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2010 Fiscal Year Final Research Report

Development of functional nano-particles using biomineralization process in magnetic bacteria

Research Project

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Project/Area Number 20246119
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

MATSUNAGA Tadashi  Tokyo University of Agriculture and Technology, 大学院・工学研究院, 教授 (10134834)

Co-Investigator(Kenkyū-buntansha) ARAKAKI Atsushi  東京農工大学, 大学院・工学研究院, 助教 (10367154)
Project Period (FY) 2008 – 2010
Keywordsバイオミネラリゼーション / バイオミメティクス / 結晶核形成 / 微小反応場 / 高保磁力磁性材料 / 機能性ペプチド
Research Abstract

We developed a method for synthesis of functional nano-particles using proteins involved in magnetite biomineralization of magnetotactic bacteria. Shape, size and magnetic property of nano-particles were regulated by using proteins responsible for magnetite crystal formation. Bar domain protein was used to control space for nano-crystal synthesis, and iron transporter protein was used for establishment of sequential crystal formation process in liposomes. The nano-particles developed in this study will be used as nano-materials in the fields of biotechnology and magnetic recordings.

  • Research Products

    (27 results)

All 2011 2010 2009 2008 Other

All Journal Article (22 results) (of which Peer Reviewed: 20 results) Presentation (2 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Mms6 Protein Regulates Crystal Morphology during Nano-sized Magnetite Biomineralization in Vivo.2011

    • Author(s)
      M.Tanaka, E.Mazuyama, A.Arakaki, T.Matsunaga
    • Journal Title

      J.Biol.Chem. 286

      Pages: 6386-6392

    • Peer Reviewed
  • [Journal Article] In Vivo Biotinylation of Bacterial Magnetic Particles by Truncated form of Escherichia coli Biotin Ligase and Biotin Acceptor Peptide.2010

    • Author(s)
      Y.Maeda, T.Yoshino, T.Matsunaga
    • Journal Title

      Appl.Environ.Microbial. 76

      Pages: 5785-5790

    • Peer Reviewed
  • [Journal Article] Surface Modification of Bacterial Magnetic Nanoparticles Using Asparagines-serine Polypeptide Designed to Control Interactions with Cell Surface.2010

    • Author(s)
      M.Takahashi, T.Yoshino, T.Matsunaga
    • Journal Title

      Biomaterials 31

      Pages: 4952-4957

    • Peer Reviewed
  • [Journal Article] Simultaneously Discrete Biomineralization of Magnetite and Tellurium Nanocrystals in Magnetotactic Bacteria.2010

    • Author(s)
      新垣篤史, SS.Stanilan, T.Matsunaga
    • Journal Title

      Appl.Environ. 76

      Pages: 5526-5532

    • Peer Reviewed
  • [Journal Article] Identification and Functional Characterization of Liposome Tubulation Protein from Magnetotactic Bacteria.2010

    • Author(s)
      M.Tanaka, A.Arakaki, T.Matsunaga
    • Journal Title

      Mol.Microbiol. 76

      Pages: 480-488

    • Peer Reviewed
  • [Journal Article] Preparation of Genomic DNA from a Single Species of Uncultured Magnetotactic Bacterium by Multiple Displacement Amplification.2010

    • Author(s)
      A.Arakaki, M.Shibusawa, M.Hosokawa, T.Matsunaga
    • Journal Title

      Appl.Environ.Microbiol. 76

      Pages: 1480-1485

    • Peer Reviewed
  • [Journal Article] Tnducible Expression of Transmembrane Proteins on Bacterial Magnetic Particles in Magnetospirillum magneticum AMB-12010

    • Author(s)
      T.Yoshino, A.Shimojo, Y.Maeda, T.Matsunaga
    • Journal Title

      Appl.Environ.Microbiol. 76

      Pages: 1152-1157

    • Peer Reviewed
  • [Journal Article] Three-dimensional Directed Self-assembly of Peptide Nanowires into Micrometer-sized Crystalline Cubes with Nanoparticle Joints.2010

    • Author(s)
      P.Kaur, Y.Maeda, AC.Mutter, T.Matsunaga, Y.Xu, H.Matsui
    • Journal Title

      Angew.Chem.Int.Ed.Engl. 49

      Pages: 8375-8378

    • Peer Reviewed
  • [Journal Article] Control of the Morphology and Size of Magnetite Particles with Peptides Mimicking the Mms6 Protein from Magnetotactic Bacteria.2010

    • Author(s)
      A.Arakaki, F.Masuda, Y.Amemiya, T.Tanaka, T.Matsunaga
    • Journal Title

      J.Colloid Int.Sci. 343

      Pages: 65-70

    • Peer Reviewed
  • [Journal Article] ナノ磁石を合成する細胞内小器官化学2010

    • Author(s)
      新垣篤史
    • Journal Title

      65

      Pages: 68-69

  • [Journal Article] 磁石を作る細菌のゲノム2010

    • Author(s)
      松永是, 新垣篤史
    • Journal Title

      生物の科学 64

      Pages: 85-91

  • [Journal Article] Whole Genome Sequence of Desulfovibrio magneticus Strain RS-1 Revealed Common Gene Clusters in Magnetotactic Bacteria.2009

    • Author(s)
      H.Nakazawa, A.Arakaki, S.Narita-Yamada, I.Yashiro, K.Jinno, N.Aoki, A.Tsuruyama, Y.Okamura, S.Tanikawa, N.Fujita, H.Takeyama, T.Matsunaga
    • Journal Title

      Genome Res. 19

      Pages: 1801-1808

    • Peer Reviewed
  • [Journal Article] Novel Nanocomposites Consisting of in Vivo-biotinylated Bacterial Magnetic Particles and Quantum Dots for Magnetic Separation and Fluorescent Labeling of Cancer Cells.2009

    • Author(s)
      Y.Maeda, T.Yoshino, T.Matsunaga
    • Journal Title

      J.Mater.Chem. 19

      Pages: 6361-6366

    • Peer Reviewed
  • [Journal Article] Magnetic Separation of Human Podocalyxin-like Protein 1 (hPCLP1)-positive Cells from Peripheral Blood Using Anti-hPCLP1 Monoclonal Antibody and Protein A Expressed on Bacterial Magnetic Particles.2009

    • Author(s)
      M.Kuhara, T.Yoshino, M.Shiokawa, T.Okabe, S.Mizoguchi, A.Yabuhara, H.Takeyama, T.Matsunaga
    • Journal Title

      Cell Struct.Funct. 34

      Pages: 23-30

    • Peer Reviewed
  • [Journal Article] Proteomic Analysis of Irregular Bullet-shaped Magnetosomes in the Sulfate-reducing Bacterium Desulfovibrio magneticus RS-1.2009

    • Author(s)
      T.Matsunaga, M.Nemoto, A.Arakaki, M.Tanaka
    • Journal Title

      Proteomics 9

      Pages: 3341-3352

    • Peer Reviewed
  • [Journal Article] Nano-sized Bacterial Magnetic Particles Displaying Pyruvate Phosphate Dikinase for Pyrosequencing.2009

    • Author(s)
      T.Yoshino, T.Nishimura, T.Mori, S.Suzuki, H.Kambara, H.Takeyama, T.Matsunaga
    • Journal Title

      Biotechnol.Bioeng. 103

      Pages: 130-137

    • Peer Reviewed
  • [Journal Article] Magnetic Separation of Melanoma-specific Cytotoxic T Lymphocytes from a Vaccinated Melanoma Patient's Blood Using MHC/Peptide Complex-conjugated Bacterial Magnetic Particles.2009

    • Author(s)
      M.Takahashi, Y.Akiyama, J.Ikezumi, T.Nagata, T.Yoshino, A.Iizuka, K.Yamaguchi, T.Matsunaga
    • Journal Title

      Bioconjugate Chem. 20

      Pages: 304-309

    • Peer Reviewed
  • [Journal Article] Direct Magnetic Separation of Immune Cells from Whole Blood Using Bacterial Magnetic Particles Displaying ProteinG.2009

    • Author(s)
      M.Takahashi, T.Yoshino, H.Takeyama, T.Matsunaga
    • Journal Title

      Biotechnol.Prog. 25

      Pages: 219-226

    • Peer Reviewed
  • [Journal Article] Noncovalent Immobilization of Streptaviden on In Vitro- and In Vivo-biotinylated Bacterial Magnetic Particles.2008

    • Author(s)
      Y.Maeda, T.Yoshino, M.Takahashi, H.Ginya, J.Asahina, H.Tajima, I.Matsunaga
    • Journal Title

      Appl.Environ.Microbiol. 74

      Pages: 5139-5145

    • Peer Reviewed
  • [Journal Article] Novel Method for Evaluation of Chemicals Based on Ligand-dependent Recruitment of GFP Labeled Coactivator to Estrogen Receptor Displayed on Bacterial Magnetic Particles.2008

    • Author(s)
      T.Yoshino, C.Kajia, M.Nakai, F.Saito, H.Takeyama, T.Matsunaga
    • Journal Title

      Anal.Chim.Acta 626

      Pages: 71-77

    • Peer Reviewed
  • [Journal Article] Magnetic Cell Separation Using Nano-sized Bacterial Magnetic Particles with Reconstructed Magnetosome Membrane2008

    • Author(s)
      T.Yoshino, H.Hirabe, M.Takahashi, M.Kuhara, H.Takeyama, T.Matsunaga
    • Journal Title

      Biotechnol.Bioeng. 101

      Pages: 470-477

    • Peer Reviewed
  • [Journal Article] Novel Method for Selection of Antimicrobial Peptides from a Phage Display Library by Use of Bacterial Magnetic Particles2008

    • Author(s)
      T.Tanaka, Y.Kokuryu, T.Matsunaga
    • Journal Title

      Appl.Environ.Microbiol. 74

      Pages: 7600-7606

    • Peer Reviewed
  • [Presentation] Controlled Mechanism of Iron Oxide Nano-Crystal Formation in Magnetotactic Bacteria2009

    • Author(s)
      T.Matsunaga
    • Organizer
      14th International Conference on Biological Inorganic Chemistry
    • Place of Presentation
      Nagoya Congress Center (Nagoya)
    • Year and Date
      2009-07-29
  • [Presentation] Bacterial Magnetite as an Element in Nanotechnology2008

    • Author(s)
      T.Matsunaga
    • Organizer
      Gordon Research Conference
    • Place of Presentation
      New London (USA)
    • Year and Date
      2008-08-13
  • [Book] 磁性細菌のゲノム情報に基づいた機能性ナノ磁性材料の開発(マリンメタゲノムの有効利用)2009

    • Author(s)
      新垣篤史
    • Total Pages
      13
    • Publisher
      シーエムシー出版
  • [Book] 表面技術2008

    • Author(s)
      松永是
    • Total Pages
      5
    • Publisher
      バイオナノ磁気ビーズの医療応用
  • [Remarks] ホームページ等

    • URL

      http://www.tuat.ac.jp/~matunaga/

URL: 

Published: 2012-01-26   Modified: 2016-04-21  

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