2010 Fiscal Year Final Research Report
Integrative Study of transcriptional network systems during enchondral ossification
| Project/Area Number |
20229010
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
YONEDA Toshiyuki Osaka University, 大学院・歯学研究科, 教授 (80142313)
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| Co-Investigator(Renkei-kenkyūsha) |
NISHIMURA Riko 大阪大学, 大学院・歯学研究科, 准教授 (60294112)
HATA Kenji 大阪大学, 大学院・歯学研究科, 講師 (80444496)
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| Project Period (FY) |
2008 – 2010
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| Keywords | Sox9 / 軟骨分化 / 転写制御 / Runx2 |
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| Research Abstract |
Enchondral ossification is very unique and complex biological event which is harmoniously and strictly regulated by several growth factors and cytokines. These factors regulate enchondral ossification by controlling intracellular signaling and transcription factors. Transcription factors, Sox9 and Runx2, play essential roles in enchondral ossification. We found that p54^<nrb> couples transcription to mRNA maturation during enchondral ossification by forming transcriptional complex with Sox9. In contrast, Arid5a and Znf219, both of which form transcriptional complex with Sox9, play a role in chondrocyte differentiation in association with Sox9. In addition, we found that Sox9 forms negative-feedback loop with Ihh/Gli signaling for late stage of chondrogenesis by up-regulating PTHrP expression. Furthermore, we identified that a transcription factor, Dmrt2, links Sox9 function to Runx2. Collectively, our findings provide novel insights into molecular basis of enchondral ossification.
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[Journal Article] Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription.2011
Author(s)
Amano K, Hata K, Muramatsu S, Wakabayashi M, Takigawa Y, Ono K, Nakanishi M, Takashima R, Kogo M, Matsuda A, Nishimura R, Yoneda T
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Journal Title
Mol Biol Cell 22
Pages: 1300-1311
Peer Reviewed
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[Journal Article] Regulation of ER stress response by BBF2H7/Sec23a pathway is essential for chondrogenesis.2009
Author(s)
Saito A, Hino S, Murakami T, Kondo S, Kanemoto S, Sekiya H, Saitoh M, Nishimura R, Yoneda T, Furuichi T, Ikegawa S,Ikawa M, Okabe M, ImaizumiK
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Journal Title
Nature Cell Biol 11
Pages: 1197-1204
Peer Reviewed
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[Journal Article] The signaling mediated by the ER stress transducer. OASIS is involved in bone formation.2009
Author(s)
Murakami T, Saito A, Hino S, Kondo S, Kanemoto S, Chihara K, Sekiya H, Tsumagari K, Ochiai K, Yoshinaga K,Saitoh M, Nishimura R, Yoneda T, Kou I, Furuichi T, Ikegawa I, Ikawa M, Okabe M, Wanaka A, Imaizumi K
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Journal Title
Nature Cell Biol 11
Pages: 1205-1211
Peer Reviewed
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[Journal Article] PIAS3 negatively regulates RANKL-mediated osteoclastogenesis directly in osteoclast precursors and indirectly via osteoblasts.2008
Author(s)
Hikata T, Takaishi H, Takito J, Hakozaki A, Furukawa M, Uchikawa S,Kimura T, Okada Y, Matsumoto M, Yoshimura A, Nishimura R, Reddy SV, Asahara H, Toyama Y
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Journal Title
Blood 113
Pages: 2202-2212
Peer Reviewed
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