2011 Fiscal Year Final Research Report
The construction for recycle of fluorine resource with the complex method
| Project/Area Number |
20245032
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Environmental chemistry
|
|---|
| Research Institution | Tokyo Institute of Technology |
|---|
Principal Investigator |
KITAZUME Tomoya 東京工業大学, 大学院・生命理工学研究科, 教授 (30092547)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
IWAI Noritaka 東京工業大学, 大学院・生命理工学研究科, 助教 (80376938)
|
|---|
| Project Period (FY) |
2008 – 2011
|
| Keywords | 酵素反応 / 5'-FDA / 5-FDR / 連続反応 / 資源循環 / フッ素系物質 / 循環システム |
|---|
| Research Abstract |
We have found the isolation of bacterial strains capable of degrading fluorinated materials, 8 strains of Actinobacteria exhibited degradability of ethyl difluoroacetate(DFAc) was accumulated by bacteria, giving difluoroacetic acid and then fluoride ion. Further, 13 strains of Actinobacteria exhibited degradability of fluorobenzene and/or benzotrifluoride. In batch culture, growth of strains on fluorinated materials led to the release of fluoride ion. We have established the one-pot three-step continuous enzymatic synthesis of 5-fluoro-5-deoxy-D-ribose(5-FDR) from ATP and L-methionine using S-adenosyl-L-methionine synthase(MetK), fluorinase and methylthioadenosinenucleosidase(MtnN) in the presence of fluoride ion. Especially, for the purpose of thesustainable development of fluorine chemistry, we have established the reuse of fluoride ion generated from BF4 ionic liquids and/or the biodegradation of BTF in the one-pot synthetic process to 5-FDR. Further, we have established the retransformation of fluoride ion generated from biodegradation of BTF to calcium fluoride.
|
