Study of Shigella infectious strategy for the intestinal barrier

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20229006
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: The University of Tokyo
• Principal Investigator: SASAKAWA Chihiro The University of Tokyo, 医科学研究所, 教授 (70114494)
• Project Period (FY): 2008 – 2011
• Keywords: 病原性 / 赤痢菌 / III型分泌機構 / エフェクター / 自然免疫 / 細胞死 / ユビキチン

Research Abstract

本研究では、T3SSから分泌される一群のエフェクターに着目し、赤痢菌の上皮固有バリアーと自然免疫バリアーの克服に関わるエフェクターとその標的因子を同定し、それら細胞内機能を、分子、細胞、組織、個体の各レベルで精査する。感染の各過程におけるこれらエフェクターと宿主標的因子との相互作用を時空列的に解析するとともに、他の粘膜病原細菌の類似なエフェクターとも比較解析し、これら知見を統合して、粘膜病原細菌の新たな感染現象と腸粘膜バリアに対する普遍的および特異的な感染戦略を明らかにする。

Research Products

• [Journal Article] Bacterial interactions with the host epithelium. (2010)
  • Author(s): Kim M, Ashida H, Ogawa M, Yoshikawa Y, Mimuro H, Sasakawa C.
  • Journal Title: Cell Host Microbe. 8 Pages: 20-35
• [Journal Article] A bacterial E3 ubiquitin ligase IpaH9.8 targets NEMO/IKKgamma to dampen the host NF-kappaB-mediated inflammatory response. (2010)
  • Author(s): Ashida H, Kim M, Schmidt-Supprian M, Ma A, Ogawa M, Sasakawa C.
  • Journal Title: Nat Cell Biol. 12 Pages: 66-73
• [Journal Article] Listeria monocytogenes ActA-mediated escape from autophagic recognition. (2009)
  • Author(s): Yoshikawa Y, Ogawa M, Hain T, Yoshida M, Fukumatsu M, Kim M, Mimuro H, Nakagawa I, Yanagawa T, Ishii T, Kakizuka A, Sztul E, Chakraborty T, Sasakawa C.
  • Journal Title: Nat Cell Biol. 11 Pages: 1233-1240
• [Journal Article] Bacteria hijack integrin-linked kinase to stabilize focal adhesions and block cell detachment. (2009)
  • Author(s): Kim M, Ogawa M, Fujita Y, Yoshikawa Y, Nagai T, Koyama T, Nagai S, Lange A, Fassler R, Sasakawa C.
  • Journal Title: Nature 459 Pages: 578-582
• [Journal Article] Helicobacter pylori CagA phosphorylation-independent function in epithelial proliferation and inflammation. (2009)
  • Author(s): Suzuki M, Mimuro H, Kiga K, Fukumatsu M, Ishijima N, Morikawa H, Nagai S, Koyasu S, Gilman RH, Kersulyte D, Berg DE, Sasakawa C.
  • Journal Title: Cell Host Microbe. 5 Pages: 23-34
• [Presentation] New insights into Shigella-gut epithelium interaction (2010)
  • Author(s): Sasakawa C.
  • Organizer: AHMR Forum 2010-Health and medical research in the Asia Pacific region : the opportunities, the possibilities, the challenges.
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2010-11-18
• [Presentation] New insights into Shigella-gut epithelium interaction (2010)
  • Author(s): Sasakawa C.
  • Organizer: Embo Workshop, Emerging Themes in infection Biology
  • Place of Presentation: Nice, France
  • Year and Date: 2010-06-02
• [Presentation] Autophagy Evasion by Cytosolic Bacteria (2009)
  • Author(s): Sasakawa C.
  • Organizer: 109^<th> General Meeting of the American Society for Microbiology
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2009-05-18
• [Presentation] Shigella infection of intestinal mucosa and host response (2008)
  • Author(s): Sasakawa C.
  • Organizer: The 9^<th> Korea-Japan International Symposium on Microbiology
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2008-10-17
• [Presentation] Shigella modulerar immunsvaret (2008)
  • Author(s): Sasakawa C.
  • Organizer: Swedish Microbiologist Meeting
  • Place of Presentation: Umea, Sweden
  • Year and Date: 2008-06-04
• [Remarks] Nature 2009関連記事:Nature DIGEST(2009, 6:3)「細菌が乗っ取る腸上皮細胞」
  • URL: http://www.ims.u-tokyo.ac.jp/bac/hp/mainpage.html
• [Remarks] 日経BP社アカデミック(2009,8月)「東大医科研の笹川教授ら、病原細菌が腸管感染を拡大する巧妙な戦術を発見」
• [Remarks] Newton(2009,9月7日)「赤痢菌のハイジャック」