Fathoming the evolution of gene regulation through an 'arms race' between tansposons and Argonautes

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20221008
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: 基礎ゲノム科学
• Research Institution: Keio University
• Principal Investigator: SIOMI Haruhiko Keio University, 医学部, 教授 (60202107)
• Project Period (FY): 2008 – 2012
• Keywords: 転移因子 / RNAサイレンシング / Argonaute / ゲノム / 進化

Research Abstract

ヒトやマウスのmiRNA-AGO2複合体に代表される小分子RNAとArgonauteタンパク質の複合体(RNA Induced Silencing Complexes/RISCs)が関与する遺伝子抑制機構はRNAサイレンシングと呼ばれる。転移因子(transposable element/TE)とRNAサイレンシング機構の間の'軍拡競争'の結果が複雑な遺伝子発現制禦を進化させ、それらが特にArgonauteを中核とする「生命活動を支えるプログラム」に組み込まれてきたことを理解する。

Research Products

• [Journal Article] Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. (2010)
  • Author(s): Saito, K., Ishizu, H., Komai, M., Kotani, H., Kawamura, Y., Nishida, KM., Siomi, H., Siomi, MC.
  • Journal Title: Genes, Development 24 Pages: 2493-2498
• [Journal Article] Biogenesis pathways of piRNAs loaded onto AGO3 in the Drosophila. (2010)
  • Author(s): Nagao, A., Mituyama, T., Huang, H., Chen, D., Siomi, MC., Siomi, H.
  • Journal Title: RNA 16 Pages: 2503-2515
• [Journal Article] A direct role of Hsp90 in pre-RISC formation in Drosophila (2010)
  • Author(s): Miyoshi, T., Takeuchi, A., Siomi, H., Siomi, M.C.
  • Journal Title: Nature Structural and Molecular Biology 17 Pages: 1024-1026
• [Journal Article] How does the royal family of Tudor rule the Piwi-interacting RNA pathway? (2010)
  • Author(s): Siomi, M.C., Mannen, T., Siomi, H.
  • Journal Title: Genes & Development 24 Pages: 636-646
• [Journal Article] Functional involvement of Tudor and dPRMT5 in the piRNA processing pathway in Drosophila germlines. (2009)
  • Author(s): Nishida, KM., Okada, TN., Kawamura, T., Mituyama, T., Kawamura, Y., Inagaki, S., Huang, H., Chen, D., Kodama, T., Siomi, H., Siomi, MC.
  • Journal Title: EMBO J. 28 Pages: 3820-3831
• [Journal Article] A regulatory circuit for piwi by traffic jam, a large Maf, in Drosophila gonadal somas (2009)
  • Author(s): Saito, K., Inagaki, S., Mituyama, T., Kawamura, Y., Ono, Y., Sakota, E., Kotani, H., Asai, K., Siomi H., Siomi, MC.
  • Journal Title: Nature 461 Pages: 1296-1299
• [Journal Article] RISC hitches onto endosome trafficking. (2009)
  • Author(s): Siomi, H., Siomi, MC.
  • Journal Title: Nature Cell Biology 11 Pages: 1049-1051
• [Journal Article] On the road to reading the RNA interference code (2009)
  • Author(s): Siomi, H., Siomi, MC.
  • Journal Title: Nature 457 Pages: 396-404
• [Journal Article] Drosophila endogenous small RNAs bind to Argonaute2 in somatic cells. (2008)
  • Author(s): Kawamura, Y., Saito, K., Kin, T., Ono, Y., Asai, K., Sunohara, T., Okada, NT., Siomi MC., Siomi, H.
  • Journal Title: Nature 453 Pages: 793-797
• [Presentation] International Symposium on Control of Gene Expression and Cancer (2010)
  • Organizer: dedicated to the 20th anniversary of the Institute of Gene Biology, Russian Academy of Sciences
  • Place of Presentation: Moscow, Russia
  • Year and Date: 20100621-20100625
• [Presentation] Plenary Lecture, RNA Symposium 2010 (2010)
  • Organizer: (the 3rd RNA molecular Biology meeting of Taiwan), National Cheng Kung University
  • Place of Presentation: Tainan, Taiwan
  • Year and Date: 20100208-20100209
• [Presentation] Piwi-interacting RNAs (piRNAs), the guardians of the germ-line stem cell genome ?Biogenesis and Function- (2010)
  • Organizer: The New York Academy of Sciences
  • Place of Presentation: New York
  • Year and Date: 2010-11-03
• [Presentation] "Bingzhi Forum" speaker at Institute of Zoology (2010)
  • Organizer: Chinese Academy of Sciences
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-06-15
• [Presentation] Genetics Seminar Program (2010)
  • Organizer: Yale University School of Medicine
  • Place of Presentation: New Haven
  • Year and Date: 2010-01-12
• [Presentation] RiboClub 2008 Opening Session Program ‘Tiny RNAs-big players in the RNA world', (2008)
  • Organizer: Sherbrooke, Quebec,
  • Place of Presentation: Canada
  • Year and Date: 20080922-20080924
• [Book] RNA Technologies and Their Applications(eds., V.Erdmann and J.Barciszewski)(The key features of RNA silencing.) (2010)
  • Author(s): Saito, K., Miyoshi, K., Siomi, MC., Siomi, H.
  • Total Pages: 1-28
  • Publisher: Springer-Verlag Berlin Heidelberg