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2012 Fiscal Year Final Research Report

Identification and automated counting of egg and larvae of zooplankton by molecular probes and its application to life history analysis

Research Project

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Project/Area Number 20241003
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental dynamic analysis
Research InstitutionThe University of Tokyo

Principal Investigator

TSUDA Atsushi  東京大学, 大気海洋研究所, 教授 (80217314)

Co-Investigator(Kenkyū-buntansha) MACHIDA Ryuji  東京大学, 大気海洋研究所, 特任研究員 (40401294)
Project Period (FY) 2008 – 2012
Keywords分子マーカー / 動物プランクトン / 自動計測 / 生物海洋 / 環境計測
Research Abstract

The coal of this study was to developments of species identification with molecular probes and automated counting system. We have developed species identification methods by real-time PCR for Neocalanus copepods, although we have failed to develop a automated counting system with flow-cytometer by technical problems. Using this method, we revealed early life histories of three species of Neocalanus copepods. Especially, one species of Neocalanus newly showed dormancy during their nauplius stages.

  • Research Products

    (36 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (14 results) (of which Peer Reviewed: 13 results) Presentation (20 results) Book (2 results)

  • [Journal Article] Species identification of calanoid copepods in the subtropical regions off the coast of Japan by using a new molecular marker ITS2-28S D1/D2.2013

    • Author(s)
      Hirai, J., S. Shimode and A. Tsuda
    • Journal Title

      Journal of Plankton Research

    • DOI

      doi:10.1093/plankt/fbt016

    • Peer Reviewed
  • [Journal Article] Processes and patterns of oceanic nutrient limitation.2013

    • Author(s)
      Moore, C.M., M. M. Mills, K. R. Arrigo,I. Berman-Frank, L. Bopp, P. W. Boyd, E. D. Galbraith, R. J. Geider, C. Guieu, S. L. Jaccard, T. D. Jickells, J. La Roche, T. M. Lenton, N. M. Mahowald, E. Maranon, I. Marinov, J. K. Moore, T. Nakatsuka, A. Oschlies, M. A. Saito, T. F. Thingstad, A. Tsuda and O. Ulloa
    • Journal Title

      Nature Geoscience

    • DOI

      doi:10.1038/NGEO1765

    • Peer Reviewed
  • [Journal Article] Distribution and life history of two planktonic copepods, Rhincalanus nasutus and Rhincalanus rostrifrons, in the northwestern Pacific Ocean.2012

    • Author(s)
      Shimode, S, K. Takahashi, Y. Shimizu, T. Nonomura and A. Tsuda
    • Journal Title

      Deep Sea Research I

      Volume: 65 Pages: 133-145

    • Peer Reviewed
  • [Journal Article] Life history of Eucalanus californicus Johnson (Copepoda: Clanoida) in the northwestern PacificOcean.2012

    • Author(s)
      Shimode, S., K. Takahashi, A. Tsuda
    • Journal Title

      Progress in Oceanography

      Volume: 96 Pages: 1-13

    • Peer Reviewed
  • [Journal Article] 夏季オホーツク西部海域における動物プランクトンの分布-クリオネの起源を追って2012

    • Author(s)
      津田敦、西岡純、伊東宏
    • Journal Title

      海洋と生物

      Volume: 34 Pages: 25-34

  • [Journal Article] Major phylogenetic groups of marine bacteria differ in growth response to natural phytoplankton blooms in the western North Pacific.2011

    • Author(s)
      Tada, Y., A. Taniguchi, I. Nagao, T.Miki, M. Uematsu, A. Tsuda, and K. Hamasaki
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 77 Pages: 4055-4065

    • Peer Reviewed
  • [Journal Article] Morphological characters for practical identification of the copepodite stages of three sympatric Calanus species in the western North Pacific.2011

    • Author(s)
      Nonomura, T, S. Nishida, A. Tsuda, I.Yasuda
    • Journal Title

      Journal of Plankton Research

      Volume: 33 Pages: 1496-1509

    • Peer Reviewed
  • [Journal Article] Response of lower trophic organisms to nutrients input and effects on carbon budget: a mesocosm experiment.2010

    • Author(s)
      Tsuda, A., K. Fukami, H. Kiyosawa, K.Suzuki, S. Takeda, J. Nishioka, M. Takahashi, K.W. Johnson, C. S. Wong
    • Journal Title

      Plankton and Benthos Research

      Volume: 5 Pages: 144-155

    • Peer Reviewed
  • [Journal Article] Dissimilarity of species and forms of the planktonic Neocalanus copepods using mitocondorial COI, 12S, nuclear ITS, and 28S gene sequence.2010

    • Author(s)
      Machida, J.R. and A. Tsuda
    • Journal Title

      PLOS One

      Volume: 5(4) Pages: 10278

    • DOI

      doi:10.1371/journal.pone.0010278.

    • Peer Reviewed
  • [Journal Article] Complete mitochondrial genome sequences of the three pelagic chaetognaths Sagitta nagae, Sagitta decipiens and Sagitta enflata.2010

    • Author(s)
      Miyamoto H, Machida RJ, Nishida S
    • Journal Title

      Comparative Biochemistry and Physiology D-Genomics and Proteomics

      Volume: 5 Pages: 65-72

    • Peer Reviewed
  • [Journal Article] Zooplankton diversity analysis through single-gene sequencing of a community sample2009

    • Author(s)
      Machida RJ, Hashiguchi Y, Nishida M, Nishida S
    • Journal Title

      BMC Genomics

      Volume: 10 Pages: 438

    • Peer Reviewed
  • [Journal Article] Life history and ontogenetic vertical migration of Neocalanus gracilis (Dana) in the western North Pacific.2009

    • Author(s)
      Shimode, S., Y. Hiroe, K. Hidaka, K.Takahashi and A. Tsuda
    • Journal Title

      Aquatic Biology

      Volume: 7 Pages: 295-306

    • Peer Reviewed
  • [Journal Article] Meso- and microzooplankton responses to an in situ iron fertilization experiment (SEEDS-II) in the northwest subarctic Pacific.2009

    • Author(s)
      Tsuda, A., H. Saito, R.J. Machida, S.Shimode
    • Journal Title

      Deep-Sea Research II

      Volume: 56 Pages: 2767-2778

    • Peer Reviewed
  • [Journal Article] 亜寒帯北太平洋における動物プランクトン津田敦を中心とした低次生態系の動態に関する研究

    • Author(s)
      津田敦
    • Journal Title

      海の研究

    • Peer Reviewed
  • [Presentation] 亜熱帯循環におけるカイアシ類のメタ28S rDNA解析2013

    • Author(s)
      平井惇也
    • Organizer
      日本海洋学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2013-03-23
  • [Presentation] A metagenomic method for marine planktonic copepods using 454 pyrosequncing of 28S rDNA gene2013

    • Author(s)
      Hirai, J
    • Organizer
      ASLO meeting
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2013-02-18
  • [Presentation] Early life cycle of Neocalanus copepods in the Oyashio region, western North Pacific2013

    • Author(s)
      Fujioka, H.
    • Place of Presentation
      New Orleans, USA.
    • Year and Date
      2013-02-18
  • [Presentation] Early life cycle of Neocalanus plumchrus and Neocalanus flemingeri in the Oyashio region, western north Pacific2012

    • Author(s)
      Fujioka, H
    • Organizer
      PICES Annual Meeting
    • Place of Presentation
      広島
    • Year and Date
      2012-10-24
  • [Presentation] Ontogenetic vertical migration of two tropical-subtropical copepods, Rhincalanus nasutus and Rhincalanus rostrifrons, in the northwestern Pacific Ocean:Implication for a variety of life history strategies of Rhincalanus. Pacific2012

    • Author(s)
      Shimode, S
    • Organizer
      PICES Annual Meeting
    • Place of Presentation
      広島
    • Year and Date
      2012-10-23
  • [Presentation] 黒潮周辺海域におけるカイアシ類のメタゲノム解析2012

    • Author(s)
      平井惇也
    • Organizer
      日本海洋学会秋季大会
    • Place of Presentation
      清水
    • Year and Date
      2012-09-14
  • [Presentation] 亜寒帯北太平洋における動物プランクトンを中心とした低次生態系の動態に関する研究2012

    • Author(s)
      津田敦
    • Organizer
      日本海洋学会春季大会
    • Place of Presentation
      つくば、(招待講演)
    • Year and Date
      2012-03-28
  • [Presentation] カイアシ類Neocalanus plumchrusの親潮域における初期生活史2012

    • Author(s)
      藤岡秀文
    • Organizer
      日本海洋学会春季大会
    • Place of Presentation
      つくば
    • Year and Date
      2012-03-26
  • [Presentation] 亜熱帯太平洋におけるEucalanidae科カイアシ類の成長に伴う鉛直移動と生活史戦略2011

    • Author(s)
      下出信次
    • Organizer
      東京大学大気海洋研究所共同利用研究集会、亜熱帯太平洋のプランクトン生態系および物質循環に関する比較海洋学
    • Place of Presentation
      東京
    • Year and Date
      2011-11-25
  • [Presentation] ITS2-28S rDNA配列による亜熱帯性カラヌス目カイアシ類の種同定と展望2011

    • Author(s)
      平井惇也
    • Organizer
      東京大学大気海洋研究所共同利用研究集会、亜熱帯太平洋のプランクトン生態系および物質循環に関する比較海洋学
    • Place of Presentation
      東京
    • Year and Date
      2011-11-25
  • [Presentation] Neocalanus and Calanus copepods in the western North Pacific,their distribution and life histories2011

    • Author(s)
      Tsuda, A
    • Organizer
      6th ADOES Workshop
    • Place of Presentation
      Qindao、China
    • Year and Date
      2011-11-18
  • [Presentation] 浮遊性カイアシ類Neocalanus属とCalanus属の分布特性と生活史2011

    • Author(s)
      下出信次、
    • Organizer
      2011年度日本ベントス学 会・日本 プランクトン学会合同大会
    • Place of Presentation
      高知
    • Year and Date
      2011-09-03
  • [Presentation] DNAシークエンスによる亜熱帯性Calanoidaカイアシ類の種同定技術の開発と展2011

    • Author(s)
      平井惇也
    • Organizer
      2011年度日本ベントス学会・日本プランクトン学会合同大会
    • Place of Presentation
      高知
    • Year and Date
      2011-09-03
  • [Presentation] Comparative study of the life histories of Eucalanidae copepods in the subtropical and subarctic Pacific2011

    • Author(s)
      Tsuda. A.
    • Organizer
      ESSAS Open Science Meeting
    • Place of Presentation
      Seattle, USA
    • Year and Date
      2011-05-21
  • [Presentation] Neocalanus vs. Calanusoceans. Comparative study on the life histories of Neocalanus and Calanuscopepods, and their global distribution2011

    • Author(s)
      Tsuda. A.
    • Organizer
      ESSAS Open Science Meeting
    • Place of Presentation
      Seattle, USA
    • Year and Date
      2011-05-21
  • [Presentation] West meets East:Inter-gyre transportation of Neocalanus copepods2010

    • Author(s)
      Saito, H.
    • Organizer
      PICES 19th Annual Meeting
    • Place of Presentation
      Portland USA
    • Year and Date
      2010-10-20
  • [Presentation] Identification of Neocalanus plumchrus and N.flemingeri2010

    • Author(s)
      Fujioka, H.
    • Organizer
      PICES 19th Annual Meeting
    • Place of Presentation
      Portland USA
    • Year and Date
      2010-10-20
  • [Presentation] Real-Time PCRを用いたNeocalanus plumchrusおよびN.flemingeri同定法の確立2010

    • Author(s)
      藤岡秀文
    • Organizer
      日本海洋学会2010年度春季大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-26
  • [Presentation] Vertical distribution of large suspension feeding copepods in the Oyashio region during the growing period2009

    • Author(s)
      Tsuda, A.
    • Organizer
      PICES 18th Annual Meeting
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      2009-10-23
  • [Presentation] Vertical and horizontal carbon transport through the ontogenetic vertical migration of copepods2009

    • Author(s)
      Saito, H.
    • Organizer
      Chapman conference on the Biological Carbon Pump of the Oceans
    • Place of Presentation
      Hampshire,England.
    • Year and Date
      2009-09-17
  • [Book] 生物学辞典2010

    • Author(s)
      津田敦
    • Total Pages
      1615
    • Publisher
      東京化学同人、東京
  • [Book] 水圏生物科学入門2009

    • Author(s)
      津田敦
    • Total Pages
      37-48
    • Publisher
      恒星社厚生閣

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Published: 2014-08-29  

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