Molecular Mechanisms of Structural Plasticity of Dendritic Spine

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20240032
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: HAYASHI Yasunori The Institute of Physical and Chemical Research, シナプス機能研究チーム, チームリーダー (90466037)
• Project Period (FY): 2008 – 2012
• Keywords: シナプス可塑性 / 樹状突起スパイン / CaMKII / 細胞骨格 / 海馬

Research Abstract

近年我々は海馬長期増強(LTP)ならびに長期抑圧(LTD)の誘導に伴い樹上突起spineの形態が急速に変化する事を見いだし、シナプスの構造がこれまで考えられてきたよりダイナミックな事を示した。しかしNMDA受容体の活性化からこの「構造可塑性」に至る分子機構は明らかではない。本申請は、シナプス後部に多量に存在するシグナル分子であるCa^<2+>/calmodulin-dependent protein kinase(CaMKII)に注目し、CaMKIIがF-actinを束化する事で細胞骨格の一員として機能する事を示し、そのシナプスの機能的、構造的可塑性との関係について理解する事を主目的とする

Research Products

• [Journal Article] The postsynaptic density proteins Homer and Shank form a polymeric network structure. (2009)
  • Author(s): Hayashi, M.K., Tang, C., Verpelli, C., Narayanan, R., Stearns, M.H., Xu, R.-M., Li, H., Sala, C., Hayashi, Y.
  • Journal Title: Cell 137 Pages: 159-171
  • URL: http://glutamate.brain.riken.jp/dokuwiki/hayashi_cell.pdf
  • Peer Reviewed
• [Journal Article] Functional dynamics of polo-like kinase-1 at the centrosome. (2009)
  • Author(s): Kishi, K., van Vugt, M.A., Okamoto, K.-I., Hayashi, Y., Yaffe, M.B.
  • Journal Title: Mol Cell Biol. 29 Pages: 3134-3150
  • URL: http://glutamate.brain.riken.jp/dokuwiki/kishi_mol_cell_biol.pdf
  • Peer Reviewed
• [Presentation] Sequential reorganization of synaptic components after LTP induction. (2010)
  • Author(s): Hayashi, Y.
  • Organizer: Gordon Research Conferences Synaptic Transmission
  • Place of Presentation: Biddeford, ME, USA.
  • Year and Date: 20100725-20100730
• [Presentation] Regulation of structural function of CaMKII by multiple autophosphorylation sites. (2010)
  • Author(s): Hayashi, Y., Karam K., Hayashi M., Matsuura K., Narayanan R., Lakhanpal G., Okamoto K.
  • Organizer: 7th FENS Forum of European Neuroscience
  • Place of Presentation: Amsterdam, Nederland.
  • Year and Date: 20100703-20100707
• [Book] Introduction of green fluorescent protein (GFP) into hippocampal neurons through viral infection. (2010)
  • Author(s): Malinow R., Hayashi, Y., Maletic-Savatic M., Zaman S.H., Poncer J.-C., Shi S.-H., Esteban J.A., Osten P., Seidenman K.
  • Total Pages: 5406
  • Publisher: In Cold Spring Harbor Protoc.
• [Book] Neuronal activity-induced genes and synaptic plasticity, tagging and capture. In Synaptic plasticity in health and disease (ed. Kreutzer and Sala, Springer Verlag, in the press)
  • Author(s): Hayashi, Y., Okamoto, K., Bosch, M., Futai K.
• [Remarks] ウェッブページ
  • URL: http://glutamate.brain.riken.jp
• [Remarks] Public relation
• [Remarks] 「世界脳週間 夏休み高校生理科教室」研究室見学 2010.8.20
• [Remarks] 税務大学校 理化学研究所見学会に伴う講演会 2011.1.17