Community analysis of subsurface biosphere responsible for methane cycling

2010 Fiscal Year Final Research Report

• Project/Area Number: 20244089
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Geochemistry/Astrochemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: KAMAGATA Yoichi National Institute of Advanced Industrial Science and Technology, 生物プロセス研究部門, 研究部門長 (70356814)
• Co-Investigator(Kenkyū-buntansha): TAMAKI Hideyuki 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究員 (00421842) ; SAKATA Susumu 独立行政法人産業技術総合研究所, 地圏資源環境研究部門, 研究グループ長 (70357101) ; YOSHIOKA Hideyoshi 独立行政法人産業技術総合研究所, 地圏資源環境研究部門, 研究員 (30415765) ; TAKEUCHI Mio 独立行政法人産業技術総合研究所, 地圏資源環境研究部門, 研究員 (20357403) ; SUZUKI Yohei 独立行政法人産業技術総合研究所, 地圏資源環境研究部門, 研究員 (00359168)
• Project Period (FY): 2008 – 2010
• Keywords: 天然ガス / 油田 / 地下圏生物 / メタン菌 / メタン酸化菌 / 地球化学

Research Abstract

To shed light on the subsurface methane cycling microbial communities, we have analyzed the community structures, cultivated the dominant organisms, and analyzed the basic traits of isolated organisms : (1) we were successful in isolating dominant hydrogenotrophic methanogens in an association water sample in a natural gas field. The isolate was found to be distinct from know methanogens ; (2) acetate was found to be the most important intermediate in a deep subsurface oil reservoir in which syntrophic acetate oxidation occurred ; (3) anaerobic methane oxidation was detected in terrestrial subsurface samples ; (4) a syntrophic aerobic methane oxidizing community that comprised at least three members of bacteria was constructed.

Research Products

• [Journal Article] A distinct freshwater-adapted subgroup of ANME-1 dominates active archaeal communities in terrestrial subsurfaces in Japan. (2011)
  • Author(s): Takeuchi, M., H.Yoshioka, Y.Seo, S.Tanabe, H.Tamaki, H.A.Takahashi, S.Igari, D.Mayumi, S.Sakata
  • Journal Title: Environmental Microbiology in press
  • Peer Reviewed
• [Journal Article] A six-well plate method: less laborious and effective method for cultivation of obligate anaerobic microorganisms. (2011)
  • Author(s): Nakamura, K., H.Tamaki, M.S.Kang, H.Mochimaru, S-T Lee, K.Nakamura, Y.Kamagata
  • Journal Title: Microbes and Environments published on line
  • Peer Reviewed
• [Journal Article] Evidence for syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis in the high-temperature petroleum reservoir of Yabase oil field (Japan). (2011)
  • Author(s): Mayumi, D., H.Mochimaru, S.Sakata, H.Maeda, Y.Miyagawa, M.Ikarashi, M.Takeuchi, Y.Kamagata
  • Journal Title: Environmental Microbiology published on line
  • Peer Reviewed
• [Journal Article] Research for microbial conversion of residual oil into methane in depleted oil fields to develop new EOR process. (2010)
  • Author(s): Maeda H., Y.Miyagawa, M.Ikarashi, D.Mayumi, H.Mochimaru, H.Yoshioka, S.Sakata H.Kobayashi, K.Sato
  • Journal Title: Proceedings of Abudabi Oil and Gas Conference and Exhibition 2010, Abudabi. OnePetro, No.138135-MS
• [Journal Article] Cultivation of methanogens under low hydrogen conditions by using the coculture method. (2009)
  • Author(s): Sakai, S., H.Imachi, Y.Sekiguchi, I-Cheng Tseng, A.Ohashi, H.Harada, Y.Kamagata
  • Journal Title: Applied and Environmental Microbiology 75 Pages: 4892-4896
  • Peer Reviewed
• [Journal Article] Methanolobus profundi sp. nov., a new methylotrophic methanogen isolated from deep subsurface sediments in a natural gas field. (2009)
  • Author(s): Mochimaru, H, H.Tamaki, S.Hanada, H.Imachi, K.Nakamura, S.Sakata, Y.Kamagata
  • Journal Title: International Journal of Systematic and Evolutionary Microbiolology 58 Pages: 714-718
  • Peer Reviewed
• [Presentation] Cultivation and large-scale community analysis of methantrophic organisms. (2011)
  • Author(s): 鎌形洋一
  • Organizer: Hong Kong University special lecture.
  • Place of Presentation: Hong Kong University
  • Year and Date: 2011-03-19
• [Presentation] Anaerobic oxidation of methane in the terrestrial subsurface environment. (2010)
  • Author(s): Takeuchi M., H.Yoshioka, Y.Seo, S.Tanabe, H.Tamaki , Y.Kamagata, H.A.Takahashi, S.Igari, D.Mayumi, S.Sakata
  • Organizer: AGU fall meeting,
  • Place of Presentation: San Francisco
  • Year and Date: 2010-12-14
• [Presentation] 安定同位体トレーサー法を用いた油層内共生微生物によるメタン生成経路の解明 (2010)
  • Author(s): 眞弓大介・坂田将・前田治男・宮川喜洋・五十嵐雅之
  • Organizer: 第2回メタンハイドレート総合シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-12-02
• [Presentation] 深部地下油層環境における原油分解とメタン生成 (2010)
  • Author(s): 持丸華子・眞弓大介・吉岡秀佳・坂田将,玉木秀幸・鎌形洋一
  • Organizer: 第26回日本微生物生態学会
  • Place of Presentation: つくば,茨城
  • Year and Date: 2010-11-24
• [Presentation] :安定同位体トレーサー法を用いた油層内共生微生物によるメタン生成経路の解明 (2010)
  • Author(s): 眞弓大介・坂田将・前田治男・宮川喜洋・五十嵐雅之
  • Organizer: 第26回日本微生物生態学会
  • Place of Presentation: つくば,茨城
  • Year and Date: 2010-11-24
• [Presentation] Raman-FISHを用いた微生物共生系における炭素・窒素の移動経路の解明 (2010)
  • Author(s): 徐維那・竹内美緒・鎌形洋一・坂田将
  • Organizer: 第26回日本微生物生態学会大会
  • Place of Presentation: つくば,茨城
  • Year and Date: 2010-11-24
• [Presentation] Syntrophy based upon interspecies H2 transfer in anaerobic digestion. (2010)
  • Author(s): 鎌形洋一
  • Organizer: International Symposium on Environment and Renewable Energy.
  • Place of Presentation: Pusan National Univ.
  • Year and Date: 2010-10-25
• [Presentation] Anaerobic oxidation of methane in the terrestrial subsurface environment. (2010)
  • Author(s): Takeuchi M., H.Yoshioka, Y.Seo, S.Tanabe, H.Tamaki, Y.Kamagata, H.A.Takahashi, S.Igari, D.Mayumi, S.Sakata
  • Organizer: 13^<th> International Symposium on Microbial Ecology.
  • Place of Presentation: Seatle, USA
  • Year and Date: 2010-08-24
• [Presentation] Methanogenic pathway in a high-temperature petroleum reservoir assessed by stable isotope tracer and molecular biological techniques. (2010)
  • Author(s): 眞弓大介, 坂田将, 坂田将, 宮川喜洋, 五十嵐雅之
  • Organizer: 13^<th> International Symposium on Microbial Ecology.
  • Place of Presentation: Seatle, USA
  • Year and Date: 2010-08-24
• [Presentation] 陸域地下圏における微生物による嫌気的メタン酸化の解明 (2010)
  • Author(s): 竹内美緒・吉岡秀佳・徐維那・田邉晋・玉木秀幸・鎌形洋一・高橋浩・猪狩俊一郎・眞弓大介・坂田将
  • Organizer: 2010年度日本地球惑星科学連合大会
  • Place of Presentation: 幕張,千葉
  • Year and Date: 2010-05-25
• [Book] 難培養微生物研究の最新技術II (2010)
  • Author(s): 鎌形洋一
  • Publisher: シーエムシー出版(分担執筆)