Molecular mechanisms of ovarian differentiation induced by sex hormones

2011 Fiscal Year Final Research Report

• Project/Area Number: 20247004
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Ehime University (2011); National Institute for Basic Biology (2008-2010)
• Principal Investigator: NAGAHAMA Yoshitaka 愛媛大学, 南予水産研究センター, 教授 (50113428)
• Project Period (FY): 2008 – 2011
• Keywords: 卵巣分化 / エストロゲン/エストロゲン受容体 / R-spondin1 / 減数分裂開始 / 魚類 / 遺伝子ノックダウン(RNAi) / C21 steroid / 17α,20β-ジヒドロキシ-4-プレグネン-3-オン(DHP)

Research Abstract

Molecular mechanisms of sex hormone-induced ovarian differentiation were investigated in two fishes (medaka and tilapia). Our results show that estrogen (E2) induces ovarian differentiation by acting through estrogen receptors (β2 and β1) and R-spondin1. We also suggest that P450c17-II (a steroidogenic enzyme necessary for the production of DHP, maturation-inducing hormone of fish) is involved in the initiation of meiosis in both XX and XY fish.

Research Products

• [Journal Article] R-spondins are involved in the ovarian differentiation in a teleost, medaka (Oryzias latipes) (2012)
  • Author(s): Zhu, L.Y., Wang, D.S., Charkraborty, T., Mohapatra, S. and Nagahama, Y
  • Journal Title: BMC Dev. Biol. Volume: 12 Pages: 36
  • DOI: doi: 10.1186/1471-213X-12-36.
  • Peer Reviewed
• [Journal Article] The forkhead gene family in medaka : expression patterns and gene evolution. Cytogenet (2012)
  • Author(s): Shen, X., Cui, J. and Nagahama Y
  • Journal Title: Genome Res. Volume: 136 Pages: 123-130
  • DOI: doi: 10.1159/000335898.
  • Peer Reviewed
• [Journal Article] Expression patterns of gonadotropin hormones and their receptors during early sexual differentiation in Nile tilapia Oreochromis niloticus (2012)
  • Author(s): Yan, H., Ijiri, S., Wu, Q., Kobayashi, T., Li, S., Nakaseko, T., Adachi, S. and Nagahama, Y
  • Journal Title: Biol. Reprod Volume: 87 Pages: 116-126
  • DOI: doi: 10.1095/biolreprod.112.101220.
  • Peer Reviewed
• [Journal Article] Expression of 3β-hydroxysteroid dehydrogenase (hsd3β), star and ad4bp/sf-1 during gonadal development in medaka (Oryzias latipes (2012)
  • Author(s): Nakamoto, M., Fukasawa, M., Tanaka, S., Shimamori, K., Suzuki, A., Matsuda, M., Kobayashi, T., Nagahama, Y. and Shibata N
  • Journal Title: Gen. Comp. Endocrinol Volume: 176 Pages: 222-230
  • DOI: doi: 10.1016/j.ygcen.2012.01.019.
  • Peer Reviewed
• [Journal Article] Dmrt1 mutation causes a male-to-female sex reversal after the sex determination by Dmy in the medaka (2012)
  • Author(s): Masuyama, H., Yamada, M., Kamei, Y., Ishikawa, T., Todo, T., Nagahama, Y. and Matsuda, M
  • Journal Title: Chromosome Res. Volume: 20 Pages: 163-176
  • DOI: doi:10.1007/s10577-011-9264-x.
  • Peer Reviewed
• [Journal Article] Exposure to diethylstilbesterol during embryonic and larval stages of medaka fish (Oryzias latipes) leads to sex reversal in genetic males and reduced gonad weight in genetic females (2011)
  • Author(s): Paul-Prasanth, B., Shibata, Y., Horiguchi, R. and Nagahama, Y
  • Journal Title: Endocrinology Volume: 115 Pages: 707-717
  • DOI: doi:10.1210/en.2010-0812.
  • Peer Reviewed
• [Journal Article] Differential expression of three estrogen receptor subtype mRNAs in gonads and liver from embryos to adults of the medaka, Oryzias latipes (2011)
  • Author(s): Charkraborty, T., Shibata, Y., Zhou, L.Y., Katsu, Y., Iguchi, T. and Nagahama, Y
  • Journal Title: Mol. Cell. Endocrinol Volume: 333 Pages: 47-54
  • DOI: doi:10.1016/j.mce.2010.12.002.
  • Peer Reviewed
• [Journal Article] Estrogen receptors in medaka (Oryzias latipes) and estrogenic environmental contaminants : An in vitro-in vivo correlation (2011)
  • Author(s): Charkraborty, T., Katsu, Y., Zhou, L.Y., Miyagawa, S., Nagahama, Y. and Iguchi, T
  • Journal Title: J. Steroid Biochem. Mol. Biol. Volume: 123 Pages: 115-121
  • DOI: doi:10.1016/j.jsbmb. 2010.11. 015.
  • Peer Reviewed
• [Journal Article] Cloning and expression of medaka cholesterol side chain cleavage cytochrome P450 during gonadal development. Develop (2010)
  • Author(s): Nakamoto, M., Fukasawa, M., Orii, S., Shimamori, K., Maeda, T., Suzuki, A., Matsuda, M., Kobayashi, T., Nagahama, Y. and Shibata, N
  • Journal Title: Growth Differ Volume: 52 Pages: 385-395
  • DOI: doi: 10.1111/j.1440-169X.2010.01178.x.
  • Peer Reviewed
• [Journal Article] Doublesex- and mab-3-Related transcription factor-1 repression of aromatase transcription, a possible mechanism favoring the male pathway in tilapia (2010)
  • Author(s): Wang, D.S., Zhou, L.Y., Kobayashi, T., Matsuda, M., Shibata, Y., Sakai, F. and Nagahama, Y
  • Journal Title: Endocrinology Volume: 151 Pages: 1331-1340
  • DOI: doi: 10.1210/en.2009-0999.
  • Peer Reviewed
• [Journal Article] Homologymodeled ligand-binding domains of medaka estrogen receptors and androgen receptors : a model system for the study of reproduction. Biochem (2009)
  • Author(s): Cui, J.Z., Shen, X.Y., Yan, Z.W., Zhao, H.B. and Nagahama, Y
  • Journal Title: Biophys. Res. Comm. Volume: 380 Pages: 115-121
  • DOI: doi: 10.1016/j.bbrc.2009.01.047.
  • Peer Reviewed
• [Journal Article] Gonadal sex differentiation and expression of Sox9a2, Dmrt1, and Foxl2 in Oryzias luzonensis (2009)
  • Author(s): Nakamoto, M., Muramatsu, S., Yoshida, S., Matsuda, M., Nagahama, Y. and Shibata, N
  • Journal Title: Genesis Volume: 47 Pages: 289-299
  • DOI: doi: 10.1002/dvg.20498.
  • Peer Reviewed
• [Journal Article] Molecular aspects of gonadal differentiation in a teleost fish, the Nile tilapia (2009)
  • Author(s): Kobayashi, T. and Nagahama, Y
  • Journal Title: Sex. Dev. Volume: 3 Pages: 108-117
  • DOI: doi: 10.1159/000223076.
  • Peer Reviewed
• [Presentation] Genetic and hormonal regulation of gonadal development and sexual plasticity in fish (2013)
  • Author(s): Nagahama, Y
  • Organizer: 2013 Society for Integrative and Comparative Biology (SICB) Annual Meeting
  • Place of Presentation: San Francisco CA, USA (Invited lecture)
  • Year and Date: 20130103-07
• [Presentation] 魚類の卵成熟誘起機構 (2012)
  • Author(s): 柴田安司, 成瀬清, 長濱嘉孝
  • Organizer: 日本動物学会中部支部大会
  • Place of Presentation: 松本
  • Year and Date: 20121100
• [Presentation] 魚類の性決定・分化と性的可塑性の分子メカニズム (2012)
  • Author(s): 長濱嘉孝
  • Organizer: 日本動物学会第83回大会シンポジウム
  • Place of Presentation: 大阪大学, 大阪
  • Year and Date: 20120900
• [Presentation] Sex determination/ differentiation and sexual plasticity in fish (2011)
  • Author(s): Nagahama, Y
  • Organizer: Cold Spring Harbor Asia Conference on Developmental Control of Sex, Growth and Cellular Fate
  • Place of Presentation: Suzhou, China (Invited lecture)
  • Year and Date: 20111011-15
• [Presentation] Sex determination/ differentiation and sexual plasticity in fish (2011)
  • Author(s): Nagahama, Y
  • Organizer: The Inaugural Meeting of the North American Society for Comparative Endocrinology
  • Place of Presentation: University of Michigan, Ann Arbor, MI, USA (Invited lecture)
  • Year and Date: 20110613-16
• [Presentation] Gene expression profiling of ovarian follicles in medaka, Oryzias latipes (2011)
  • Author(s): Shibata, Y., Nagahama, Y., Young, G. and Naruse, K
  • Organizer: Center for Reproductive Biology, 15th Annual Retreat
  • Place of Presentation: Washington State University, Pullman, WA, USA
  • Year and Date: 2011-05-19
• [Presentation] Sex determination in fish (2010)
  • Author(s): Nagahama, Y
  • Organizer: Howard Bern Symposium: Recent Advances in Comparative Endocrinology
  • Place of Presentation: Berkeley, CA, USA (Invited lecture)
  • Year and Date: 20100302-03
• [Presentation] Sex determination and sexual plasticity in fish (2010)
  • Author(s): Nagahama, Y
  • Organizer: Satellite International Symposium on Reproductive Biology of Aquatic Organisms
  • Place of Presentation: Motobu-cho, Okinawa, Japan (Invited lecture)
  • Year and Date: 2010-06-30
• [Presentation] Sexual plasticity in fish (2009)
  • Author(s): Nagahama, Y
  • Organizer: Fifth International Symposium on the Biology of Vertebrate Sex Determination
  • Place of Presentation: Kona, Hawaii, USA (Opening lecture)
  • Year and Date: 20090420-24
• [Presentation] R-spondin1 is essential for ovarian differentiation in fish (2009)
  • Author(s): Zhou, L.Y., T. Chakraborty, Wang, D.S. and Nagahama, Y
  • Organizer: 16th International Congress of Comparative Endocrinology
  • Place of Presentation: University of Hong Kong, Hong Kong
  • Year and Date: 2009-06-23
• [Presentation] Mechanisms of sex determination/differentiation and sexual plasticity in fish (2008)
  • Author(s): Nagahama, Y
  • Organizer: 2nd International SOX meeting
  • Place of Presentation: Awaji, Japan (Special lecture)
  • Year and Date: 20080916-19
• [Presentation] Sex determination/ differentiation and sexual plasticity in fish (2008)
  • Author(s): Nagahama, Y
  • Organizer: International Symposium for Gonad and Brain Sex Differentiation
  • Place of Presentation: Fukuoka, Japan (Invited lecture)
  • Year and Date: 20080914-16
• [Presentation] 魚類の性決定・性分化とステロイドホルモン (2008)
  • Author(s): 長濱嘉孝
  • Organizer: 日本動物学会第79回大会シンポジウム
  • Place of Presentation: 福岡大学, 福岡
  • Year and Date: 20080900
• [Presentation] Sex determination/ differentiation and sexual plasticity in fish (2008)
  • Author(s): Nagahama, Y
  • Organizer: XXth International Congress of Zoology
  • Place of Presentation: Paris, France (Invited lecture)
  • Year and Date: 20080826-29
• [Presentation] Expression of a lyasedeficient Cyp17 in the fish head kidney (2008)
  • Author(s): Nagahama, Y.
  • Organizer: XIIIth International Conference on the Adrenal Cortex
  • Place of Presentation: San Francisco, CA, USA (Invited lecture)
  • Year and Date: 20080611-14
• [Presentation] Sex determination, differentiation and sexual plasticity in fish: Current status and future directions (2008)
  • Author(s): Nagahama, Y
  • Organizer: International Symposium on Sex Determination and Gametogenesis in Fish: Current Status and Future Directions
  • Place of Presentation: University of Hawaii, Honolulu, Hawaii, USA (Special lecture)
  • Year and Date: 20080530-0601
• [Presentation] Sexual plasticity in fish (2008)
  • Author(s): Nagahama, Y
  • Organizer: The 54th NIBB Conference on New Frontiers for the Medaka Model Genome
  • Place of Presentation: Okazaki, Japan (Invited lecture)
  • Year and Date: 20080228-29
• [Book] Diversity and plasticity of sex determination and differentiation in fishes. In "Control of Gonadal Development" (Jimenez, R. Ed.) (2012)
  • Author(s): Kobayashi, Y., Nagahama, Y. and Nakamura, M
  • Publisher: Karger(In press)
• [Book] Endocrine regulation of oogenesis in the medaka, Oryzias latipes. In "Medaka : A Model for Organogenesis, Human Disease and Evolution" (H. Takeda et al. eds. (2011)
  • Author(s): Shibata, N., Nakamoto, M., Shibata, Y. and Nagahama, Y.
  • Total Pages: 267-283
  • Publisher: Springer
• [Book] Sex determination in fishes. In "Hormones and Reproduction of Vertebrates" Vol. 1 (D.O. Norris, and K.H. Lopez eds.) (2010)
  • Author(s): Paul-Prasanth, B., Nakamura, M. and Nagahama, Y.
  • Total Pages: 1-14
  • Publisher: Academic Press