2011 Fiscal Year Final Research Report
Highly quantitative gene analysis based on affinity capillary electrophoresis using DNA conjugates
| Project/Area Number |
20245020
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MAEDA Mizuo 独立行政法人理化学研究所, 前田バイオ工学研究室, 主任研究員 (10165657)
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| Co-Investigator(Kenkyū-buntansha) |
TAKARADA Tohru 独立行政法人理化学研究所, 前田バイオ工学研究室, 専任研究員 (30336010)
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| Project Period (FY) |
2008 – 2011
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| Keywords | バイオ関連機器 / 核酸 / 高分子合成 / アフィニティー分離 / 電気泳動 |
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| Research Abstract |
To quantitatively detect point gene mutation, we have developed an affinity capillary electrophoresis using a block copolymer probe composed of a synthetic polymer and a single-stranded DNA. During electrophoresis of a mixture of the target and nontarget DNA, the affinity probe binds selectively to the target DNA and thus decreases the electrophoretic mobility by exerting a large amount of hydrodynamic friction. Moreover, this study demonstrated that(1) the peak resolution was improved in the presence of electroosmotic flow and(2) a block copolymer probe containing peptide nucleic acid sequence-selectively bound to secondary-structured target DNA.
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