• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2010 Fiscal Year Final Research Report

Molecular targeted drug discovery for cancer cell radiosensitization using RNA aptamars

Research Project

  • PDF
Project/Area Number 20200039
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

Allocation TypeSingle-year Grants
Research Field Radiation science
Living organism molecular science
Research InstitutionTottori University

Principal Investigator

KURIMASA Akihiro  Tottori University, 大学院・医学系研究科, 准教授 (80343276)

Co-Investigator(Kenkyū-buntansha) NIWA Ohtaura  放射線医学総合研究所, 重粒子医科学センター, 副センター長 (80093293)
OKAYASU Ryuichi  放射線医学総合研究所, 重粒子医科学センター, グループリーダー (50356135)
Project Period (FY) 2008 – 2010
Keywords放射線治療生物学 / 分子標的創薬 / DNA修復 / RNA工学
Research Abstract

It is well known that blocking protein kinases related to DNA repair machinery such as DNA-PKcs, ATM, or ATR enhance sensitivity to ionizing radiation (IR). From this point of view, we expect that tumor cell sensitivity against radiotherapy is enhanced by exploiting this mechanism. Protein kinases related to DNA repair machinery are activated by being phosphorylated. Therefore we attempted making the RNA engineering technology, and will develop new radiation sensitization medicine as a molecular target drug for cancer. In this experiment, we are making RNA aptamers against phosphorylated site of DNA-PKcs by SELEX. In this research, we produced RNA aptamers specific to DNA-PKcs phosphorylation site. Furtheremore their specific template sequences were analyzed by next-generation sequencing technology, and some that may recognize DNA-PKcs specifically were focused. U2OS cells are transfected with the RNA molecules, we tested whether can increase sensitivity of radiation. In conclusion, we found some RNA molecules that enhance sensitivity of radiation for U2OS cells.

  • Research Products

    (14 results)

All 2011 2010 2009 Other

All Journal Article (5 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] NK314 potentiates anti'tumor activity with adult T-cell leukemia-lymphoma cellsby inhibition of dual targets on topoisomerase II{alpha}c and DNA-dependent protein kinase.2011

    • Author(s)
      Hisatomi T, Sueoka-Aragane N, Sato A, Tomimasu R, Ide M, Kurimasa A, Okamoto K, Kimura S, Sueoka E
    • Journal Title

      Blood 117(13)

      Pages: 3575-84

  • [Journal Article] Differential role of DNA-PKcs phosphorylations and kinase activity in radiosensitivity and chromosomal instability.2011

    • Author(s)
      Nagasawa H, Little JB, Lin YF, So S, Kurimasa A, Peng Y, Brogan JR, Chen DJ, Bedford JS, Chen BP
    • Journal Title

      Radiat Res 175

      Pages: 83-9

  • [Journal Article] Involvement of N-acetyltransferase human in the cytotoxic activity of 5-fluorouracil.2009

    • Author(s)
      Takubo K, Tsuchiya H, Kurimasa A, Arnesen T, Ryoke K, Shiota G.
    • Journal Title

      Anticaneer Drugs 20(8)

      Pages: 668-75

  • [Journal Article] SIRT2 down- regulation confers resistance to microtubule inhibitors by prolonging chronic mitotic arrest.2009

    • Author(s)
      Inoue T, Nakayama Y, Yamada H, Li YC, Yamaguchi S, Osaki M, Kurimasa A, Hiratsuka M, Katoh M, Oshimura M.
    • Journal Title

      Cell Cyele 8(8)

      Pages: 1279-91

  • [Journal Article] Effectiveness of combined treatment using X-rays and a phosphoinositide 3-kinase inhibitor, ZSTK474, on proliferation of HeLa cells in vitro and in vivo.

    • Author(s)
      Anzai K, Sekine-Suzuki E, Ueno M, Okamura M, Yoshimi H, Dan S, Yaguchi SI, Enami J, Yamori T, Okayasu R
    • Journal Title

      Caneer Sei. in press

  • [Presentation] Live Cell Imaging and kinetics of DNA double-strand breaks (DBSs)foci in cycling U2OS cells.2011

    • Author(s)
      Kurimasa A, Okuda S, Okada A, Tomimatsu N, Iwabuchi K, Chen DJ.
    • Organizer
      Genomic Instability and DNA repair in Keystone symposia on Molecular and Cellular Biology
    • Place of Presentation
      Keystone, Colorado, USA
    • Year and Date
      20110130-20110204
  • [Presentation] DNA resection by Exol dictates critical DNA repair and damage signaling decisions in response to DNA double-strand breaks.2011

    • Author(s)
      Tomimatsu N, Mukherjee B, Deland K, Kurimasa A, Porteus M, Bolderson E, Khanna KK, Burma S.
    • Organizer
      Genomic Instability and DNA repair in Keystone symposia on Molecular and Cellular Biology
    • Place of Presentation
      Keystone, Colorado, USA
    • Year and Date
      20110130-20110204
  • [Presentation] DNA二本鎖切断再結合におけるATMと53BP1の役割2010

    • Author(s)
      井原誠、小林純也、栗政明弘、小松賢志、工藤崇
    • Organizer
      日本放射線影響学会、第53回大会
    • Place of Presentation
      京都テルサ、京都
    • Year and Date
      20101020-20101022
  • [Presentation] Analysis of novel phosphorylation site of 53BPI induced by DNA double-strand breaks.2010

    • Author(s)
      Kurimasa A, Nagayoshi Y, Okuda S, Okada A, Tomimatsu N, Iwabuchi K, Chen DJ.
    • Organizer
      A-T International Workshop 2010
    • Place of Presentation
      Redondo Beach, California, USA
    • Year and Date
      20100411-20100414
  • [Presentation] Laser and ionizing radiation induced DSB fbci fbrmation : in vivo real time imaging using modified 53BP1-GFP fusion protein.2010

    • Author(s)
      Kurimasa A, Nagayoshi Y, Okuda S, Okada A, Tomimatsu N, Iwabuchi K, Chen DJ.
    • Organizer
      Recent Advances in DNA Repair and Related Studies 6th NIRS International Open Laboratory Workshop
    • Place of Presentation
      放射線医学総合研究所、千葉
    • Year and Date
      2010-03-24
  • [Presentation] DNA-PKリン酸化部位に対するRNAアプタマーの作製2009

    • Author(s)
      奥田沙奈絵、永吉悠里、岡田茜、押村光雄、汐田剛史、栗政明弘
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      20091001-20091003
  • [Presentation] DNA2本鎖切断により誘導される53BP1新規リン酸化部位の解析2009

    • Author(s)
      永吉悠里、奥田沙奈絵、岡田茜、押村光雄、汐田剛史、栗政明弘
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      20091001-20091003
  • [Presentation] がん放射線治療の展開と生物影響の基礎研究2009

    • Author(s)
      丹羽太貫
    • Organizer
      茨城大学理学部量子サイエンスフォーラム 公開シンポジウム第2回Quantum Medicine研究会
    • Place of Presentation
      茨城大学理学部(水戸市文京2-1-1)
    • Year and Date
      2009-01-31
  • [Remarks] ホームページ等

URL: 

Published: 2012-02-13   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi