2010 Fiscal Year Final Research Report
Development of the complete translation system reconstituted with human factors toward the synthesis of artificial viruses
| Project/Area Number |
20200056
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biofunction/Bioprocess
Virology
|
|---|
| Research Institution | University of Hyogo |
|---|
Principal Investigator |
IMATAKA Hiroaki University of Hyogo, 大学院・工学研究科, 教授 (50201942)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Keywords | 再構成 / ウイルス / タンパク質合成 / 翻訳 / セルフリーシステム |
|---|
| Research Abstract |
We succeeded in synthesizing encephalomyocarditis virus in the human cell ext-derived in vitro protein synthesis system and purifying the virus. We developed the translation system reconstituted with human translation factors using the hepatitis C virus RNA, translation elongation factors, releasing factors, ribosomes and aminoacylated tRNAs. By using this system, we attempted to synthesize the capsid proteins of encephalomyocarditis virus.
|
