Development of Technology to Manipulate the Biological Functions of Stem Cells for Cell Therapy of Next Generation

2012 Fiscal Year Final Research Report

• Project/Area Number: 20220010
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kyoto University
• Principal Investigator: TABATA Yasuhiko 京都大学, 再生医科学研究所, 教授 (50211371)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Masya 京都大学, 再生医科学研究所, 准教授 (10332735) ; DEZAWA Mari 東北大学, 医学(系)研究科(研究院), 教授 (50272323) ; UMEZAWA Akihiko 独立行政法人国立成育医療研究センター, 生殖・細胞医療研究部, 部長 (70213486)
• Project Period (FY): 2008 – 2012
• Keywords: 細胞 / 組織工学

Research Abstract

A cationized gelatin of gene transfection was prepared by chemical introducing spermine to gelatin. Gelatin sponge scaffolds mechanically reinforced by ceramic granules incorporation were combined with growth factors and a complex of ca tionized gelatin and plasmid DNA. Bone marrow derived mesenchymal stem cells (MSC) were cultured for gene transfection in the sponge prepared. Comparing with the conventional gene transfection, a higher efficiency of MSC genetically engineering was observed. Upone combining with a bioreactor of culture medium circulating system, the engineering efficiency was further improved. This culture technology is applicable for small interfering DNA, and demonstrated to be a promising engineering technology of cell bioactivity.

Research Products

• [Journal Article] Gelatin nanospheres incorporating siRNA for controlled intracellular release (2012)
  • Author(s): Ishikawa H, Nakamura Y, Jo J, Tabata Y.
  • Journal Title: intracellular release Volume: 33(35) Pages: 9097-104
  • DOI: doi:10.1016/j.biomaterials.2012.80.032
  • Peer Reviewed
• [Journal Article] A gene transfection for rat mesenchymal stromal cells in biodegradable gelatin scaffolds containing cationized polysaccharides. (2011)
  • Author(s): Kido Y, Jo J, Tabata Y.
  • Journal Title: Biomaterials Volume: 32(3) Pages: 919-25
  • DOI: doi:10.1016/j.biomaterials2010.09.056
  • Peer Reviewed
• [Journal Article] Combined transplantation of bone marrow stromal cell-derived neural progenitor cells with a collagen sponge and fibroblast growth factor (bFGF) releasing microspheres enhances recovery after cerebral ischemia in rats. (2011)
  • Author(s): Matsuse D, Kitada M, Ogura F , WakaoS, Kohama M, Kira J, Tabata Y, Dezawa M.
  • Journal Title: Tissue Eng Part A Volume: 17(15-16) Pages: 1993-2004
  • DOI: doi:10.1089/ten.TEA2010.0585
  • Peer Reviewed
• [Journal Article] Promoted adipogenesis of rat mesenchymal stem cells by transfection of small interfering RNA complexed with a cationized dextran. (2010)
  • Author(s): Nagane K, Jo J, Tabata Y.
  • Journal Title: Tissue Engineering Volume: 16(1) Pages: 21-31
  • DOI: doi:10.1089/ten.TEA2009.0170
  • Peer Reviewed
• [Journal Article] Preparation of cationized polysaccharides as gene transfection carrier for bone marrow-derived mesenchymal stem cells. (2010)
  • Author(s): Jo J, Okazaki A, Nagane K, Yamamoto M, Tabata Y.
  • Journal Title: J Biomater Sciences Polym Eds Volume: 21(2) Pages: 185-204
  • DOI: doi:10.1163/156856209X415495
  • Peer Reviewed
• [Journal Article] Practical induction system for dopamine-producing cells from bone marrow stromal cells by spermine-pullulan-mediated reverse transfection method. (2009)
  • Author(s): Nagane K, Kitada, M, Wakao S, Dezawa M, Tabata Y.
  • Journal Title: Tissue Eng Part A Volume: 15(7) Pages: 1655-65
  • DOI: doi:10.1089/ten.tea2008.0453
  • Peer Reviewed
• [Presentation] 細胞への遺伝子導入に与える培養基材の影響. (2012)
  • Author(s): 上田真澄、田畑泰彦
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20120612-14
• [Presentation] コアセルベーション法よる siRNA 含有ナノ粒子の作製 (2011)
  • Author(s): 石川英史、城潤一郎、田畑泰彦
  • Organizer: 第27回日本 DDS 学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20110609-10
• [Presentation] Biomaterial technology to manipulate stem cells for regenerative medicine and therapy. (2010)
  • Author(s): Tabata Y.
  • Organizer: TERMIS-AP 2010
  • Place of Presentation: Sydney Australia
  • Year and Date: 20100915-17
• [Presentation] Promoted adipogenic differentiation of rat mesenchymal stem cells by transfection of small interfering RNA complexed with a cationized dextran. (2010)
  • Author(s): Jo J, Nagane K, Kido Y , and Tabata Y.
  • Organizer: 37th Annual Meeting and Exposition of the Controlled Release Society
  • Place of Presentation: OregonUSA
  • Year and Date: 20100710-14
• [Presentation] 生体吸収性の三次元細胞足場を利用した組織幹細胞の遺伝子改変 (2009)
  • Author(s): 木戸祐一郎, 城 潤一郎, 田畑泰彦
  • Organizer: 第31回日本バイオマテリアル学会大会
  • Place of Presentation: 京都
  • Year and Date: 20091116-17
• [Presentation] 生体吸収性の三次元細胞足場内の幹細胞への新規遺伝子導入法の開発. (2009)
  • Author(s): 木戸祐一郎, 城 潤一郎, 田畑泰彦
  • Organizer: 第25回日本 DDS 学会
  • Place of Presentation: 東京
  • Year and Date: 20090703-04
• [Presentation] 生体シグナル因子を組み込んだバイオマテリアルによる 3次元組織構築. (2009)
  • Author(s): 山本雅哉, 田畑泰彦
  • Organizer: 第8回日本再生医療学会
  • Place of Presentation: 東京
  • Year and Date: 20090305-06
• [Presentation] Efficient induction system of neural precursor cells and dopamine-producing cells from bone marrow stromal cells. (2009)
  • Author(s): Dezawa M
  • Organizer: Academy for Multidisciplinary Neurotraumatology (AMN) The Hong Kong Neurosurgery Society (HKNS) Conjoint Congress
  • Place of Presentation: 新界(香港)
  • Year and Date: 2009-11-14
• [Book] 第2 編:再生医療に必要な"技術"とは 第4 章 細胞の遺伝子改変技術.ものづくり技術からみる再生医療 -細胞研究・創薬・治療- (2011)
  • Author(s): 城潤一郎、田畑泰彦
  • Total Pages: 125-132
• [Book] 遺 伝 子 医 学MOOK 別冊 ますます重要になる細胞周辺環境(細胞ニッチ)の最新科学技術 (2009)
  • Author(s): 城潤一郎、田畑泰彦
  • Total Pages: 369
  • Publisher: 株式会社メディカルドゥ
• [Book] 遺伝子導入法「進みつづける細胞移植治療の実際(上巻)遺伝子医学 MOOK 別冊 再生医療の実現に向けた科学・技術と周辺要素の理解」 (2008)
  • Author(s): 城潤一郎、田畑泰彦
  • Total Pages: 218-225
  • Publisher: 株式会社メディカルドゥ、大阪
• [Remarks] 幹細胞 骨→脂肪へ路線変更 . 産経新聞2010.1.31
• [Remarks] -よくわかる再生医療に必要なものづくり
• [Remarks] -再生治療と細胞研究を支えるモノづくりガイドブック. 2011.2月発行. 4000部
• [Remarks] -よくわかる再生医療に必要なものづくり
• [Remarks] -再生治療と細胞研究を支えるモノづくりガイドブック. 2010.6月発行. 4000部
• [Remarks] (1) 田畑泰彦:モノづくり技術から見た再生医療ビジネス -再生治療と再生研究の違い-.-専門家との直接意見交換シンポジウム in KRP PartV- (2012.9.26 京都)
• [Remarks] (2) 田畑泰彦:モノづくり技術が支える再生医療の実用化 -細胞研究、創薬研究、治療-、専門家との直接意見交換シンポジウム in KRP Part IV (2011.9.21 京都)
• [Remarks] (3) 田畑泰彦:モノづくり技術からみた再生医療.バイオの都・関西セミナー ～再生医療の未来を拓く関西～(2010.12.9大阪)
• [Remarks] (4) 田畑泰彦:再生医療の基本概念と再生医療分野の研究開発とモノづくり技術との関係. 再生医療を支えるモノづくりシンポジウム. (2010.8.31 京都) 300 人