Characterization and applications of enzymatic cross-linking reactions

2010 Fiscal Year Final Research Report

• Project/Area Number: 20200072
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry; Applied molecular and cellular biology
• Research Institution: Nagoya University
• Principal Investigator: KIYOTAKA Hitomi 名古屋大学, 生命農学研究科, 准教授 (00202276)
• Co-Investigator(Renkei-kenkyūsha): KAMIYA Noriho 九州大学, 大学院・工学系研究科, 教授 (50302766) ; MTSUFUJI Senya 東京慈恵会医科大学, 医学部, 教授 (50192753)
• Project Period (FY): 2008 – 2010
• Keywords: 酵素 / 基質 / カルシウム

Research Abstract

Enzymatic cross-linking is essential reaction for several biological events: blood coagulation, skin formation. Transglutaminase, which is responsible for the reaction, is a family of enzyme consisting of 8 isozymes. In this project, I have carried out the identification of highly reactive substrate peptide which is useful tool for resolving of physiological significance. Furthermore, application of the peptide has been attempted.

Research Products

• [Journal Article] Knocking-in the R142C mutation in transglutaminase 1 disrupts the stratum comeum barrier and postnatal survival of mice (2012)
  • Author(s): Nakagawa N, Yamamoto M., Hitomi K, (他 10名)Yamanishi K.
  • Journal Title: J. Dermatol. Sci. Volume: 65 Pages: 192-206
  • Peer Reviewed
• [Journal Article] Hornerin is a component of the epidermal comified cell envelopes (2011)
  • Author(s): Henry J, Hitomi K., Simon M., (他8名)
  • Journal Title: FASEB J. Volume: 25(5) Pages: 1567-1576
  • Peer Reviewed
• [Journal Article] Screening of substrate peptide sequences for tissue-type transglutaminase (TGase 2) Using T7 Phage cDNA Library (2011)
  • Author(s): Sugimura, Y. Yamashita H. Hitomi K.
  • Journal Title: Cytotechnology Volume: 63(2) Pages: 111-118
  • Peer Reviewed
• [Journal Article] In situ detection of active transglutaminases for keratinocyte-type (TGase 1) and tissue-type (TGase 2) using fluorescence-labeled highly reactive substrate peptides (2011)
  • Author(s): Itoh M., Kawamoto T., Tatsukawa H., Kojima S., Yamanishi, K., Hitomi. K.
  • Journal Title: J. Histochem. Cytochem. Volume: 59 Pages: 180-187
  • Peer Reviewed
• [Journal Article] Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles (2011)
  • Author(s): Yamane, A., Fukui, M., Sugimura, Y., Itoh, M., Alea, M. P, Said, El Alaoui, Akiyama, M., Hitomi, K.
  • Journal Title: FEBS J. Volume: 277 Pages: 3564-3574
  • Peer Reviewed
• [Journal Article] The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea (2010)
  • Author(s): Zeeuwen PM., van Vlijmen-Willems I., Rodijk-Olthuisl, D., Hitomi K., Schalkwijk J., (他5名)
  • Journal Title: FASEB J. Volume: 24 Pages: 3744-3755
  • Peer Reviewed
• [Journal Article] Transglutaminase 1 Preferred Substrate Peptide K5 Is an Efficient Tool in Diagnosis of Lamellar Ichthyosis (2010)
  • Author(s): Akiyama M., Hitomi K. and Shimizu, H., (他4名)
  • Journal Title: Am. J. Pathol. Volume: 129 Pages: 2306-2309
  • Peer Reviewed
• [Journal Article] A Specific Colorimetric assay for measuring of transglutaminase 1 and factor XIII activities (2009)
  • Author(s): Hitomi, K., Kitamura, M., Alea, M. P., Ismail, C., Thomas, V., Alaoui, S El.
  • Journal Title: Anal. Biochem. Volume: 394 Pages: 281-283
  • Peer Reviewed
• [Journal Article] A biochemical pathway that controls skin barrier formation: expression of its key components in inflammatory skin diseases and in reconstructed skin (2009)
  • Author(s): Cheng, T., Hitomi K., Schalkwijk, J. Zeeuwen, P.L.J.M., (他3名)
  • Journal Title: Br J. Dermatol. Volume: 161 Pages: 253-264
  • Peer Reviewed
• [Journal Article] タンパク質架橋化酵素の高反応性基質配列の探索と活用 (2009)
  • Author(s): 人見清隆
  • Journal Title: 生化学 Volume: vol81.No.8. Pages: 708-711
  • Peer Reviewed
• [Journal Article] Development of an isozyme-specific colorimetric assay for tissue transglutaminase 2 cross-linking assay (2009)
  • Author(s): Alea, M. P., Kitamura, M., Martin, G., Thomas, V., Hitomi, K., Alaoui, S El.
  • Journal Title: Anal. Biochem. Volume: 389 Pages: 150-156
  • Peer Reviewed
• [Presentation] Characterization of medaka transg/Utamirlases, a protein cross-linking enzyme family (2011)
  • Author(s): 斉藤麻衣、樋口幾、伊藤みほ、人見清隆
  • Organizer: Medaka Strategic Research
  • Place of Presentation: 岡崎(愛知県)
  • Year and Date: 20111100
• [Presentation] タンパク質架橋化酵素トランスグルタミナーゼの高反応性基質配列の探索と多面的活用 (2011)
  • Author(s): 人見清隆
  • Organizer: 酵素工学会
  • Place of Presentation: 東京(東京大学山上会館)
  • Year and Date: 20110900
• [Presentation] Identification of the preferred substrate sequences for transglutaminase and utility for detection of activity in an isozyme-specific manner (2011)
  • Author(s): 人見清隆
  • Organizer: 日本生化学会大会シンポジウム
  • Place of Presentation: 京都国際会議場
  • Year and Date: 20110900
• [Presentation] タンパク質架橋化酵素の高反応性基質配列の探索と活用 (2011)
  • Author(s): 伊藤みほ、福井美奈、鞍本克真、清水由隆、人見清隆
  • Organizer: 日本動物細胞工学会
  • Place of Presentation: 東京(東京大学山上会館)
  • Year and Date: 20110700
• [Presentation] 機能未知なタンパク質架橋化酵素(TG6)の高反応性基質配列の探索 (2010)
  • Author(s): 福井美奈、山根亜沙佳、伊藤みほ、人見清隆
  • Organizer: 生化学会大会分子生物学会年会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 20101200
• [Presentation] 表皮特異的タンパク質架橋化酵素(TG3)の高反応性基質配列の解析 (2010)
  • Author(s): 山根亜沙佳、福井美奈、伊藤みほ、人見清隆
  • Organizer: 生化学会大会分子生物学会年会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 20101200
• [Presentation] 分化段階の骨芽細胞株におけるタンパク質架橋化酵素トランスグルタミナーゼの基質解析 (2010)
  • Author(s): 渡邊一哉、人見清隆
  • Organizer: 生化学会大会分子生物学会年会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 20101200
• [Presentation] タンパク質架橋化酵素の高反応性基質ペプチドを活用したマウス全組織内酵素活性の可視化 (2010)
  • Author(s): 伊藤みほ、川本忠文、人見清隆
  • Organizer: 農芸化学会中部支部例会
  • Place of Presentation: 名古屋(名古屋大学)
  • Year and Date: 20101000
• [Presentation] Screeninig and identification of the preferred substrate sequences for tranglutaminase 3 using phage-display peptide library (2010)
  • Author(s): 山根亜沙佳、福井美奈、伊藤みほ、人見清隆
  • Organizer: ゴードン国際会議
  • Place of Presentation: 米国
  • Year and Date: 20100700
• [Presentation] Detection of in situ activities of transglutaminases using highly reactive substrate peptides (2010)
  • Author(s): 伊藤みほ、川本忠文、人見清隆
  • Organizer: ゴードン国際会議
  • Place of Presentation: 米国
  • Year and Date: 20100700
• [Presentation] Identification of highly reactive substrate peptides for transglutaminases from random peptide library and its application (2010)
  • Author(s): 人見清隆
  • Organizer: ゴードン国際会議
  • Place of Presentation: 米国
  • Year and Date: 20100700
• [Presentation] タンパク質架橋化酵素トランスグルタミナーゼの高反応性基質配列の解明と活用 (2010)
  • Author(s): 人見清隆
  • Organizer: 天野エンザイム応用酵素シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 20100600
• [Presentation] Establishment of a novel assay system for transglutaminase using highly reactive substrate peptide (2010)
  • Author(s): 人見清隆
  • Organizer: 国際ポリアミン学会
  • Place of Presentation: 静岡(御殿場)
  • Year and Date: 20100600
• [Presentation] ヒト表皮型タンパク質架橋化酵素(TGase 3)の高反応性基質配列の探索と解析 (2010)
  • Author(s): 福井美奈、山根亜沙佳、伊藤みほ、人見清隆, 他
  • Organizer: 生化学会中部支部例会
  • Place of Presentation: 名古屋(名古屋大学)
  • Year and Date: 20100500
• [Presentation] ヒト表皮型トランスグルタミナーゼに対する高反応性基質配列の探索と解析 (2010)
  • Author(s): 福井美奈、山根亜沙佳、柴田秀樹、牧正敏、人見清隆
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 東京・東京大学
  • Year and Date: 20100300
• [Presentation] 高等動物トランスグルタミナーゼの高反応性基質配列の探索と活用 (2010)
  • Author(s): 人見清隆
  • Organizer: 日本農芸化学会大会シンポジウム
  • Place of Presentation: 東京・東京大学
  • Year and Date: 20100300
• [Presentation] トランスグルタミナーゼの骨芽細胞株における発現と架橋化酵素活性の解析 (2009)
  • Author(s): 渡辺一哉、角田佳奈子、柴田秀樹、牧正敏、人見清隆
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 20091000
• [Presentation] タンパク質架橋酵素の高反応性基質ペプチドの探索とその有効活用 (2009)
  • Author(s): 人見清隆
  • Organizer: 第82回日本生化学会大会シンポジウム
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 20091000
• [Presentation] 生体内タンパク質接着反応における高反応性基質と多面的活用 (2009)
  • Author(s): 人見清隆
  • Organizer: 慈恵会医科大学分子生物セミナー
  • Place of Presentation: 東京・慈恵会医科大学
  • Year and Date: 20090900
• [Presentation] (2009)
  • Author(s): Kiyotaka HITOMI
  • Organizer: ゴードン国際会議「ポリアミン」
  • Place of Presentation: アメリカニューハンプシャー州
  • Year and Date: 20090600
• [Presentation] トランスグルタミナーゼに対する高反応性基質ペプチドを活用した活性検出法の確立
  • Author(s): 伊藤みほ、柴田秀樹、牧正敏、人見清隆
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 東京・東京大学