2010 Fiscal Year Final Research Report
Development of in vivo sonoporation method using short-pulsed ultrasound with microbubbles adjacent to cells.
| Project/Area Number |
20240053
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical systems
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| Research Institution | Hokkaido University |
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Principal Investigator |
KUDO Nobuki Hokkaido University, 大学院・情報科学研究科, 准教授 (30271638)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Ryo 帝京大学, 薬学部, 講師 (90384784)
YAMAMOTO Katsuyuki 北海道大学, 大学院・情報科学研究科, 教授 (10088867)
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| Research Collaborator |
OKADA Kengo 北海道大学, 大学院・情報科学研究科, 博士課程学生
SKAGUCHI Katsuji 北海道大学
YAGI Tomofumi 北海道大学
WATANABE Noriko 北海道大学
CHIDA Yuuki 北海道大学
OKUYAMA Manabu 北海道大学
MATSUI Tomoko 北海道大学
YOSHIMATU Yuri 北海道大学, 大学院・情報科学研究科, 修士課程学生
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| Project Period (FY) |
2008 – 2010
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| Keywords | ソノポレーション / 超音波治療 / 遺伝子導入 / ドラッグデリバリ / 微小気泡 / タイムラプス観察 / 光ピンセット / ターゲティング気泡 |
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| Research Abstract |
The final goal of this study was to develop a safe and efficient method for in vivo sonoporation that uses short-pulsed ultrasound in the presence of microbubbles adjacent to cells. A light microscopy system was developed for time-lapse observation of changes in cells generated during sonoporation and used to study methods to improve efficiency of the sonoporation. Optical tweezers were included in the system to control the position and extent of membrane damage by changing bubble size and position. Cell membrane damage and repair, which occur in a period shorter than 20 minutes, were observed using the system and their mechanisms were investigated. The observation chamber was improved to enable observation of long-term cell changes initiated by sonoporation, and the period of time-lapse observation was extended up to 20 hours. Two types of functional bubbles, tissue-targeting bubbles and drug-loaded bubbles, were developed, and their effectiveness for improving the efficacy of the proposed sonoporation method was confirmed.
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[Journal Article] Cancer gene therapy by IL-12 gene delivery using liposomal bubbles and tumoral ultrasound exposure2010
Author(s)
Suzuki R, Namai E, Oda Y, Nishiie N, Otake S, Koshima R, Hirata K, Taira Y, Utoguchi N, Negishi Y, Nakagawa S, Maruyama K
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Journal Title
J Control Release 142(2)
Pages: 245-50
Peer Reviewed
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