2010 Fiscal Year Final Research Report
Evaluation and dynamic measurement of biomembrane transporter using on-chip tethered microtool
| Project/Area Number |
20246044
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Intelligent mechanics/Mechanical systems
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| Research Institution | Tohoku University |
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Principal Investigator |
ARAI Fumihito Tohoku University, 工学研究科, 教授 (90221051)
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| Co-Investigator(Kenkyū-buntansha) |
UOZUMI Nobuyuki 東北大学, 大学院・工学研究科, 教授 (40223515)
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| Co-Investigator(Renkei-kenkyūsha) |
MARUYAMA Hisataka 名古屋大学, 大学院・工学研究科, 助教 (60377843)
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| Project Period (FY) |
2008 – 2010
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| Keywords | マイクロ・ナノデバイス / 光ピンセット / 先端機能デバイス / ナノバイオ / フォトリソグラフィ / イオンチャネル / マイクロ流体デバイス / 遺伝子操作 |
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| Research Abstract |
We promoted the research on basic technologies for on-chip single cell analysis to measure the cell variations induced by control of the environmental conditions and physical stimulus using functional microtool. We fabricated the microfluidic chip having local cell cage by micro-processing based on photofabrication. Functional microtools such as tethered shape tool, flexible tool and sensing tool, were also fabricated. We succeeded in quantitative evaluation of biomembrane transporter by measuring the volume variation of the cell induced by osmotic stimulus on this system.
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[Journal Article] The KtrA and KtrE subunits are required for Na+ dependent K+ uptake by KtrB across the plasma membrane in Synechocystis sp.PCC6803.2010
Author(s)
Zulkifli, L., Akai, M., Yoshikawa, A., Shimojima, M., Ohta, H., Guy HR., Uozumi, N.
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Journal Title
The Journal of Bacteriology Vol.192
Pages: 5063-5070
Peer Reviewed
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[Journal Article] Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SnRK2/OST1/SnRK2.6 protein kinase2009
Author(s)
Sato, A., Sato, Y., Fukao, Y., Fujiwara, M.Umezawa, T., Shinozaki, K., Hibi, T., Taniguchi, M, Miyake, H., Goto, D.B., Uozumi, N.
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Journal Title
Biochem.J. Vol.22
Pages: 439-448
Peer Reviewed
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