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2011 Fiscal Year Final Research Report

Highly quantitative gene analysis based on affinity capillary electrophoresis using DNA conjugates

Research Project

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Project/Area Number 20245020
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

MAEDA Mizuo  独立行政法人理化学研究所, 前田バイオ工学研究室, 主任研究員 (10165657)

Co-Investigator(Kenkyū-buntansha) TAKARADA Tohru  独立行政法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (30336010)
Project Period (FY) 2008 – 2011
Keywordsバイオ関連機器 / 核酸 / 高分子合成 / アフィニティー分離 / 電気泳動
Research Abstract

To quantitatively detect point gene mutation, we have developed an affinity capillary electrophoresis using a block copolymer probe composed of a synthetic polymer and a single-stranded DNA. During electrophoresis of a mixture of the target and nontarget DNA, the affinity probe binds selectively to the target DNA and thus decreases the electrophoretic mobility by exerting a large amount of hydrodynamic friction. Moreover, this study demonstrated that(1) the peak resolution was improved in the presence of electroosmotic flow and(2) a block copolymer probe containing peptide nucleic acid sequence-selectively bound to secondary-structured target DNA.

  • Research Products

    (9 results)

All 2011 2010 2009 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (5 results) Remarks (1 results)

  • [Journal Article] RAFT-generated polyacrylamide-DNA block copolymers for single-nucleotide polymorphism genotyping by affinity capillary electrophoresis2009

    • Author(s)
      Naoki Kanayama, Hideaki Shibata, Ayumi Kimura, Daisuke Miyamoto, Tohru Takarada, and Mizuo Maeda
    • Journal Title

      Biomacromolecules

      Volume: 10(4) Pages: 805-813

    • Peer Reviewed
  • [Journal Article] Estimation of binding constants of PNA and secondary-structured DNA by affinity capillary electrophoresis

    • Author(s)
      Lal Mohan Kundu, Harumi Tsukada, Yukiharu Matsuoka, Naoki Kanayama, Tohru Takarada, and Mizuo Maeda
    • Journal Title

      Anal. Chem.

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Quantitative SNP genotyping by affinity capillary electrophoresis using PEG-oligodeoxyribonucleotide block copolymers with electroosmotic flow

    • Author(s)
      Harumi Tsukada, Takashi Watanabe, Naoki Kanayama, Tohru Takarada, and Mizuo Maeda
    • Journal Title

      Electrophoresis

      Volume: (in press)

    • Peer Reviewed
  • [Presentation] Affinity capillary electrophoresis for quantitative gene diagnostics2011

    • Author(s)
      宝田徹、前田瑞夫
    • Organizer
      IUPAC International Congress on Analytical Sciences
    • Place of Presentation
      京都府京都市
    • Year and Date
      2011-05-26
  • [Presentation] DNA-conjugated polymers for quantitative SNP analysis2011

    • Author(s)
      宝田徹、前田瑞夫
    • Organizer
      11^<th> International Symposium on Biomimetic Materials Processing
    • Place of Presentation
      愛知県名古屋市
    • Year and Date
      2011-01-27
  • [Presentation] DNA block copolymer probes for affinity electrophoresis in gene diagnosis2010

    • Author(s)
      宝田徹
    • Organizer
      2010 Japan-Taiwan Bilateral Polymer Symposium
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2010-07-01
  • [Presentation] DNA conjugate polymers and nanoparticles for rapid and reliable gene sensing2009

    • Author(s)
      前田瑞夫
    • Organizer
      The 4^<th> Asian and Pacific Rim Symposium on Biophotonics
    • Place of Presentation
      韓国済州島
    • Year and Date
      2009-05-27
  • [Presentation] DNAナノ材料の開発と精密バイオセンシングへの展開2009

    • Author(s)
      前田瑞夫
    • Organizer
      日本化学会第89春季年会
    • Place of Presentation
      千葉県船橋市
    • Year and Date
      2009-03-27
  • [Remarks]

    • URL

      http://www.riken.jp/lab-www/bioengineering/

URL: 

Published: 2013-07-31  

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