2011 Fiscal Year Final Research Report
A modular strategy to construct functional RNA-protein complexes
| Project/Area Number |
20241051
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Kyoto University |
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Principal Investigator |
MORII Takashi 京都大学, エネルギー理工学研究所, 教授 (90222348)
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| Co-Investigator(Kenkyū-buntansha) |
TAINAKA Kazuki 京都大学, エネルギー理工学研究所, 助教 (80506113)
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| Project Period (FY) |
2008 – 2011
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| Keywords | RNA / ペプチド / インビトロセレクション / リセプター / センサー / 分子認識 |
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| Research Abstract |
A stable complex of a peptide and RNA, ribonucleopeptide(RNP), provides a new framework to construct a macromolecular receptor for small molecules. The RRE RNA and the Rev peptide form a structurally well-characterized stable RNP complex that is suitable for a stepwise functionalization. In vitro selection of the RNP pool originating from an RRE-based RNA library and the Rev peptide affords RNP receptors specific for nucleotide triphosphates, for a phosphotyrosine residue in defined amino acid sequence, and for dopamine. A new ATP-binding motif for the ATP-binding RNP was characterized by a combination of biochemical and NMR studies. The RNP receptor functionalized by a fluorophore-labeled Rev peptide exerts optical signals associated with the ligand binding events. Replacing the Rev peptide of the ATP-binding RNP with a fluorophore-modified Rev peptide affords a series of fluorescent ATP sensors. This strategy to generate tailor-made fluorescent sensors is applied for a selective detection of a specific phosphorylated tyrosine residue within a defined amino acid sequence.
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