Invasiveness acquired during EMT

2010 Fiscal Year Self-evaluation Report

• Project/Area Number: 20247027
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Hokkaido University (2009-2011); Osaka Bioscience Institute (2008)
• Principal Investigator: SABE Hisataka Osaka Bioscience Institute, 大学院・医学研究科, 教授 (40187282)
• Project Period (FY): 2008 – 2011
• Keywords: EMT / 浸潤 / Arf GTPases / E-cadherin / endocytosis / AMAP1

Research Abstract

上皮間充織形質転換(EMT)は外胚葉由来細胞が中胚葉(間充織)細胞様形質を獲得し、運動性や浸潤性を獲得する過程である。EMT様変化は多くの癌細胞の浸潤転移にも関与し、その分子機構を明らかにする事は生物学的のみならず医学的にも注目されている。EMTの実態をなす根幹の一側面はE-cadherinの不活性化とある種のintegrinの活性化である。本研究ではEMTにおける浸潤性獲得機序の解析を主な目的とする。その為、まずはTGFβ1によるE-cadherinの不活性化の分子機構を明らかにする。

Research Products

• [Journal Article] EphA2 engages Git1 to suppress Arf6 activity modulating epithelial cell-cell contacts (2009)
  • Author(s): Miura K., Nam J.M., Kojima C., Mochizuki N., Sabe H.
  • Journal Title: Mol Biol Cell 20 Pages: 1949-1959
  • Peer Reviewed
• [Journal Article] The EGFR-GEP100-Arf6-AMAP1 signaling pathway specific to breast cancer invasion and metastasis (2009)
  • Author(s): Sabe H., Hashimoto S., Morishige M., Ogawa E., Hashimoto A., Nam J.M., Miura K., Yano H., Onodera Y.
  • Journal Title: Traffic 10 Pages: 982-993
  • Peer Reviewed
• [Journal Article] EPB41L5 functions to post-transcriptionally regulate cadherin and integrin during pithelial-mesenchymal transition (2008)
  • Author(s): Hirano M., Hashimoto S., Yonemura S., Sabe H., Aizawa S.
  • Journal Title: J Cell Biol. 182 Pages: 1217-1230
  • Peer Reviewed
• [Journal Article] Fbx8 makes Arf6 refractory to function via ubiquitination (2008)
  • Author(s): Yano H., obayashi I., Onodera Y., Luton F., Franco M., Mazaki Y., Hashimoto S., Iwai K., Ronai Z., Sabe H.
  • Journal Title: Mol Biol Cell. 19 Pages: 822-832
  • Peer Reviewed
• [Journal Article] GEP100 links epidermal growth factor receptor signalling to Arf6 activation to induce breast cancer invasion (2008)
  • Author(s): Morishige M., Hashimoto S., Ogawa E., Toda Y., Kotani H., Hirose M., Wei S., Hashimoto A., Yamada A., Yano H., Mazaki Y., Kodama H., Nio Y., Manabe T., Wada H., Kobayashi H., Sabe H.
  • Journal Title: Nat Cell Biol. 10 Pages: 85-92
  • Peer Reviewed
• [Presentation] The Arf6-AMAP1 Pathway Contributes to Cancerous EMT (2010)
  • Author(s): Sabe H
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-23
• [Presentation] Integrin activation and E-cadherin inactivation by the Arf6 pathway specific to breast cancer invasion and metastasis (2010)
  • Author(s): Sabe H
  • Organizer: 第6回OOTR年次学会
  • Place of Presentation: 京都
  • Year and Date: 2010-02-25
• [Presentation] メンブレントラフィックと癌浸潤形質獲得機序 (2009)
  • Author(s): Sabe H
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-21
• [Presentation] Integrin activation and E-cadherin inactivation by the Arf6 pathway specific to breast cancer invasion and metastasis. (2009)
  • Author(s): Sabe H
  • Organizer: 2009 FEBS workshop
  • Place of Presentation: Seefeld
  • Year and Date: 2009-09-06