2010 Fiscal Year Final Research Report
Generation of MicroAntibody by directed evolution
| Project/Area Number |
20200033
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
Living organism molecular science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
TSUMURAYA Takeshi Osaka Prefecture University, 理学系研究科, 准教授 (00372855)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJII Ikuo 大阪府立大学, 理学系研究科, 教授 (70189984)
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| Project Period (FY) |
2008 – 2010
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| Keywords | ペプチド / コンビナトリアル化学 / ポストゲノム創薬 / マイクロ抗体 / 分子プローブ / 分子標的医薬 / ライブラリー |
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| Research Abstract |
In this study, we have developed a method to downsize the antibody by the combination of structure-design of proteins and directed evolution. We have constructed a library of alternative-binding peptide with non-immunoglobulin domain to develop molecular probe and molecular targeting drug. The helix-loop-helix peptide, which binds to specifically target molecules, is named as "MicroAntibody". We have constructed a library of MicroAntibody to generate human IgG-Fc-specific peptides and Aurora kinase-specific peptides. We also studied immunogenicity and stability of MicroAntibody
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[Journal Article] Critical Contribution of Aromatic Rings to Specific Recognition of Polyether Rings by Antibody : The Case of Ciguatoxin CTX3C and Its Specific Antibody 1C92008
Author(s)
K.Tsumoto, A.Yokota, Y.Tanaka, M.Ui, T.Tsumuraya, I.Fujii, I.Kumagai, Y.Nagumo, H.Oguri, M.Inoue, M.Hirama
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Journal Title
J.Mol.Biol. 283
Pages: 12259-12266
Peer Reviewed
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[Presentation]2009
Author(s)
R.El-Haggar, Z.Ye, Y.Ishino, K.Kamikawa, T.Tsumuraya, I.Fujii
Organizer
2^<nd> Switzerland-Japan Biomolecular Chemistry Symposium
Place of Presentation
東京大学(東京都)
Year and Date
2009-09-11
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