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2010 Fiscal Year Final Research Report

The analysis of basic chromosomal functions using human artificial chromosomes (HACs) and the development of novel HACs.

Research Project

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Project/Area Number 20247019
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionKazusa DNA Research Institute

Principal Investigator

MASUMOTO Hiroshi  Kazusa DNA Research Institute, ヒトゲノム研究部, 室長 (70229384)

Co-Investigator(Renkei-kenkyūsha) NAKANO Megumi  (財団法人)かずさDNA研究所, 研究員 (50542825)
OHZEKI Jun-Ichirou  (財団法人)かずさDNA研究所, 研究員 (30514088)
Project Period (FY) 2008 – 2010
Keywords遺伝学 / 染色体 / 分配 / セントロメア / 人工染色体 / クロマチン / エピジェネティックス / ヘテロクロマチン
Research Abstract

We have developed a novel human artificial chromosome (HAC) system to investigate the epigenetic state of chromatin by applying a fusion-protein tethering system. We found that altering the HAC centromere chromatin to a more heterochromatic with the tTS transcription silencer or histone methyl transferase (Suv39h) caused mis-segregation of the HAC by losing kinetochore structure. Otherwise, histone acetyl transferase (HAT) fusions enhanced hyper-assembly of kinetocore components, indicating that a dynamic counterbalance is essential for mammalian kinetochore assembly.

  • Research Products

    (16 results)

All 2011 2010 2009 2008 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (7 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Epigenetic engineering sho ws H3K4 me2 is required for HJURP targeting and CENP-A assembly on a synthetic human kinetochore.2011

    • Author(s)
      Bergmann J.H., Gomez M., Martins N.M.C., Kimura H., Kelly D.A., Masumo to H., Larionov V., Jansen L.E.T., Earnshaw W.C.
    • Journal Title

      EMBO J. 30

      Pages: 328-340

    • Peer Reviewed
  • [Journal Article] Human Artificial Chromosome with a Conditional Centromere for Gene Delivery and Gene Expression2010

    • Author(s)
      Iida Y., Kim JH., Kazuki Y., Hoshiya H., Takiguchi M., Hayashi M., Erliandri I., Lee HS., Samoshkin A., Masumoto H., Earnshaw WC., Kouprina N., Larionov V., Oshimura M.
    • Journal Title

      DNA Res. 17

      Pages: 293-301

    • Peer Reviewed
  • [Journal Article] Hierarchical inactivation of a synthetic human kinetochore by a chromatin modifier.2009

    • Author(s)
      Cardinale S., Bergmann J.H., Kelly D., Nakano M., Valdivia M.M., Kimura H., Masumoto H., Larionov V., Earnshaw W.C.
    • Journal Title

      Mol.Biol.Cell 20

      Pages: 4194-4204

    • Peer Reviewed
  • [Journal Article] Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing.2009

    • Author(s)
      Kim JH., Ebersole T., Kouprina N., Noskov V.N., Ohzeki J., Masumoto H., Mravinac B., Sullivan B.A., Pavlicek A., Dovat S., Pack S.D., Kwon YW., Flanagan P.T., Loukinov D., Lobanenkov V., Larionov V.
    • Journal Title

      Genome Res. 19

      Pages: 533-544

    • Peer Reviewed
  • [Journal Article] Inactivation of a human kinetochore by specific targeting of chromatin modifiers.2008

    • Author(s)
      Nakano M., Cardinale S., Noskov V.N., Gassmann R., Vagnarelli P., Kandels-Lewis S., Larionov V., Earnshaw W.C., Masumoto H.
    • Journal Title

      Dev.Cell 14

      Pages: 507-522

    • Peer Reviewed
  • [Presentation] A chromatin assembly balance determines the fate of de novo kinetochre or heterochromatin formation on satellite DNA.2010

    • Author(s)
      Masumoto H., Ohzeki J., Nakano M., et al. V.
    • Organizer
      EMBO Workshop on Chromosome Segregation and Aneuploidy
    • Place of Presentation
      Edinburgh
    • Year and Date
      20100619-20100623
  • [Presentation] コンディショナルセントロメアを持つ人工染色体2010

    • Author(s)
      舛本寛
    • Organizer
      第82回日本遺伝学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2010-09-22
  • [Presentation] A Dynamic Assembly Balance of Centromere Chromatin and Heterochromatin on Alpha-satellite DNA.2009

    • Author(s)
      舛本寛
    • Organizer
      The American Society of Human Genetics The 59th Annual Meeting, in The Invited Session "Epigenetics of human centromere formation"
    • Place of Presentation
      Honolulu, Hawaii
    • Year and Date
      2009-10-20
  • [Presentation] Dynamic Assembly mechanism of Centromere Chromatin and Heterochromatin on Human Satellite DNA.2009

    • Author(s)
      Masumoto H., Ohzeki J., Nakano M., Okada T., Larionov V., Earnshaw W.C.
    • Organizer
      The 24^<th> Naito Conference on "Nuclear Dynamics and RNA [II]"
    • Place of Presentation
      Sapporo
    • Year and Date
      2009-06-25
  • [Presentation] セントロメア機能形成に関わるダイナミックなクロマチン集合バランス2008

    • Author(s)
      舛本寛、中野めぐみ、大関淳一郎、他
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      20081208-20081212
  • [Presentation] A Dynamic Assembly Balance of CENP-A Chromatin or Heterochromatin for Centromere Activity on Alpha-satellite Repeats.2008

    • Author(s)
      舛本寛, et al.
    • Organizer
      EMBO Workshop Chromosome Segregation : Centromeres and Kinetochores
    • Place of Presentation
      Arcachon, Bordeaux, France
    • Year and Date
      2008-09-28
  • [Presentation] Dynamic Assembly and Inactivation of Human Centromere on Satellite DNA2008

    • Author(s)
      舛本寛, et al.
    • Organizer
      International Symposium on Chromosome Dynamics in Ise
    • Year and Date
      2008-05-28
  • [Book] 染色体分配装置セントロメアの形成メカニズム、蛋白質核酸酵素(54 査読無し)2009

    • Author(s)
      中野めぐみ、舛本寛
    • Total Pages
      427-435
  • [Book] The Kinetochore : from Molecular Discoveries to Cancer Therapy(Eds. De Wulf P., and Earnshaw W.C.)(Human Artificial Centromeres : De novo assembly of functional centromeres on human artificial chromosomes. 査読あり)2008

    • Author(s)
      Masumoto H., Okada T., Okamoto Y.
    • Total Pages
      107-132
    • Publisher
      Springer Publ.New York
  • [Book] ヒト人工染色体から見た染色体制御機構(80 査読無し)2008

    • Author(s)
      舛本寛、岡田晃明
    • Total Pages
      200-209
    • Publisher
      生化学
  • [Remarks] ホームページ等

    • URL

      http://www.kazusa.or.jp/j/laboratories/labo_cellengineering.html

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Published: 2012-01-26   Modified: 2016-04-21  

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