Molecular mechanisms of structural plasticity of dendritic spine

2012 Fiscal Year Final Research Report

• Project/Area Number: 20240032
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: HAYASHI Yasunori 独立行政法人理化学研究所, 記憶メカニズム研究チーム, チームリーダー (90466037)
• Project Period (FY): 2008 – 2012
• Keywords: 細胞・組織 / シグナル伝達 / 神経科学 / 生理学 / 脳・神経 / 記憶学習 / シナプス可塑性

Research Abstract

We found autophosphorylation of CaMKII on multiple serine and threonine residues on F-actin binding domain detaches CaMKII from F-actin. We concluded that CaMKII bundles F-actin under basal neuronal activity but when it is activated, it releases F-actin, thereby allowing its modification. This results in structural plasticity. In this way, we speculate that CaMKII act as a gate of structural plasticity.

Research Products

• [Journal Article] A rapid and effective FISH protocol with hybridization-sensitive fluorescent linear oligo probes (2012)
  • Author(s): Wang, D.O., Matsuno, H., Ikeda, S., Nakamura, A., Yanagisawa, H., Hayashi, Y., Okamoto, A
  • Journal Title: RNA Volume: 18 Pages: 166-175
• [Journal Article] Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo (2011)
  • Author(s): Mower, A. F., Kwok, S. M., Yu, H., Majewska, A. K., Okamoto, K.-I., Hayashi, Y.*, Sur, M.* (*: co-corresponding authors)
  • Journal Title: Proc. Natl. Acad. Sci Volume: 108 Pages: 21241-21246
• [Journal Article] The postsynaptic density proteins Homer and Shank form a polymeric network structure (2009)
  • Author(s): Hayashi, M.K., Tang, C., Verpelli, C., Narayanan, R., Stearns, M.H., Xu, R.-M., Li, H., Sala, C., Hayashi, Y
  • Journal Title: Cell Volume: 137 Pages: 159-171
• [Presentation] CaMKII is a gating mechanism of activity-induced structural modification of hippocampal dendritic spines (2013)
  • Author(s): Hayashi Y
  • Organizer: Winter Conference on Brain Science, Breckenridge
  • Place of Presentation: Colorado, USA Jan
  • Year and Date: 2013-05-02
• [Presentation] A virtual navigation system for two-photon calcium imaging in mice (2012)
  • Author(s): Masaaki Sato, Tanvir Islam, Takashi Takekawa, Hiroshi Yamakawa, Masako Kawano, Yoko Yamaguchi, Tomoki Fukai, Masamichi Ohkura, Junichi Nakai, Yasunori Hayashi
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120900
• [Presentation] Molecular mechanism of hippocampal learning and memory (2012)
  • Author(s): Hayashi Y
  • Organizer: 3rd LIN Symposium, Translating synaptic activity into neuronal plasticity
  • Place of Presentation: Tangermunde, Germany
  • Year and Date: 20120800
• [Presentation] CaMKII serves as a gate of actin-mediated structural plasticity of dendritic spine (2012)
  • Author(s): Karam Kim, Gurpreet Lakhanpal, Akio Suzuki, Mariko Hayashi, Radhakrishnan Narayanan, Tomoki Matsuda, Takeharu Nagai, Kenichi Okamoto, Yasunori Hayashi
  • Organizer: Gordon Research Conference Synaptic Transmission
  • Place of Presentation: Waterville Valley, USA
  • Year and Date: 20120700
• [Presentation] Molecular mechanism of hippocampal learning and memory (2012)
  • Author(s): Hayashi Y
  • Organizer: The 7th International Conference for Neurons and Brain Disease (Association for the Study of Neurons and Diseases)
  • Place of Presentation: Montreal, Canada
  • Year and Date: 20120600
• [Presentation] Molecular mechanism of hippocampal learning and memory (2012)
  • Author(s): Hayashi Y
  • Organizer: 2012 International Brain Research Symposium
  • Place of Presentation: Daegu, Korea
  • Year and Date: 20120500
• [Presentation] A quantitative anyalysis of AMPA receptor GluR1 phospho-isotypes (2011)
  • Author(s): Hosokawa, T., Hayashi, Y
  • Organizer: SfN 2011
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20111100
• [Presentation] The role of CaMKII beta in modulating dendritic spine structural plasticity (2011)
  • Author(s): Lakhanpal, G., Kim, K., Kato-Hayashi, M., Hayashi, Y., Okamoto, K
  • Organizer: SfN 2011
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20111100
• [Presentation] CaMKII gates rapid structural plasticity in hippocampal dendritic spines (2011)
  • Author(s): Kim, K., Hayashi, M., Narayanan, R., Suzuki, A., Matsuura, K., Okamoto, K., Hayashi, Y
  • Organizer: SfN 2011
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20111100
• [Presentation] Principle and application of fluorescence microscopy in neuroscience (2011)
  • Author(s): Hayashi, Y
  • Organizer: IBRO School
  • Place of Presentation: Beijing, China
  • Year and Date: 20111000
• [Presentation] A quantitative analysis of AMPA receptor GluR1 phospho-isotypes (2011)
  • Author(s): Hosokawa, T., Hayashi, Y
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110900
• [Presentation] In vivo two-photon imaging of neuronal circuit activity using transgenic mice that express fluorescent calcium sensor proteins in the brain (2011)
  • Author(s): Sato, M., Kawano, M., Islam, T., Yamaguchi, Y., Ohkura, M., Nakai, J., Hayashi, Y
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110900
• [Presentation] Structural roles of CaMKII (2011)
  • Author(s): Hayashi Y
  • Organizer: 第54回日本神経化学会
  • Place of Presentation: 金沢
  • Year and Date: 20110900
• [Presentation] The regulation of dendritic spine structural plasticity by CaMKII beta (2011)
  • Author(s): Hayashi Y
  • Organizer: 8th IBRO World Congress of Neuroscience
  • Place of Presentation: Florence, Italy
  • Year and Date: 20110700
• [Presentation] Phosphoisotype analysis of AMPA receptor GluR1 (2011)
  • Author(s): Hayashi Y
  • Organizer: Gordon Research Conference Excitatory Synapses & Brain Function
  • Place of Presentation: Easton, MA, USA
  • Year and Date: 20110600
• [Presentation] Temporal progression of the structural and molecular remodeling of dendritic spines after LTP induction (2011)
  • Author(s): Hayashi Y
  • Organizer: Neuronal Communication Beyond the Synaptic Cleft - International conference and workshop
  • Place of Presentation: Niigata
  • Year and Date: 20110200
• [Presentation] Sequential reorganization of synaptic components after LTP induction (2010)
  • Author(s): Hayashi Y
  • Organizer: Gordon Research Conferences Synaptic Transmission
  • Place of Presentation: Biddeford, ME, USA
  • Year and Date: 20100700
• [Presentation] Regulation of structural function of CaMKII by multiple autophosphorylation sites (2010)
  • Author(s): Hayashi Y
  • Organizer: 7^(th) FENS Forum of European Neuroscience
  • Place of Presentation: Amsterdam
  • Year and Date: 20100700
• [Presentation] Molecular mechanism of hippocampal synaptic plasticity (2010)
  • Author(s): Hayashi Y
  • Organizer: Bristol 2010 From molecules to neuronal
  • Place of Presentation: Bristol, UK
  • Year and Date: 20100600
• [Book] Catching the engram: strategies to examine the memory trace (2012)
  • Author(s): Sakaguchi M, Hayashi Y
  • Total Pages: 5:32
  • Publisher: Mol Brain
• [Book] The Ca2+ and Rho GTPase signaling pathways underlying activity-dependent actin remodeling at dendritic spines (2012)
  • Author(s): Saneyoshi T, Hayashi Y
  • Total Pages: 69(8):545-54
  • Publisher: Cytoskeleton (Hoboken)
• [Book] Roles of neuronal activity-induced gene products in Hebbian and homeostatic synaptic plasticity, tagging, and capture (2012)
  • Author(s): Hayashi Y, Okamoto K, Bosch M, Futai K
  • Total Pages: 970:335-54
  • Publisher: Adv Exp Med Biol
• [Book] Structural plasticity of dendritic spines (2012)
  • Author(s): Bosch M, Hayashi Y
  • Total Pages: 22(3):383-8
  • Publisher: Curr Opin Neurobiol
• [Remarks]
  • URL: http://glutamate.brain.riken.jp