Elucidation of the physiological role of vimentin and desmin by artificial glycopolymers

2010 Fiscal Year Final Research Report

• Project/Area Number: 20200016
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science; Cell biology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: ISE Hirohiko Tokyo Institute of Technology, フロンティア研究機構, 特任講師 (10324253)
• Project Period (FY): 2008 – 2010
• Keywords: バイオマテリアル / 細胞骨格 / レクチン / ビメンチン

Research Abstract

In this study, we found that vimentin and desmin, cytoskeletal proteins, possess N-aceylglucosamine (GlcNAc)-binding lectin-like properties on cell surfaces. It was found that these molecules were localized to cells surface by the tetrameric formation and the rod II domains that are GlcNAc-binding site were exposed to cell surfaces.

Research Products

• [Journal Article] Gene delivery system based on highly specific recognition of surface-vimentin with N-acetylglucosamine immobilized polyethylenimine. (2011)
  • Author(s): Kim SJ, Ise H (Corresponding author), Goto M, Komura K, Cho CS, Akaike T.
  • Journal Title: Biomaterials 32 Pages: 3471-3480
• [Journal Article] Vimentin and desmin possess GlcNAc-binding lectin-like properties on cell surfaces. (2010)
  • Author(s): Ise H (Corresponding author), Kobayashi S, Goto M, Sato T, Kawakubo M, Takahashi M, Ikeda U, Akaike T.
  • Journal Title: Glycobiology. 20 Pages: 843-864
• [Journal Article] Surface coating of bone marrow cells with N-acetylglucosamine for bone marrow implantation therapy. (2009)
  • Author(s): Kobayashi S, Ise H (Corresponding author), Takahashi M, Goto M, Akaike T, Ikeda U.
  • Journal Title: Biomaterials. 30 Pages: 574-582
• [Journal Article] Potential feasibility of early bone marrow cell injection into the spleen for creating functional hepatocytes. (2009)
  • Author(s): Misawa R, Soeda J, Ise H, Takahashi M, Kubota K, Mita A, Nakata T, Miyagawa S.
  • Journal Title: Transplantation. 87 Pages: 1147-1154
• [Journal Article] Bone marrow CXCR4 induction by cultivation enhances therapeutic angiogenesis. (2009)
  • Author(s): Shiba Y, Takahashi M, Hata T, Murayama H, Morimoto H, Ise H, Nagasawa T, Ikeda U.
  • Journal Title: Cardiovasc Res. 81 Pages: 169-177
• [Journal Article] Deficiency of tumour necrosis factor-alpha and interferon-gamma in bone marrow cells synergistically inhibits neointimal formation following vascular injury. (2008)
  • Author(s): Murayama H, Takahashi M, Takamoto M, Shiba Y, Ise H, Koyama J, Tagawa Y, Iwakura Y, Ikeda U.
  • Journal Title: Cardiovasc Res. 80 Pages: 175-180
• [Journal Article] Critical role of bone marrow-derived ASC, an inflammasome adaptor molecule, in neointimal formation after vascular injury in mice. (2008)
  • Author(s): Yajima N, Takahashi M, Morimoto H, Shiba Y, Takahashi Y, Masumoto J, Ise H, J Sagara, Nakayama J, Taniguchi S, Ikeda U.
  • Journal Title: Circulation. 117 Pages: 3079-3087
• [Journal Article] MCP-1 induces cardioprotection against ischemia/reperfusion injury: Role of reactive oxygen species. (2008)
  • Author(s): Morimoto H, Hirose M, Takahashi M, Kawaguchi M, Ise H, Kolattukudy PE, Yamada M, Ikeda U.
  • Journal Title: Cardiovasc Res. 78 Pages: 554-562
• [Journal Article] Embryonic undifferentiated cells show scattering activity on a surface coated with immobilized E-cadherin. (2008)
  • Author(s): Nagaoka M, Ise H, Harada I, Koshimizu U, Maruyama A, Akaike T.
  • Journal Title: J Cell Biochem. 103 Pages: 296-310
• [Journal Article] Bone Marrow-derived Cells Fuse with Hepatic Oval Cells but are not involved in Hepatic Tumorigenesis in the Choline-deficient Ethionine-supplemented Diet Rat Model. (2008)
  • Author(s): Kubota K, Soeda J, Misawa R, Mihara M, Miwa S, Ise H, Takahashi M, Miyagawa S.
  • Journal Title: Carcinogenesis. 29 Pages: 448-454
• [Journal Article] Modulation of mucus production by interleukin-13 receptor alpha in the human airway epithelium. (2008)
  • Author(s): Tanabe T, Fujimoto K, Yasuo M, Tsushima K, Yoshida K, Ise H, Yamaya M.
  • Journal Title: Clin Exp Allergy. 38 Pages: 122-134
• [Presentation] N-acetylglucosamine-binding activity of vimentin found by artificial biomimicking Glycopolymers (2011)
  • Author(s): IseH, et al
  • Organizer: ICBS2011
  • Place of Presentation: Tsukuba
  • Year and Date: 2011-03-17
• [Presentation] N-アセチルグルコサミン結合活性に基づくビメンチンの役割の解明 (2010)
  • Author(s): 伊勢裕彦, 他
  • Organizer: BMB2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] N-アセチルグルコサミン結合活性含有糖鎖高分子PV-GlcNAcに見出された細胞骨格分子Vimentinのレクチン活性の解明 (2010)
  • Author(s): 伊勢裕彦, 他
  • Organizer: 第32回日本バイオマテリアル学会
  • Place of Presentation: 広島
  • Year and Date: 2010-11-29
• [Presentation] Vimentin and desmin possess GlcNAc-binding lectin-like properties on cell surfaces (2010)
  • Author(s): IseH, et al
  • Organizer: 25th International Carbohydrate Symposium
  • Place of Presentation: Tokyo
  • Year and Date: 2010-08-03
• [Presentation] ビメンチンの細胞外出現とN-アセチルグルコサミン結合活性の役割 (2010)
  • Author(s): 伊勢裕彦, 他
  • Organizer: 第62回細胞生物学会大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-19
• [Presentation] Development of a drug delivery system using N-acetylglucosamine-conjugated liposomes (2009)
  • Author(s): IseH, Goto M, Akaike T
  • Organizer: The 7th Asia 3 (China-Japan-Korea) Foresight Symposium on Gene Therapy and Biomaterials
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2009-05-25
• [Presentation] Development of liver regenerative therapy using glycoside-modified bone marrow cells (2009)
  • Author(s): Ise H, Akaike T
  • Organizer: The Okayama 2009 Joint Conference of the 10th biannual Cell Transplant Society Congress
  • Place of Presentation: Okayama
  • Year and Date: 2009-04-20
• [Presentation] Novel cell delivery system of bone marrow cells for treatment of myocardial infarction by coating with N-acetylglucosamine on the cell surface (2009)
  • Author(s): Ise H, Kobayashi S, Goto M, Akaike T
  • Organizer: The Okayama 2009 Joint Conference of the 10th biannual Cell Transplant Society Congress
  • Place of Presentation: Okayama
  • Year and Date: 2009-04-20
• [Book] 11医療に向けた細胞認識機能性バイオマテリアル(バイオ研究のフロンティア3医療・診断をめざす先端バイオテクノロジー)(関根光雄/編)
  • Author(s): 伊勢裕彦、赤池敏宏
  • Total Pages: 98-109
  • Publisher: 工学図書株式会社