GENETIC ANALYSIS OF AXIS FORMATION IN VERTEBRATES USING MEDAKA

2011 Fiscal Year Final Research Report

• Project/Area Number: 20247030
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: TAKEDA Hiroyuki 東京大学, 大学院・理学系研究科, 教授 (80179647)
• Project Period (FY): 2008 – 2011
• Keywords: 左右軸 / 背腹軸 / メダカ / 突然変異体

Research Abstract

The study has demonstrated the novel mechanisms of left-right axis formation and late dorsoventral patterning using medaka developmental mutants. First, medaka left-right mutants have identified the novel cytoplasmic protein, Ktu required for axonemal dynein assembly together with molecular chaperons. Furthermore, the un-characterized membrane protein Pkd1l1 was found to serve as a flow sensor on motile cilia in the node region at an initial step of left-right determination. Finally, zic1/zic4 have crucial roles in dorsoventral patterning of the trunk region and in tail morphogenesis.

Research Products

• [Journal Article] The Medakazic1/zic4 Mutant Provides MolecularInsights into Teleost Caudal FinEvolution (2012)
  • Author(s): Moriyama, Y., Kawanishi, T., Nakamura, R., Tsukahara, T., et al
  • Journal Title: Curr Biol Volume: 22 Pages: 601-7
  • DOI: DOI:10.1016/j.cub.2012.01.063
  • Peer Reviewed
• [Journal Article] Pkd1l1 complexes withPkd2 on motile cilia and functions toestablish the left-right axis (2011)
  • Author(s): Kamura, K., Kobayashi, D., Uehara, Y., Koshida, S., et al
  • Journal Title: Development Volume: 138 Pages: 1121-9
  • DOI: DOI:10.1242/dev.058271
  • Peer Reviewed
• [Journal Article] The art of medaka genetics and genomics : what makesthem so unique (2010)
  • Author(s): Takeda, H., Shimada, A.
  • Journal Title: Annu Rev Genet Volume: 44 Pages: 217-41
  • DOI: DOI:10.1146/annurevgenet-051710-151001
  • Peer Reviewed
• [Journal Article] Single-cell analysis of somatotopic mapformation in the zebrafish lateral linesystem (2010)
  • Author(s): Sato, A., Koshida, S. & Takeda, H.
  • Journal Title: Dev Dyn Volume: 239 Pages: 2058-65
  • DOI: DOI:10.1002/dvdy.22324
  • Peer Reviewed
• [Journal Article] Emergence of traveling wave in thezebrafish segmentation clock (2010)
  • Author(s): Ishimatsu, K., Takamatsu, A., Takeda, H.
  • Journal Title: Development Volume: 137 Pages: 1595-9
  • DOI: DOI:dev.046888[pii]10.1242/dev.046888
  • Peer Reviewed
• [Journal Article] FAK-mediated extracellularsignals are essential for interkineticnuclear migration and planar divisions inthe neuroepithelium (2010)
  • Author(s): Tsuda, S., Kitagawa, T., Takashima, S., et al
  • Journal Title: J Cell Sci Volume: 123 Pages: 484-96
  • DOI: DOI:jcs.057851[pii]10.1242/Jcs.057851
  • Peer Reviewed
• [Journal Article] Characterization of teleost Mdga1 usinga gene-trap approach in medaka(Oryziaslatipes) (2009)
  • Author(s): Sano, S., Takashima, S., Niwa, H., et al
  • Journal Title: Genesis Volume: 47 Pages: 505-13
  • DOI: DOI:10.1002/dvg.20528
  • Peer Reviewed
• [Journal Article] Ktu/PF13 is required forcytoplasmic pre-assembly of axonemaldyneins (2008)
  • Author(s): Omran, H., Kobayashi, D., Olbrich, H., Tsukahara, T., Loges, N. T., Hagiwara, H., Zhang, Q., Leblond, G., Toole, E. O', Hara, C., Mizuno, H., Kawano, H., Fliegauf, M., Yagi, T., Koshida, S., Miyawaki, A., Zentgraf, H., Seithe, H., Reinhardt, R., Watanabe,
  • Journal Title: Nature Volume: 456 Pages: 611-6
  • DOI: DOI:nature07471[pii]10.1038/nature07471
  • Peer Reviewed
• [Presentation] Epigenetic regulation ofkey developmental genes. genome wideapproaches in medaka fish (2012)
  • Author(s): Takeda, H.
  • Organizer: The firstbilateral India-Japan DevelopmentalBiology Workshop
  • Place of Presentation: バンガロール
  • Year and Date: 2012-01-08
• [Presentation] 発生後期におけるzic1/zic4を介した背腹パターン決定機構 (2011)
  • Author(s): 島田敦子
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] zic1/4は真骨魚類における正型尾形成に必須である (2011)
  • Author(s): 守山裕大
  • Organizer: 日本動物学会第82回旭川大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] Mesodermal origin of scales and fins of the medaka-Insight into origin and evolution of mineralized skeleton in vertebrates (2011)
  • Author(s): 武田洋幸
  • Organizer: JSDB-GFE Joint Meeting ofDevelopmental Bioligy
  • Place of Presentation: ドレスデン
  • Year and Date: 2011-03-24
• [Presentation] Analyses of the cytoplasmicprotein Ktu function in axonemaldyne information during motile ciliogenesis (2010)
  • Author(s): 松尾萌
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Pkd1l1/Pkd2 as nodalflow sensor to regulate left-right asymmetry (2010)
  • Author(s): 加村啓一郎
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Embryonic origin of osteoblasts in scales and fins of medaka fish (2010)
  • Author(s): 島田敦子
  • Organizer: 2010 SDB-JSDB JointMeeting
  • Place of Presentation: アルバカーキ
  • Year and Date: 2010-08-05
• [Presentation] zic1 and zic4 expression in the somite regulates dorsalization of the fish trunk structures (2010)
  • Author(s): 河西通
  • Organizer: 2010 SDB-JSDB Joint Meeting
  • Place of Presentation: アルバカーキ
  • Year and Date: 2010-08-05
• [Presentation] The zic genes as masterregulators in dorsoventral patterning of the fish trunkstructures (2010)
  • Author(s): 守山裕大
  • Organizer: 2^<nd> Joint Meeting of the SFBD and JSDB 2010
  • Place of Presentation: パリ
  • Year and Date: 2010-05-27
• [Presentation] Single-Cell Analysis of Somatotopic Map Formation in the Zebrafish Lateral Line System (2010)
  • Author(s): 佐藤朗
  • Organizer: 2^<nd> Joint Meeting of the SFBD and JSDB 2010
  • Place of Presentation: パリ
  • Year and Date: 2010-05-27
• [Presentation] The zic genes asmaster regulators in dorsoventral patterning of the fish trunkstructures (2010)
  • Author(s): 武田洋幸
  • Organizer: CDBシンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2010-03-24
• [Presentation] Left-right mutantmedaka identifies a candidate sensor of leftward flow (2009)
  • Author(s): 加村啓一郎
  • Organizer: 16^<th> ISDB 2009
  • Place of Presentation: スコットランド
  • Year and Date: 2009-09-06
• [Presentation] 左右軸から繊毛形成まで-メダカ変異体を用いた遺伝学的解析 (2008)
  • Author(s): 武田洋幸
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Analysis of the somite-specific Enhancer of the zic Genes in Medaka : Toward Understanding of the Evolution of the Body Shape in Teleosts (2008)
  • Author(s): 守山裕大
  • Organizer: The 16^<th> CDB Meeting
  • Place of Presentation: 神戸
  • Year and Date: 2008-09-28