Optical analysis of functional formation of the brainstem neural circuit and its r egulation by the depolarization wave

2009 Fiscal Year Final Research Report

• Project/Area Number: 20021013
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: SATO Katsushige Tokyo Medical and Dental University, 人間健康学部, 教授 (80291342)
• Co-Investigator(Renkei-kenkyūsha): SATO Yoko 関東学院大学, 人間環境学部, 教授 (70251501)
• Project Period (FY): 2008 – 2009
• Keywords: depolarization wave / 光学計測 / 膜電位感受性色素 / embryo / gap junction / 脳幹 / 中枢神経系 / 神経回路網

Research Abstract

In the developing central nervous system (CNS), immature neural networks generate spontaneous correlated activity in a wide range. Using the optical imaging technique with voltage-sensitive dyes, we found a widely-spreading wave in the rat and chick embryos, which was termed the depolarization wave. One novelty of the depolarization wave was that it traveled over a wide region of the CNS including the brainstem, spinal cord, cerebellum, and part of the cerebrum. Another outstanding feature was that it was non-specifically triggered by various types of sensory inputs as well as endogenous spontaneous activity. These profiles suggest that the wave may not serve as a simple regulator of specific neuronal circuit formation, but might play a more global role in CNS development. The depolarization wave was mediated by multiple neurotransmitters and gap junctions, and the dominant transmitter switched from acetylcholine to glutamate with development. Surveillance of the origin of the depolarization wave showed that the wave initially originated in the upper cervical cord/lower medulla near the obex and was generated by multiple regions at the later stages. This network possibly works as a self-distribution system that maintains the correlated activity in the developing CNS.

Research Products

• [Journal Article] Demonstration of a neural circuit critical for imprinting behavior in chicks. (2010)
  • Author(s): Nakamori, T., Sato, K., Atoji, Y., Kanamatsu T., Tanaka, K., Ohki-Hamazaki, H.
  • Journal Title: Journal of Neuroscience 30 Pages: 4467-4480
• [Journal Article] Origin of the earliest correlated neuronal activity in the chick embryo revealed by optical imaging with voltage-sensitive dyes. (2009)
  • Author(s): Momose-Sato, Y., Mochida, H., Kinoshita, M.
  • Journal Title: European Journal of Neuroscience 29 Pages: 1-13
• [Journal Article] Switching of the transmitters that mediate hindbrain correlated activity in the chick embryo. (2009)
  • Author(s): Mochida, H., Sato, K., Momose-Sato, Y.
  • Journal Title: European Journal of Neuroscience 29 Pages: 14-30
• [Journal Article] Optical analysis of neural circuit formation in the embryonic brain. (2008)
  • Author(s): Sato. K., Momose-Sato, Y.
  • Journal Title: Clinical and Experimental Pharmacology and Physiology 35 Pages: 706-713
• [Journal Article] Using voltage-sensitive dye recording to image the functional development of neuronal circuits in vertebrate embryos. (2008)
  • Author(s): Glover, J.C., Sato, K., Momose-Sato, Y.
  • Journal Title: Developmental Neurobiology 68 Pages: 804-816
• [Journal Article] Processing of sting pheromone and its components in the antennal lobe of the worker honeybee. (2008)
  • Author(s): Wang, S., Sato, K., Giurfa, M., Zhang, S.
  • Journal Title: Journal of Insect physiology 54 Pages: 833-841
• [Presentation] Spontaneous depolarization waves in the mouse embryo revealed by optical imaging with a voltage-sensitive dye. (2009)
  • Author(s): Momose-Sato, Y., Sato, K.
  • Organizer: Society for Neuroscience 39th Annual Meeting
  • Place of Presentation: Chicago, USA
  • Year and Date: 20091017-20091021
• [Presentation] Spontaneous depolarization wave revealed by voltage-sensitive dye imaging. (2009)
  • Author(s): Sato, K., Momose-Sato, Y.
  • Organizer: 3rd Viktor Hamburger Symposium on Motor System Development "Development of Motor Circuits: At the Crossroads of Genes and Activity"
  • Place of Presentation: Renaissance Hotel, Chicago, USA
  • Year and Date: 20091015-20091016
• [Presentation] Functional development of auditory and vestibular neural circuits in the embryonic chick brainstem revealed by voltage-sensitive dye imaging. (2009)
  • Author(s): Sato, K., Glover, J.C., Momose-Sato, Y.
  • Organizer: XXXVI International Congress of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20090727-20090801
• [Presentation] Optical imaging of large-scale depolarization waves in the developing CNS. (2009)
  • Author(s): Momose-Sato, Y., Sato, K.
  • Organizer: XXXVI International Congress of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20090727-20090801
• [Presentation] A telencephalic neural circuit critical for visual imprinting in chicks. (2009)
  • Author(s): Nakamori, T., Sato, K., Tanaka, K., Ohki-Hamazaki H.
  • Organizer: XXXVI International Congress of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20090727-20090801
• [Presentation] Optical imaging of neuronal activities: From embryos to human brain. (2009)
  • Author(s): Sato, K., Momose-Sato, Y.
  • Organizer: The 4th Asian and Pacific Rim symposium on biophotonics
  • Place of Presentation: Jeju island, South Korea
  • Year and Date: 20090527-20090529
• [Presentation] A telencephalic neural circuit critical for visual imprinting in chicks. (2009)
  • Author(s): Nakamori, T., Sato, K., Tanaka, K., Ohki-Hamazaki, H.
  • Organizer: Journal of Physiological Sciences
  • Year and Date: 20090000
• [Presentation] Functional development of the N. IX and N. X-related nuclei in the embryonic rat brainstem: Optical mapping with voltage-sensitive dyes. (2009)
  • Author(s): Sato, K., Momose-Sato, Y.
  • Organizer: Neuroscience Research
  • Year and Date: 20090000
• [Presentation] Switching of the transmitters that mediate the depolarization wave in the embryonic chick CNS. (2009)
  • Author(s): Momose-Sato, Y., Mochida, H., Sato, K.
  • Organizer: Neuroscience Research
  • Year and Date: 20090000
• [Presentation] Activation of a telencephalic circuit is critical for visual imprinting in chicks. (2009)
  • Author(s): Nakamori, T., Sato, K., Tanaka, K., Ohki-Hamazaki, H.
  • Organizer: Neuroscience Research
  • Year and Date: 20090000
• [Presentation] Application of intraoperative intrinsic optical imaging in epilepsy surgery. (2008)
  • Author(s): Maehara, T., Inaji, M., Nariai, T., Sato, K., Ohno, K.
  • Organizer: American Epilepsy Society 62nd Annual Meeting
  • Place of Presentation: Seattle, USA
  • Year and Date: 20081205-20081209
• [Presentation] Functional organization of the glossopharyngeal and vagus nerve-related nuclei in the embryonic rat brainstem: Optical mapping with voltage-sensitive dyes. (2008)
  • Author(s): Sato, K., Momose-Sato, Y.
  • Organizer: Society for Neuroscience 38th Annual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20081115-20081119
• [Presentation] Switching of the transmitters that mediate hindbrain correlated activity in the chick embryo. (2008)
  • Author(s): Momose-Sato, Y., Mochida, H., Sato, K.
  • Organizer: Society for Neuroscience 38th Annual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20081115-20081119
• [Presentation] Neuronal excitability to generate spontaneous activity in the medulla and spinal cord of the chick embryo. (2008)
  • Author(s): Kinoshita, M., Momose-Sato, Y., Sato, K.
  • Organizer: Society for Neuroscience 38th Annual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20081115-20081119
• [Presentation] Development of vagal afferent projections circumflex to the obex in the embryonic chick brainstem: optical imaging with voltage-sensitive dyes. (2008)
  • Author(s): Sato, K., Kinoshita, M., Momose-Sato, Y.
  • Organizer: Journal of Physiological Sciences
  • Year and Date: 20080000
• [Presentation] Origin of the depolarization wave at an initial stage of wave expression revealed by optical imaging. (2008)
  • Author(s): Momose-Sato, Y., Mochida, H., Kinoshita, M., Sato, K.
  • Organizer: Journal of Physiological Sciences
  • Year and Date: 20080000
• [Presentation] Optical approaches to functional neural circuit formation in the embryonic brainstem. (2008)
  • Author(s): Sato, K.
  • Organizer: Journal of Physiological Sciences
  • Year and Date: 20080000
• [Presentation] Optical recording of vagal afferent projections circumflex to the obex in the embryonic chick brainstem. (2008)
  • Author(s): Sato, K., Kinoshita, M., Momose-Sato, Y.
  • Organizer: Neuroscience Research
  • Year and Date: 20080000
• [Presentation] Origin of the correlated wave activity at an initial stage of wave expression revealed by optical imaging. (2008)
  • Author(s): Momose-Sato, Y., Mochida, H., Kinoshita, M., Sato, K.
  • Organizer: Neuroscience Research
  • Year and Date: 20080000
• [Presentation] Regional differences in neuronal excitability of the hindbrain in chick embryo. (2008)
  • Author(s): Kinoshita, M., Momose-Sato, Y., Sato, K.
  • Organizer: Neuroscience Research
  • Year and Date: 20080000
• [Presentation] Functional development of auditory and vestibular neural circuits in the embryonic chick brainstem revealed by voltage-sensitive dye imaging. (2008)
  • Author(s): Sato, K., Glover, J.C., Momose-Sato, Y.
  • Organizer: Journal of Physiological Sciences
  • Year and Date: 20080000
• [Presentation] Optical imaging of large-scale depolarization waves in the developing CNS. (2008)
  • Author(s): Momose-Sato, Y., Sato, K.
  • Organizer: Journal of Physiological Sciences
  • Year and Date: 20080000
• [Presentation] Regional gradient of neuronal excitability to generate spontaneous activity in the hindbrain of the chick embryo. (2006)
  • Author(s): Kinoshita, M., Momose-Sato, Y., Sato, K.
  • Organizer: 6th FENS Forum of European Neuroscience
  • Year and Date: 20060000
• [Book] The Voltage Imaging Book.(Eds. Canepari, M. & Zecevic, D.)
  • Author(s): Momose-Sato, Y., Sato, K., Kamino, K.
  • Publisher: Springer(in press)
• [Remarks]
  • URL: http://square.umin.ac.jp/optical/