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2010 Fiscal Year Final Research Report

Development of DNA Sequencing Technologies Using Gating Nanopores

Research Project

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Project/Area Number 20200025
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

Allocation TypeSingle-year Grants
Research Field Nanomaterials/Nanobioscience
Analytical chemistry
Research InstitutionOsaka University

Principal Investigator

TANIGUCHI Masateru  Osaka University, 産業科学研究所, 准教授 (40362628)

Project Period (FY) 2008 – 2010
Keywordsナノボア / ゲーティングナノボア / DNAシーケンサー / 微細加工 / 単一分子計測 / トンネル電流 / イオン電流
Research Abstract

Gating nanopores are the key devices for third generation DNA sequencing technologies. These nanodevices will make sequencing kilobase length single-stranded genomic DNA or RNA or identifying individual small molecules using only electric currents and without fluorescent labels at low cost and unheard speeds. We have developed vertical and parallel gating nanopores with embedded nanogap-electrodes in a solid-state nanopore. The vertical type consists of a single nanogap electrode with the nanopore perpendicular to the surface of the silicon substrate. The parallel type consists of a single nanogap electrode with the nanopore parallel to the surface of the substrate. We synthesized vertical gating nanopores with a diameter of 30 nm using an 11-step nanofabrication process. Vertical gating nanopores can indetify a single Au nanoparticle (~ = 28 nm) passing through them by changes in the electric current flowing between the nano-electrodes. Single base molecules of DNA can be identified by changes in tunneling current between nano-electrodes using parallel gating nanopores, incorporating a microfluidic channel in to nano-fabricated mechanically controllable break junction. We found that single-molecule electrical conductance order thymine < cytosine < adenine < guanine corresponds to the highest occupied molecular orbital (HOMO) energy order.

  • Research Products

    (12 results)

All 2011 2010 2009 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (7 results) Remarks (1 results)

  • [Journal Article] Single Molecule Identification via Electric Current Noise2011

    • Author(s)
      Makusu Tsutsui, Masateru Taniguchi, Tomoji Kawai
    • Journal Title

      Nature Communications 1

      Pages: 138-142

    • Peer Reviewed
  • [Journal Article] dentifying Single Nucleotides by Tunneling Current2010

    • Author(s)
      Makusu Tsutsui, Masateru Taniguchi, Kazumichi Yokota, Tomoji Kawai
    • Journal Title

      Nature Nanotechnology 5

      Pages: 286-290

    • Peer Reviewed
  • [Journal Article] Fabrication of the Gating Nanopore, Appl.2009

    • Author(s)
      Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai
    • Journal Title

      Phys.Lett. 95

      Pages: 123701-123703

    • Peer Reviewed
  • [Journal Article] Atomistic Mechanics and Formation Mechanism of Metal-Molecule-Metal Junctions2009

    • Author(s)
      Makusu Tsutsui, Masateru Taniguchi, Tomoji Kawai
    • Journal Title

      Nano Left. 9

      Pages: 2433-2439

    • Peer Reviewed
  • [Presentation] 核酸塩基分子の単分子識別-次々世代DNAシーケンサーにむけて-2011

    • Author(s)
      谷口正輝
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学(神奈川県)
    • Year and Date
      2011-03-28
  • [Presentation] 1分子解析技術による次々世代DNAシーケンサーの開発2011

    • Author(s)
      谷口正輝
    • Organizer
      日本表面科学会第69回表面科学研究会
    • Place of Presentation
      東京工業大学(東京都)
    • Year and Date
      2011-03-09
  • [Presentation] Development of Gating Nanopores for Single-Molecule Electrical Sequencing2011

    • Author(s)
      Masateru Taniguchi
    • Organizer
      International Symposium : Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules
    • Place of Presentation
      京都国際会館(京都府)
    • Year and Date
      2011-01-24
  • [Presentation] Development of 3 D Generation DNA Sequencer Using Gating Nanopore Devices2010

    • Author(s)
      Masateru Taniguchi
    • Organizer
      2nd Japanese-Russian Young Scientists Conference on Nano-materials and Nano-technology
    • Place of Presentation
      東京工業大学(東京都)
    • Year and Date
      2010-09-22
  • [Presentation] ゲーティングナノボアを用いた1分子検出2010

    • Author(s)
      谷口正輝
    • Organizer
      バイオ・マイクロシステム研究会、電気学会研究会
    • Place of Presentation
      名古屋大学(愛知県)
    • Year and Date
      2010-01-29
  • [Presentation] Identification of Single Nucleotides Using Gating Nanopores2010

    • Author(s)
      Masateru Taniguchi
    • Organizer
      13th SANKEN International Symposium 2010
    • Place of Presentation
      関西空港ANAホテル(大阪府)
    • Year and Date
      2010-01-19
  • [Presentation] ゲーティング固体ナノボアを用いた電気計測2009

    • Author(s)
      谷口正輝
    • Organizer
      第56回応用物理学関係連合講演会(招待講演)
    • Place of Presentation
      筑波大学(茨城県)
    • Year and Date
      2009-03-31
  • [Remarks] ホームページ

    • URL

      http://www-kawai.sanken.osaka-u.ac.jp/

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Published: 2012-02-13   Modified: 2016-04-21  

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