2011 Fiscal Year Final Research Report
Cell Turgor Measurement - Probe Electrospray Ionization (PESI) Mass Spectrometry for Cell Molecular Profiling Techniques
| Project/Area Number |
20228004
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Agricultural environmental engineering
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| Research Institution | Ehime University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIRAOKA Kenzo 山梨大学, クリーンエネルギー研究センター, 特命教授 (80107218)
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| Research Collaborator |
ERRA-BALSELLS Rosa ブエノスアイレス大学, 基礎自然学部(大学間学術交流協定・学部間学術交流協定締結校), 教授
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| Project Period (FY) |
2008-05-12 – 2013-03-31
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| Keywords | 生物環境調節 / 植物 / 細胞・組織 / 探針エレクトロスプレー / 質量分析 / スピーキング・セル・アブローヂ / 膨圧計測 / ブレヮシャーブローブ |
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| Research Abstract |
A cell pressure probe measures cell turgor of plants, and can extract cell solution directly from actively growing plants. The pressure probe technique and the probe electrospray ionization (PESI) mass spectrometry (MS) can be combined together in order to analyze cell molecular components in intact growing crops. When a small amount of engine oil additives was mixed with silicon oil in the pressure probe, ionization of cell metabolites was successfully conducted for MS analyses. By using physiological molecular information, environmental conditionscan be adjusted optimally to grow crops in plant growth factories.
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[Journal Article] Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry.2009
Author(s)
Yu, Z., Chen, L.C., Suzuki, H., Ariyada, O., Erra-Balsells, R., Nonami, H., Hiraoka, K
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Journal Title
Journal of the American Society for Mass Spectrometry
Volume: 20 (12)
Pages: 2304-2311
DOI
Peer Reviewed
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