Functional Analysis of Newly-found Actin Structure Involved in Chloroplast Photorelocation Movement

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20227001
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyushu University
• Principal Investigator: WADA Masamitsu Kyushu University, 大学院・理学研究院, 特任教授 (60011681)
• Project Period (FY): 2008 – 2012
• Keywords: 葉緑体 / アクチン繊維 / 光応答 / フォトトロピン / シロイヌナズナ / シダ

Research Abstract

葉緑体光定位運動は、植物が効率良く光合成を行なうためのみならず、強光下での光障害を避けるために必須の生理現象である。従来、細胞内に既存のアクチン繊維を介した運動と考えられていたが、我々は、今迄に報告の無い新規の葉緑体光定位運動に働く葉緑体アクチン(cp-actin)繊維を発見した。葉緑体独自の細胞小器官移動様式としてのcp-actin繊維の構造と機能の解明を通して葉緑体運動のメカニズムを明らかにする。

Research Products

• [Journal Article] Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis. (2010)
  • Author(s): Kodama, Y., N. Suetsugu, S.-G. Kong, M. Wada
  • Journal Title: Proc.Natl.Acad.Sci.USA 107 Pages: 19591-19596
  • Peer Reviewed
• [Journal Article] Two kinesin-like proteins mediate actin-based chloroplast movement in Arabidopsis thaliana. (2010)
  • Author(s): Suetsugu, N,. N. Yamada, T. Kagawa, H. Yonekura, T.Q.P. Uyeda, A. Kadota, M. Wada
  • Journal Title: Proc.Natl.Acad.Sci.USA 107 Pages: 8860-8865
  • Peer Reviewed
• [Journal Article] Crystallographic and functional analyses of J-domain of JAC1 essential for chloroplast photo relocation movement in Arabidopsis thaliana (2010)
  • Author(s): Takano, A., N. Suetsugu, M. Wada, D. Kohda
  • Journal Title: Plant Cell Physiol. 51 Pages: 1372-1376
  • Peer Reviewed
• [Journal Article] Short actin-based mechanism for light-directed chloroplast movement in Arabidopsis. (2009)
  • Author(s): Kadota, A., N. Yamada, N. Suetsugu, M. Hirose, C. Saito, K. Shoji, S. Ichikawa, T. Kagawa, A. Nakano, M. Wada
  • Journal Title: Proc.Natl.Acad.Sci.USA 106 Pages: 13106-13111
  • Peer Reviewed
• [Journal Article] Chloroplast outer envelope protein CHUP1 is essential for chloroplast anchorage to the plasma membrane and chloroplast movement. (2008)
  • Author(s): Oikawa, K., A. Yamasato, S.-G. Kong, M. Kasahara, M. Nakai, F. Takahashi, Y. Ogura, T. Kagawa, M. Wada
  • Journal Title: Plant Physiol. 148 Pages: 829-842
  • Peer Reviewed
• [Presentation] Factors controlling the existence of chloroplast actin filaments involved in chloroplast movement. (2010)
  • Author(s): Wada, M.
  • Organizer: Gordon Research Conference on "Photosensory Receptors & Signal Transduction".
  • Place of Presentation: Il Ciocco, Italy(Invited speaker)
  • Year and Date: 20100418-20100423
• [Presentation] The mechanism of chloroplast photorelocation movement. (2009)
  • Author(s): Wada, M.
  • Organizer: 9th IPMB Congress
  • Place of Presentation: St.Louis, Missouri, USA(Invited speaker)
  • Year and Date: 20091025-20091030
• [Presentation] Chloroplasts move towards or away from light, why and how? (2009)
  • Author(s): Wada, M.
  • Organizer: 15th International Congress on Photobiology
  • Place of Presentation: Dusseldorf, Germany(Presidential lecture as the President)
  • Year and Date: 20090718-20090723
• [Presentation] The mechanisms of chloroplast photorelocation movement. (2009)
  • Author(s): Wada, M.
  • Organizer: Keystone Symposium `Plant Sensing, Response and Adaptation to the Environment', Org.by S.Kay & J.Chory, Big Sky
  • Place of Presentation: Montana, USA(Invited speaker)
  • Year and Date: 20090111-20090116
• [Presentation] Photoperception and the mechanism of chloroplast photorelocation movement. (2008)
  • Author(s): Wada, M.
  • Organizer: The Ins and Outs of Chloroplasts
  • Place of Presentation: Osaka University, Osaka(Plenary lecture)
  • Year and Date: 2008-10-14
• [Book] Plant Cell Monographs, The Chloroplast-Interaction with Environment(Chloroplast photorelocation movement)(Ed.AS Sandelius and H Aronsson) (2009)
  • Author(s): Suetsugu, N., M. Wada
  • Total Pages: 235-266
  • Publisher: Springer
• [Book] Arabidopsis Methods in Molecular Biology (Analysis of chloroplast movement and relocation)(Ed.RP Jarvis)
  • Author(s): Wada, M., S-G. Kong
  • Publisher: Humana Press in press
• [Remarks] 西日本新聞学校教育欄「理科大好き=葉緑体」2010年1月12日
• [Remarks] 岐阜新聞サイエンスズームアップ欄「強い光から逃げる葉緑体」2010年1月7日
• [Remarks] Science 326 : 1058 MEETINGBRIEFS欄「Chloroplast shuffle」2009年11月20日
• [Remarks] 朝日新聞科学欄「葉緑体に新たな運動機構」2009年8月11日
• [Remarks] ホームページ
  • URL: http://wadalab.biology.kyushu-u.ac.jp/