Development of genetically engineered animals by use of non-B DNA structure for highly and stably expression of transgene

2010 Fiscal Year Final Research Report

• Project/Area Number: 20200032
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Applied animal science; Applied genomics
• Research Institution: Kinki University
• Principal Investigator: MITANI Tasuku Kinki University, 先端技術総合研究所, 准教授 (10322265)
• Co-Investigator(Kenkyū-buntansha): ANZAI Masayuki 近畿大学, 先端技術総合研究所, 准教授 (30454630)
• Co-Investigator(Renkei-kenkyūsha): OHYAMA Takashi 早稲田大学, 教育・総合科学学術院, 教授 (60268513)
• Project Period (FY): 2008 – 2010

Research Abstract

This study examined the function of non-B DNA sequence on the activation of transcription by RNAPII and/or RNAPIII to develop transgenic animals with highly efficient and stable expression of transgene. The results may be summarized as follows : T20 (180bp artificial curved DNA) as a model of non-B DNA sequence showed (1) the activation of the transcription by RNAPII in mouse ES cells, (2) the stability of transcription and chromatin dynamics during in vitro differentiation of ES cells, and (3) the possibility of the production of T20-driven transgenic mice.

Research Products

• [Journal Article] Competence of an artificial bent DNA as a transcriptional activator in the mouse ES cells. (2011)
  • Author(s): Tanase J, Mitani T, Udagawa K, Nishikawa J, Takashi Ohyama T.
  • Journal Title: Mol.Biol.Reports 38 Pages: 37-47
  • Peer Reviewed
• [Journal Article] In vivo application of an RNAi strategy for the selective suppression of a mutant allele. (2011)
  • Author(s): Kubodera T, Yamada H, Anzai M, Ohira S, Yokota S, Hirai Y, Mochizuki H, Shimada T, Mitani T, Mizusawa H, Yokota T.
  • Journal Title: Human Gene Therapy 22 Pages: 27-34
  • Peer Reviewed
• [Journal Article] Differentiation diversity of mouse parthenogenetic embryonic stem cells in chimeric mice. (2010)
  • Author(s): Onodera Y, Teramura T, Ozawa M, Takehara T, Mitani T, Anzai M, Sagawa N, Hamanishi C, Hosoi Y, Fukuda K.
  • Journal Title: Theriogenology 74 Pages: 135-145
  • Peer Reviewed
• [Journal Article] Silencing efficency differs among tissues and endogenous micro RNA pathway in preserved in short hairpin RNA transgenic mice. (2009)
  • Author(s): Sasaguri H, Mitani T, Anzai M, Kubodera T, Saito Y, Yamada H, Mizusawa H, Yokota T.
  • Journal Title: FEBS Letters 583 Pages: 213-218
  • Peer Reviewed
• [Journal Article] マウス体細胞核移植由来再構築卵子における核内構造制御タンパク質の発現の解析 (2009)
  • Author(s): 西山有依, 森田真裕, 安齋政幸, 加藤博己, 細井美彦, 三谷匡, 入谷明
  • Journal Title: Mem.Inst.Adv.Technol., Kinki Univ. 14 Pages: 21-30
• [Presentation] マウス胚性幹細胞の分化誘導過程における細胞核染色体テリトリーと遺伝子座の動態 (2010)
  • Author(s): 西山有依, 川口翔, 平野大起, 森木甲子郎, 藤本佑希, 細井美彦, 田辺秀之, 三谷匡
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-20101210
• [Presentation] マウス胚性幹細胞の分化過程における細胞核染色体テリトリーと遺伝子座の動態 (2010)
  • Author(s): 西山有依, 森木甲子郎, 藤本佑希, 安齋政幸, 加藤博己, 松本和也, 佐伯和弘, 入谷明, 細井美彦, 田辺秀之, 三谷匡
  • Organizer: 第28回日本受精着床学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20100727-20100729
• [Presentation] Expression of the components of chromatin remodeling factor SWR1 complex in mouse somatic nuclear transfer eggs. (2009)
  • Author(s): 西山有依, 森木甲子郎, 森田真裕, 安齋政幸, 加藤博己, 細井美彦, 入谷明, 原田昌彦, 三谷匡
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] Dynamics of Arp family proteins and components of chromatin remodeling complexes in in vitro differentiation of mouse embryonic stem cells. (2009)
  • Author(s): 森木甲子郎, 西山有依, 川村紘子, 安齋政幸, 加藤博己, 細井美彦, 原田昌彦, 入谷明, 三谷匡
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] Stable expression of transgenes realized by chromatin engineering. (2009)
  • Author(s): 棚瀬潤一, 宇田紘司, 西川純一, 三谷匡, 大山隆
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091200
• [Presentation] マウスES細胞の未分化維持機構においてABCトランスポーターBcrp1が与える影響 (2009)
  • Author(s): 川村紘子, 川合智子, 田口善智, 安齋政幸, 加藤博己, 細井美彦, 三谷匡, 入谷明
  • Organizer: 第102回日本繁殖生物学会大会
  • Place of Presentation: 奈良
  • Year and Date: 20090910-20090912
• [Presentation] マウス体細胞核移植由来卵子におけるクロマチンリモデリング複合体、SWR1複合体の構成因子の発現 (2009)
  • Author(s): 西山有依, 森田真裕, 安齋政幸, 加藤博己, 細井美彦, 原田昌彦, 三谷匡, 入谷明
  • Organizer: 第102回日本繁殖生物学会大会
  • Place of Presentation: 奈良
  • Year and Date: 2009-09-10
• [Presentation] マウスES細胞分化誘導過程におけるにABCトランスポーターBcrp1アイソフォームの発現様式およびRNAiによるアイソフォームAノックダウンES細胞樹立の試み (2008)
  • Author(s): 川村紘子, 網本直記, 田口善智, 安齋政幸, 加藤博己, 細井美彦, 入谷明, 三谷匡
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 20081209-20081212
• [Presentation] マウスES細胞の分化誘導過程におけるにABCトランスポーターBcrp1アイソフォームの発現様式 (2008)
  • Author(s): 川村紘子, 網本直記, 田口善智, 安齋政幸, 加藤博己, 細井美彦, 入谷明, 三谷匡
  • Organizer: 第26回日本受精着床学会総会
  • Place of Presentation: 福岡
  • Year and Date: 20080828-20080829
• [Presentation] Differentiation of mouse ES cells to hepatocytes and its application. (2008)
  • Author(s): Mitani T.
  • Organizer: Recent Advance of Embryonic and Somatic Stem Cells in Biomedical Science Workshop.
  • Place of Presentation: Bangkok, Thailand (Invited Speaker)
  • Year and Date: 20080728-20080729
• [Presentation] Expression of Bcrp1 mRNA isoforms during in vitro differentiation of mouse embryonic stem cells. (2008)
  • Author(s): Kawamura H, Amimoto N, Moriki K, Morita M, Anzai M, Kato H, Hosoi Y, Iritani A, Mitani T.
  • Organizer: 41st Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Tokushima, Japan.
  • Year and Date: 20080528-20080530
• [Remarks] ホームページ等
  • URL: http://rais.itp.kindai.ac.jp/researchdb/