2011 Fiscal Year Final Research Report
Development of single genome DNA methylation detection device for epigenetics analysis
| Project/Area Number |
20241032
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TOKESHI Manabu 北海道大学, 工学研究院, 教授 (60311437)
KAJI Noritada 名古屋大学, 大学院・工学研究科, 准教授 (90402479)
OKAMOTO Yukihiro 名古屋大学, 革新ナノバイオデバイス研究センター, 特任講師 (50503918)
ZHANG Yong 名古屋大学, 工学研究科, 研究員 (40467329)
WANG Jun 名古屋大学, 工学研究科, 研究員 (30543532)
YASUI Takao 名古屋大学, 工学研究科, 助教 (00630584)
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| Project Period (FY) |
2008 – 2011
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| Keywords | エピジェネティクス / マイクロ・ナノデバイス / DNA伸長 / 1分子計測 |
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| Research Abstract |
We successfully developed novel DNA methylation analysis method. Our method elongates single DNA by flow or nanospace in the microfluidic device and detect methylation site with methylation point specific quantum-dots. Therefore, we need no troublesome procedures like PCR and can identify the location of methylation site as well as can detect methylation in single molecule level, which cannot attain by current methylation analysis method. These advantages would permit our method to be applied for medical diagnosis.
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[Journal Article] Size-selective growth and stabilization of small CdSe nanoparticles in aqueous solution2010
Author(s)
Park, Yeon-Su ; Dmytruk, Andriy ; Dmitruk, Igor ; Kasuya, Atsuo ; Takeda, Motohiro ; Ohuchi, Noriaki ; Okamoto, Yukihiro ; Kaji, Noritada ; Tokeshi, Manabu ; Baba, Yoshinobu
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Journal Title
ACS Nano
Volume: 4
Pages: 121-128
DOI
Peer Reviewed
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[Presentation] Microfluidic sensor for the detection of DNA or protein by hybridization-based fluorescence enhancement or immunoassay-based fluorescence quenching2010
Author(s)
Jun Wang, Michihiko Aki, Daisuke Onoshima, Kenji Arinaga, Noritada Kaji, Manabu Tokeshi, Shozo Fujita, Naoki Yokoyama, Yoshinobu Baba
Organizer
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS2010)
Place of Presentation
Martiniplaza, Groningen, Netherlands
Year and Date
20101003-07
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