2011 Fiscal Year Final Research Report
Crystallographic studies of macromolecular complexes in transcription and translation : RNA polymerases and the ribosome
| Project/Area Number |
20247008
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | The Institute of Physical and Chemical Research (2010-2011)
The University of Tokyo (2009)
Tokyo Gakugei University (2008) |
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Principal Investigator |
YOKOYAMA Shigeyuki 独立行政法人理化学研究所, 生命分子システム基盤研究領域, 領域長 (00159229)
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| Co-Investigator(Kenkyū-buntansha) |
SEKINE Shunichi 東京大学, 大学院・理学系研究科, 講師 (50321774)
ITO Takuhiro 東京大学, 大学院・理学系研究科, 助教 (70401164)
FUJII Yoshifumi 東京大学, 大学院・理学系研究科, 特任助教 (30392096)
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| Co-Investigator(Renkei-kenkyūsha) |
SEKINE Shunichi 東京大学, 大学院・理学系研究科, 講師 (50321774)
ITO Takuhiro 東京大学, 大学院・理学系研究科, 助教 (70401164)
FUJII Yoshifumi 東京大学, 大学院・理学系研究科, 特任助教 (30392096)
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| Project Period (FY) |
2008 – 2011
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| Keywords | 転写 / RNAポリメラーゼ / 転写因子 / 翻訳 / リボソーム / 翻訳開始因子 / X線結晶構造解析 |
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| Research Abstract |
To approach the structural basis of transcription and translation, we carried out structural studies on RNA polymerase and ribosome, which play a central role in transcription and translation, respectively. These include RNA polymerase bound with regulatory proteins and nucleic acids and small ribosomal subunit bound with regulatory proteins and RNA. We also established preparation method of eukaryotic proteins responsible for transcription or translation, and performed crystallography.
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Research Products
(34 results)
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[Journal Article] Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein2010
Author(s)
Tagami, S., Sekine, S., Kumarevel, T., Hino, N., Murayama, Y., Kamegamori, S., Yamamoto, M., Sakamoto, K. and Yokoyama, S.
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Journal Title
Nature
Volume: 468
Pages: 978-982
Peer Reviewed
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[Journal Article] Codon Reassignment in the Escherichia coli Genetic Code2010
Author(s)
Mukai, T., Hayashi, A., Iraha, F., Sato, A., Ohtake, K., Yokoyama, S. and Sakamoto, K.
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Journal Title
Nucleic Acids Res
Volume: 38
Pages: 8188-8195
Peer Reviewed
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[Journal Article] Crystal structure of Methanocaldococcus jannaschii Trm4 complexed with sinefungin2010
Author(s)
Kuratani, M., Hirano, M., Goto-Ito, S., Itoh, Y., Hikida, Y., Nishimoto, M., Sekine, SI., Bessho, Y., Ito, T., Grosjean, H. and Yokoyama, S.
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Journal Title
J. Mol. Biol
Volume: 401(3)
Pages: 323-333
Peer Reviewed
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[Journal Article] Structurally Designed Trans-2-phenylcyclopropylamine Derivatives Potently Inhibit Histone Demethylase LSD1/KDM12010
Author(s)
Mimasu, S., Sato, S., Umezawa, N., Higuchi, T., Umehara, T. and Yokoyama, S.
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Journal Title
Biochemistry
Volume: 49
Pages: 6494-6503
Peer Reviewed
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[Journal Article] The C-terminal BAG Domain of BAG5 Induces Conformational Changes of the Hsp70 Nucleotide-binding Domain for ADP-ATP Exchange2010
Author(s)
Arakawa, A., Handa, N., Ohsawa, N., Shida, M., Kigawa, T., Hayashi, F., Shirouzu, M. and Yokoyama, S.
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Journal Title
Structure
Volume: 18
Pages: 309-319
Peer Reviewed
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[Journal Article] Structure of selenophosphate synthetase essential for selenium incorporation into proteins and RNAs2009
Author(s)
Itoh, Y., Sekine, SI., Matsumoto, E., Akasaka, R., Takemoto, C., Shirouzu, M. and Yokoyama, S.
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Journal Title
J. Mol. Biol
Volume: 385
Pages: 1456-1469
Peer Reviewed
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