Reconstitution of photosynthetic reactions of the phototrophic proteobacteria in E. coli

2010 Fiscal Year Final Research Report

• Project/Area Number: 20200030
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: 基礎ゲノム科学; Plant molecular biology/Plant physiology
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: KATO Junnichi Tokyo Metropolitan University, 大学院・理工学研究科, 准教授 (10194820)
• Co-Investigator(Kenkyū-buntansha): NAGASHIMA Kennji 首都大学東京, 大学院・理工学研究科, 准教授 (80264589)
• Project Period (FY): 2008 – 2010
• Keywords: 微生物ゲノム / 色素体機能・光合成 / 光合成細菌

Research Abstract

We developed a sequential cloning system for cloning and expression of the large gene cluster. Using this system we cloned and introduced the photosysnthesis gene cluster of photosynthetic bacteria into the E. coli chromosome and plasmid. We succeeded in reconstitution of the biosynthesis of some carotenoids and of an intermediate of the bacteriochlorophyll, chlorophyllide a.

Research Products

• [Journal Article] The cytochrome c8 involved in the nitrite reduction pathway acts also as electron donor to the photosynthetic reaction center in Rubrivivax gelatinosus. (2011)
  • Author(s): Nagashima S、 Shimada K、 Vermeglio A、 Nagashima KVP
  • Journal Title: Biochim.Biophys.Acta. 1807(2) Pages: 189-196
• [Journal Article] Cytochrome c4 can be involved in the photosynthetic electron transfer system in the purple bacterium (2009)
  • Author(s): Ohmine M, Matsuura K, Shimada K, Alric J, Vermeglio A, Nagashima KVP
  • Journal Title: Rubrivivax gelatinosus. Biochemistry 48(38) Pages: 9132-9139
• [Journal Article] Characterization of a blue copper protein, auracyanin, of the filamentous anoxygenic phototrophic bacterium, Roseiflexus castenholzii. (2009)
  • Author(s): Tsukatani Y, Nakayama N, Shimada K, Mino H, Itoh S, Matsuura K, Hanada S, Nagashima KVP
  • Journal Title: Arch.Biochem.Biophys. 490(1) Pages: 57-62
• [Presentation] 大腸菌における光合成機能の再構成 (2011)
  • Author(s): 藤原弘平、城本史寛、岩本明、本多弘典、篠田卓弥、永島賢治、加藤潤一
  • Organizer: 第5回日本ゲノム微生物学会年会
  • Place of Presentation: 仙台
  • Year and Date: 20110300
• [Presentation] 光合成のアンテナ系-反応中心複合体のITO基板上への組織化と光電流応答 (2011)
  • Author(s): 近藤政晴、原田香織、永島咲子、永島賢治、橋本秀樹、出羽毅久、南後守
  • Organizer: 第91回日本化学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20110300
• [Presentation] 光合成で駆動する新しい生物代謝:細菌への遺伝子操作による試み (2011)
  • Author(s): 永島賢治
  • Organizer: 第91回日本化学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20110300
• [Presentation] An attempt to reconstitute photosynthetic reactions of the phototrophic proteobacterium (2010)
  • Author(s): Akira Iwamoto, Hirofumi Honda, Kohei Fujiwara, Takumi Eguchi, Kenji V.P.Nagashima, Jun-ichi Kato.
  • Organizer: R.sphaeroides, in E.coli.第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2010-12-11
• [Presentation] 大腸菌をプラットフォームにした光合成機能の再構成 (2010)
  • Author(s): 城本史寛、岩本明、本多弘典、藤原弘平、篠田卓弥、島原佑基、永島賢治、加藤潤一
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] 紅色光合成細菌の光化学反応中心結合型チトクロムcに含まれる低電位ヘムの機能解明のための実験系の確立 (2010)
  • Author(s): 中山陽介、嶋田敬三、永島賢治
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-18
• [Presentation] 大腸菌をプラットフォームにした光合成機能の再構成 (2010)
  • Author(s): 岩本明、本多弘典、藤原弘平、江口拓未、永島賢治、加藤潤一
  • Organizer: 第4回日本ゲノム微生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2010-03-07
• [Presentation] 大腸菌ゲノムの縮小化と必須遺伝子群の機能解析及び他種ゲノムの導入と機能の再構成 (2009)
  • Author(s): 加藤潤一
  • Organizer: 「細胞を創る」研究会 2.0
  • Place of Presentation: 東京
  • Year and Date: 2009-10-03
• [Remarks] ホームページ等