2010 Fiscal Year Final Research Report
Development immunomodulative drug based on the recognition mechanism of viral B-DNA.
| Project/Area Number |
20200074
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
Environmental pharmacy
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| Research Institution | Yokohama City University |
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Principal Investigator |
TAKESHITA Fumihiko Yokohama City University, 医学研究科, 客員教授 (60333572)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 感染防御 / 分子 / 基礎医学 |
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| Research Abstract |
Previous our data have discovered the novel H2B function to recognize non-chromosomal double stranded DNA (dsDNA), and induce antiviral innate immune response. In current research, we constructed the fusion protein, named as N'-CARD, by fusion of IPS-1 fragment with H2B fragment, and found that N'-CARD induced type I interferons (Type I IFNs) production. In addition, nuclear DNA helicase II was identified as N'-CARD associated molecules, and was indispensable for N'-CARD-mediated innate immune response. Furthermore, N'-CARD fused to protein transduction domain (N'-CARD-PTD) is a candidate of novel adjuvant. Therefore, current research revealed H2B-sensing machinery of cytoplasmic dsDNA as well as novel adjuvant candidate for vaccine development.
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[Journal Article] Immunogenicity of whole-parasite vaccines against Plasmodium falciparum involves malarial hemozoin and host TLR9.2010
Author(s)
Coban, C., Y. Igari, M. Yagi, T.Reimer, S.Koyama, T.Aoshi, K.Ohata, T.Tsukui, F.Takeshita, K.Sakurai, T.Ikegami, A.Nakagawa, T.Horii, G.Nunez, K.J.Ishii, S.Akira.
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Journal Title
Cell Host Microbe 7
Pages: 50-61
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[Journal Article] Extrachromosomal histone H2B mediates innate antiviral immune responses induced by intracellular double-stranded DNA.2010
Author(s)
Kobiyama, K., F.Takeshita, N.Jounai, A.Sakaue-Sawano, A.Miyawaki, K.J.Ishii, T.Kawai, S.Sasaki, H.Hirano, N.Ishii, K.Okuda, K.Suzuki.
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Journal Title
J Virol 84
Pages: 822-832
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[Journal Article] A signaling polypeptide derived from an innate immune adaptor molecule can be harnessed as a new class of vaccine adjuvant.2009
Author(s)
Kobiyama, K., F.Takeshita, K.J.Ishii, S.Koyama, T.Aoshi, S.Akira, A.Sakaue-Sawano, A.Miyawaki, Y.Yamanaka, H.Hirano, K.Suzuki, K.Okuda.
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Journal Title
J Immunol 182
Pages: 1593-1601
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