2010 Fiscal Year Final Research Report
Production of the safer induced pluripotent stem cells and their application for regenerative medicine
| Project/Area Number |
20200076
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
General medical chemistry
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| Research Institution | National Institute of Biomedical Innovation |
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Principal Investigator |
川端 健二 National Institute of Biomedical Innovation (50356234)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 発生生物学 / 再生医学 |
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| Research Abstract |
Induced pluripotent stem (iPS) cells were produced by threetransduction systems, retroviral vectors, lentiviral vectors, and adenoviral vectors. The highest production efficiency was obtained when lentiviral vectors were used. This is because lentiviral vectors could show the highest transduction efficiency. When HoxB4 gene, PPARγ gene, and Runx2 gene were transduced with adenoviral vectors with the CA promoter, hematopoietic progenitor cells, adipocytes, and osteoblasts were efficiently induced, respectively, in comparison with conventional differentiation methods. These results demonstrate that adenoviral vectors could be a useful tool for efficient differentiation from iPS cells.
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[Journal Article] Efficient generation of hepatoblasts from human ES cells and iPS cells by transient overexpression of homeobox gene HEX.2011
Author(s)
Inamura M., Kawabata K., Takayama K., Tashiro K., Sakurai F., Katayama K., Toyoda M., Akutsu H., Miyagawa Y., Okita H., Kiyokawa N., Umezawa A., Hayakawa T., Furue MK., Mizuguchi H.
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Journal Title
Mol. Ther. 19
Pages: 400-407
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