokyo
  • Year and Date: 20080709-11
• [Presentation] GABAergic excitation in the terminal nerve GnRH neurons (2008)
  • Author(s): Nakane, R., and Oka, Y.
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 20080709-11
• [Presentation] Evolutionary aspects of peptidergic nervous systems in the vertebrate brain (2008)
  • Author(s): Oka, Y.
  • Organizer: International Seminar : Evolutionary Studies in Behavioral Neuroscience
  • Place of Presentation: Yokosuka
  • Year and Date: 20080625-27
• [Presentation] Electrophysiological and anatomical characterization of three distinct GnRH neuronal populations in the GFP transgenic medaka (2008)
  • Author(s): Kanda, S., Okubo, K., Isomae, S., Abe, H., Kobayashi, D., Takeda, H., Aida, K., Nagahama, Y., and Oka, Y.
  • Organizer: Annual Meeting Society for Neuroscience 2008
  • Place of Presentation: Washington DC, U.S.A
  • Year and Date: 2008-11-17
• [Presentation] Primary culture of the isolated terminal nerve-gonadotropin releasing hormone neurons derived from adult teleost brain and their electrophysiological properties (2008)
  • Author(s): Abe, H., and Oka, Y.
  • Organizer: Annual Meeting Society for Neuroscience 2008
  • Place of Presentation: Washington DC, U.S.A
  • Year and Date: 2008-11-17
• [Presentation] Calcium oscillation of the olfactory non-sensory cells in the goldfish (2008)
  • Author(s): Kawai, T., Abe, H., and Oka, Y.
  • Organizer: Annual Meeting Society for Neuroscience 2008
  • Place of Presentation: Washington DC, U.S.A
  • Year and Date: 2008-11-17
• [Presentation] Coordinated synchronization in the electrically coupled network of terminal nerve GnRH(gonadotropin-releasing hormone) neurons as demonstrated by double patch-clamp study (2008)
  • Author(s): Oka, Y., and Haneda, K.
  • Organizer: Annual Meeting Society for Neuroscience 2008
  • Place of Presentation: Washington DC, U.S.A
  • Year and Date: 2008-11-17
• [Presentation] 脊椎動物に共通する生殖の中枢制御のしくみをメダカの脳から知る (2008)
  • Author(s): 岡良隆
  • Organizer: 第101回日本繁殖生物学会シンポジウム「新規生理活性因子メタスチン」
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-20
• [Presentation] Medaka brain teaches us the mechanisms of the central regulation of reproduction common to the vertebrate species (2008)
  • Author(s): Oka, Y.
  • Organizer: 第35回日本神経内分泌学会・第23回日本下垂体研究会シンポジウム「視床下部・下垂体調節機構の新展開」
  • Place of Presentation: 東京
  • Year and Date: 2008-08-28
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/naibunpi/Oka/Oka_lab_publications.html