2010 Fiscal Year Final Research Report
Analysis of DNA damage response using gene-disrupted DT40 clones.
| Project/Area Number |
20241012
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Kyoto University |
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Principal Investigator |
TAKEDA Shunichi Kyoto University, 大学院・医学研究科, 教授 (60188191)
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| Co-Investigator(Kenkyū-buntansha) |
MOTEGI Akira 京都大学, 大学院・医学研究科, 助教 (80452332)
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| Project Period (FY) |
2008 – 2010
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| Keywords | DNA組換え / 標的組換え / 遺伝子治療 / DT40 / ニワトリBリンパ細胞 |
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| Research Abstract |
Homologous recombination (HR) plays a major role in repairing DNA damage caused by chemotherapeutic agents, such as camptothecin and cisplatin. HR is carried out by coordinate actions of more than 50 different proteins. We have analyzed the role of the individual HR proteins by disrupting genes encoding the HR proteins in the chicken DT40 cells. We have revealed the function of the following HR proteins, including CtIP, DNA polymerases θ and ν, Fan1, Palb-2, Sfr1, Slx4, and Sws1.
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[Journal Article] Collaborative action of Brca1 and CtIP in elimination of covalent modifications from double-strand breaks to facilitate subsequent break repair.2010
Author(s)
Nakamura K, Kogame T, Oshiumi H, Shinohara A, Sumitomo Y, Agama K, Pommier Y, Tsutsui KM, Tsutsui K, Hartsuiker E, Ogi T, Takeda S, Taniguchi Y.
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Journal Title
PLoS Genet. 6
Pages: e1000828
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[Journal Article] Simultaneous disruption of two DNA polymerases, Polη and Polζ, in avian DT40 cells unmasks the role of Polη in cellular response to various DNA lesions.2010
Author(s)
Hirota K, Sonoda E, Kawamoto T, Motegi A, Masutani C, Hanaoka F, Szuts D, Iwai S, Sale JE, Lehmann A, Takeda S.
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Journal Title
PLoS Genet. 6
Pages: e1001151
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[Journal Article] GEMIN2 promotes accumulation of RAD51 at double-strand breaks in homologous recombination.2010
Author(s)
Takizawa Y, Qing Y, Takaku M, Ishida T, Morozumi Y, Tsujita T, Kogame T, Hirota K, Takahashi M, Shibata T, Kurumizaka H, Takeda S.
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Journal Title
Nucleic Acids Res. 38
Pages: 5059-74
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[Journal Article] Geneticevidence for single-strand lesions initiating Nbs1-dependent homologous recombination in diversification of IgV in chicken B lymphocytes.2009
Author(s)
Nakahara M, Sonoda E, Nojima K, Sale JE, Takenaka K, Kikuchi K, Taniguchi Y, Nakamura K, Sumitomo Y, Bree RT, Lowndes NF, Takeda S.
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Journal Title
PLoS Genet. 5
Pages: e1000356
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[Journal Article] DNA damage response protein ASCIZ links base excision repair with immunoglobulin gene conversion.2008
Author(s)
Oka H, Sakai W, Sonoda E, Nakamura J, Asagoshi K, Wilson SH, Kobayashi M, Yamamoto K, Heierhorst J, Takeda S, Taniguchi Y.
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Journal Title
Biochem Biophys Res Commun. 371
Pages: 225-9
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