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2010 Fiscal Year Final Research Report

Characterization and applications of enzymatic cross-linking reactions

Research Project

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Project/Area Number 20200072
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

Allocation TypeSingle-year Grants
Research Field Applied biochemistry
Applied molecular and cellular biology
Research InstitutionNagoya University

Principal Investigator

KIYOTAKA Hitomi  名古屋大学, 生命農学研究科, 准教授 (00202276)

Co-Investigator(Renkei-kenkyūsha) KAMIYA Noriho  九州大学, 大学院・工学系研究科, 教授 (50302766)
MTSUFUJI Senya  東京慈恵会医科大学, 医学部, 教授 (50192753)
Project Period (FY) 2008 – 2010
Keywords酵素 / 基質 / カルシウム
Research Abstract

Enzymatic cross-linking is essential reaction for several biological events: blood coagulation, skin formation. Transglutaminase, which is responsible for the reaction, is a family of enzyme consisting of 8 isozymes. In this project, I have carried out the identification of highly reactive substrate peptide which is useful tool for resolving of physiological significance. Furthermore, application of the peptide has been attempted.

  • Research Products

    (32 results)

All 2012 2011 2010 2009 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (21 results)

  • [Journal Article] Knocking-in the R142C mutation in transglutaminase 1 disrupts the stratum comeum barrier and postnatal survival of mice2012

    • Author(s)
      Nakagawa N, Yamamoto M., Hitomi K, (他 10名)Yamanishi K.
    • Journal Title

      J. Dermatol. Sci.

      Volume: 65 Pages: 192-206

    • Peer Reviewed
  • [Journal Article] Hornerin is a component of the epidermal comified cell envelopes2011

    • Author(s)
      Henry J, Hitomi K., Simon M., (他8名)
    • Journal Title

      FASEB J.

      Volume: 25(5) Pages: 1567-1576

    • Peer Reviewed
  • [Journal Article] Screening of substrate peptide sequences for tissue-type transglutaminase (TGase 2) Using T7 Phage cDNA Library2011

    • Author(s)
      Sugimura, Y. Yamashita H. Hitomi K.
    • Journal Title

      Cytotechnology

      Volume: 63(2) Pages: 111-118

    • Peer Reviewed
  • [Journal Article] In situ detection of active transglutaminases for keratinocyte-type (TGase 1) and tissue-type (TGase 2) using fluorescence-labeled highly reactive substrate peptides2011

    • Author(s)
      Itoh M., Kawamoto T., Tatsukawa H., Kojima S., Yamanishi, K., Hitomi. K.
    • Journal Title

      J. Histochem. Cytochem.

      Volume: 59 Pages: 180-187

    • Peer Reviewed
  • [Journal Article] Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles2011

    • Author(s)
      Yamane, A., Fukui, M., Sugimura, Y., Itoh, M., Alea, M. P, Said, El Alaoui, Akiyama, M., Hitomi, K.
    • Journal Title

      FEBS J.

      Volume: 277 Pages: 3564-3574

    • Peer Reviewed
  • [Journal Article] The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea2010

    • Author(s)
      Zeeuwen PM., van Vlijmen-Willems I., Rodijk-Olthuisl, D., Hitomi K., Schalkwijk J., (他5名)
    • Journal Title

      FASEB J.

      Volume: 24 Pages: 3744-3755

    • Peer Reviewed
  • [Journal Article] Transglutaminase 1 Preferred Substrate Peptide K5 Is an Efficient Tool in Diagnosis of Lamellar Ichthyosis2010

    • Author(s)
      Akiyama M., Hitomi K. and Shimizu, H., (他4名)
    • Journal Title

      Am. J. Pathol.

      Volume: 129 Pages: 2306-2309

    • Peer Reviewed
  • [Journal Article] A Specific Colorimetric assay for measuring of transglutaminase 1 and factor XIII activities2009

    • Author(s)
      Hitomi, K., Kitamura, M., Alea, M. P., Ismail, C., Thomas, V., Alaoui, S El.
    • Journal Title

      Anal. Biochem.

      Volume: 394 Pages: 281-283

    • Peer Reviewed
  • [Journal Article] A biochemical pathway that controls skin barrier formation: expression of its key components in inflammatory skin diseases and in reconstructed skin2009

    • Author(s)
      Cheng, T., Hitomi K., Schalkwijk, J. Zeeuwen, P.L.J.M., (他3名)
    • Journal Title

      Br J. Dermatol.

      Volume: 161 Pages: 253-264

    • Peer Reviewed
  • [Journal Article] タンパク質架橋化酵素の高反応性基質配列の探索と活用2009

    • Author(s)
      人見清隆
    • Journal Title

      生化学

      Volume: vol81.No.8. Pages: 708-711

    • Peer Reviewed
  • [Journal Article] Development of an isozyme-specific colorimetric assay for tissue transglutaminase 2 cross-linking assay2009

    • Author(s)
      Alea, M. P., Kitamura, M., Martin, G., Thomas, V., Hitomi, K., Alaoui, S El.
    • Journal Title

      Anal. Biochem.

      Volume: 389 Pages: 150-156

    • Peer Reviewed
  • [Presentation] Characterization of medaka transg/Utamirlases, a protein cross-linking enzyme family2011

    • Author(s)
      斉藤麻衣、樋口幾、伊藤みほ、人見清隆
    • Organizer
      Medaka Strategic Research
    • Place of Presentation
      岡崎(愛知県)
    • Year and Date
      20111100
  • [Presentation] タンパク質架橋化酵素トランスグルタミナーゼの高反応性基質配列の探索と多面的活用2011

    • Author(s)
      人見清隆
    • Organizer
      酵素工学会
    • Place of Presentation
      東京(東京大学山上会館)
    • Year and Date
      20110900
  • [Presentation] Identification of the preferred substrate sequences for transglutaminase and utility for detection of activity in an isozyme-specific manner2011

    • Author(s)
      人見清隆
    • Organizer
      日本生化学会大会シンポジウム
    • Place of Presentation
      京都国際会議場
    • Year and Date
      20110900
  • [Presentation] タンパク質架橋化酵素の高反応性基質配列の探索と活用2011

    • Author(s)
      伊藤みほ、福井美奈、鞍本克真、清水由隆、人見清隆
    • Organizer
      日本動物細胞工学会
    • Place of Presentation
      東京(東京大学山上会館)
    • Year and Date
      20110700
  • [Presentation] 機能未知なタンパク質架橋化酵素(TG6)の高反応性基質配列の探索2010

    • Author(s)
      福井美奈、山根亜沙佳、伊藤みほ、人見清隆
    • Organizer
      生化学会大会分子生物学会年会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      20101200
  • [Presentation] 表皮特異的タンパク質架橋化酵素(TG3)の高反応性基質配列の解析2010

    • Author(s)
      山根亜沙佳、福井美奈、伊藤みほ、人見清隆
    • Organizer
      生化学会大会分子生物学会年会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      20101200
  • [Presentation] 分化段階の骨芽細胞株におけるタンパク質架橋化酵素トランスグルタミナーゼの基質解析2010

    • Author(s)
      渡邊一哉、人見清隆
    • Organizer
      生化学会大会分子生物学会年会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      20101200
  • [Presentation] タンパク質架橋化酵素の高反応性基質ペプチドを活用したマウス全組織内酵素活性の可視化2010

    • Author(s)
      伊藤みほ、川本忠文、人見清隆
    • Organizer
      農芸化学会中部支部例会
    • Place of Presentation
      名古屋(名古屋大学)
    • Year and Date
      20101000
  • [Presentation] Screeninig and identification of the preferred substrate sequences for tranglutaminase 3 using phage-display peptide library2010

    • Author(s)
      山根亜沙佳、福井美奈、伊藤みほ、人見清隆
    • Organizer
      ゴードン国際会議
    • Place of Presentation
      米国
    • Year and Date
      20100700
  • [Presentation] Detection of in situ activities of transglutaminases using highly reactive substrate peptides2010

    • Author(s)
      伊藤みほ、川本忠文、人見清隆
    • Organizer
      ゴードン国際会議
    • Place of Presentation
      米国
    • Year and Date
      20100700
  • [Presentation] Identification of highly reactive substrate peptides for transglutaminases from random peptide library and its application2010

    • Author(s)
      人見清隆
    • Organizer
      ゴードン国際会議
    • Place of Presentation
      米国
    • Year and Date
      20100700
  • [Presentation] タンパク質架橋化酵素トランスグルタミナーゼの高反応性基質配列の解明と活用2010

    • Author(s)
      人見清隆
    • Organizer
      天野エンザイム応用酵素シンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      20100600
  • [Presentation] Establishment of a novel assay system for transglutaminase using highly reactive substrate peptide2010

    • Author(s)
      人見清隆
    • Organizer
      国際ポリアミン学会
    • Place of Presentation
      静岡(御殿場)
    • Year and Date
      20100600
  • [Presentation] ヒト表皮型タンパク質架橋化酵素(TGase 3)の高反応性基質配列の探索と解析2010

    • Author(s)
      福井美奈、山根亜沙佳、伊藤みほ、人見清隆, 他
    • Organizer
      生化学会中部支部例会
    • Place of Presentation
      名古屋(名古屋大学)
    • Year and Date
      20100500
  • [Presentation] ヒト表皮型トランスグルタミナーゼに対する高反応性基質配列の探索と解析2010

    • Author(s)
      福井美奈、山根亜沙佳、柴田秀樹、牧正敏、人見清隆
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      東京・東京大学
    • Year and Date
      20100300
  • [Presentation] 高等動物トランスグルタミナーゼの高反応性基質配列の探索と活用2010

    • Author(s)
      人見清隆
    • Organizer
      日本農芸化学会大会シンポジウム
    • Place of Presentation
      東京・東京大学
    • Year and Date
      20100300
  • [Presentation] トランスグルタミナーゼの骨芽細胞株における発現と架橋化酵素活性の解析2009

    • Author(s)
      渡辺一哉、角田佳奈子、柴田秀樹、牧正敏、人見清隆
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      20091000
  • [Presentation] タンパク質架橋酵素の高反応性基質ペプチドの探索とその有効活用2009

    • Author(s)
      人見清隆
    • Organizer
      第82回日本生化学会大会シンポジウム
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      20091000
  • [Presentation] 生体内タンパク質接着反応における高反応性基質と多面的活用2009

    • Author(s)
      人見清隆
    • Organizer
      慈恵会医科大学分子生物セミナー
    • Place of Presentation
      東京・慈恵会医科大学
    • Year and Date
      20090900
  • [Presentation]2009

    • Author(s)
      Kiyotaka HITOMI
    • Organizer
      ゴードン国際会議「ポリアミン」
    • Place of Presentation
      アメリカニューハンプシャー州
    • Year and Date
      20090600
  • [Presentation] トランスグルタミナーゼに対する高反応性基質ペプチドを活用した活性検出法の確立

    • Author(s)
      伊藤みほ、柴田秀樹、牧正敏、人見清隆
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      東京・東京大学

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Published: 2013-07-31  

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